Virucidal Efficacy of Physico-chemical Treatments Against Coronaviruses and Parvoviruses of Laboratory Animals

Saknimit, Morakot; Inatsuki, Ikko; Sugiyama, Yuko; Yagami, Ken‐ichi

doi:10.1538/expanim1978.37.3_341

Cited by 117 publications

(137 citation statements)

References 2 publications

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“…2). Stability against ethanol and 2-propanol has also been demonstrated previously in tests with two parvoviruses (CPV and Kilham rat virus), confirming our data [31]. Although in this study, BPV and CPV demonstrated the highest stability, both viruses are difficult to handle in the laboratory.…”

Section: Discussionsupporting

confidence: 90%

Evaluation of a Virucidal Quantitative Carrier Test for Surface Disinfectants

Rabenau

Steinmann

Rapp³

et al. 2014

PLoS ONE

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Surface disinfectants are part of broader preventive strategies preventing the transmission of bacteria, fungi and viruses in medical institutions. To evaluate their virucidal efficacy, these products must be tested with appropriate model viruses with different physico-chemical properties under conditions representing practical application in hospitals.The aim of this study was to evaluate a quantitative carrier assay. Furthermore, different putative model viruses like adenovirus type 5 (AdV-5) and different animal parvoviruses were evaluated with respect to their tenacity and practicability in laboratory handling. To evaluate the robustness of the method, some of the viruses were tested in parallel in different laboratories in a multi-center study. Different biocides, which are common active ingredients of surface disinfectants, were used in the test. After drying on stainless steel discs as the carrier, model viruses were exposed to different concentrations of three alcohols, peracetic acid (PAA) or glutaraldehyde (GDA), with a fixed exposure time of 5 minutes. Residual virus was determined after treatment by endpoint titration.All parvoviruses exhibited a similar stability with respect to GDA, while AdV-5 was more susceptible. For PAA, the porcine parvovirus was more sensitive than the other parvoviruses, and again, AdV-5 presented a higher susceptibility than the parvoviruses. All parvoviruses were resistant to alcohols, while AdV-5 was only stable when treated with 2-propanol. The analysis of the results of the multi-center study showed a high reproducibility of this test system.In conclusion, two viruses with different physico-chemical properties can be recommended as appropriate model viruses for the evaluation of the virucidal efficacy of surface disinfectants: AdV-5, which has a high clinical impact, and murine parvovirus (MVM) with the highest practicability among the parvoviruses tested.

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Section: Discussionsupporting

confidence: 90%

Evaluation of a Virucidal Quantitative Carrier Test for Surface Disinfectants

Rabenau

Steinmann

Rapp³

et al. 2014

PLoS ONE

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“…Virucidal activity of several microbicides was tested against the mouse hepatitis virus (MHV) and the canine coronavirus (CCV), Kilham rat virus and canine parvovirus [32]. Both coronaviruses were readily inactivated by ethanol, isopropanol, benzalkonium chloride, iodophor, sodium hypochlorite, sodium chlorite, cresol soap and formaldehyde whereas the two parvoviruses proved to be considerably more resistant.…”

Section: Activity Of Microbicides Against Coronavirusesmentioning

confidence: 99%

Environmental survival and microbicide inactivation of coronaviruses

Wolff¹,

Sattar²,

Adegbunrin³

et al. 2005

Coronaviruses With Special Emphasis on First Insights Concerning SARS

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“…reported [1,4,[6][7][8][9][10][11][14][15][16]. But only a few studies have been reported on the virucidal efficacy of physicochemical treatments on laboratory animal viruses [12,13,17] .…”

Section: Resultsmentioning

confidence: 99%

“…But little is known about the relative susceptibility of laboratory animal RNA viruses, especially in species of rodents, to physicochemical treatments [6,12,13,15].…”

mentioning

confidence: 99%