Virucidal activity of proanthocyanidin against Mayaro virus

Ferraz, Ariane Coelho; Moraes, Thaís de Fátima Silva; Nizer, Waleska Stephanie da Cruz; Santos, Michelli dos; Tótola, Antônio Helvécio; Ferreira, Jaqueline Maria Siqueira; Filho, Sidney Augusto Vieira; Rodrigues, Vanessa Gregório; Duarte, Lucienir Pains; Magalhães, Cíntia Lopes de Brito; Magalhães, José Carlos de

doi:10.1016/j.antiviral.2019.05.008

Cited by 28 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been previously described those natural compounds with a structural relationship may exhibit differences in their virucidal capacity, as is the case for 4′-O-methylepigallocatechin and proanthocyanidin against the Mayaro virus. The first compound does not present activity, even at the highest concentrations, whereas the second presents potent virucidal activity against this arbovirus [ 46 ]. Likewise, it has been reported that epigallocatechin gallate, which is structurally related to the two previously mentioned compounds, has virucidal activity, acting directly on the ZIKV envelope [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Indole alkaloids inhibit zika and chikungunya virus infection in different cell lines

Monsalve-Escudero

Loaiza-Cano

Pájaro-González

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

Background In recent years, an increase in the occurrence of illnesses caused by two clinically- important arboviruses has been reported: Zika virus (ZIKV) and Chikungunya virus (CHIKV). There is no licensed antiviral treatment for either of the two abovementioned viruses. Bearing in mind that the antiviral effect of indole alkaloids has been reported for other arboviral models, the present study proposed to evaluate the antiviral in vitro and in silico effects of four indole alkaloids on infections by these two viruses in different cell lines. Methods The antiviral effects of voacangine (VOAC), voacangine-7-hydroxyindolenine (VOAC-OH), rupicoline and 3-oxo voacangine (OXO-VOAC) were evaluated in Vero, U937 and A549 cells using different experimental strategies (Pre, Trans, Post and combined treatment). Viral infection was quantified by different methodologies, including infectious viral particles by plating, viral genome by RT-qPCR, and viral protein by cell ELISA. Moreover, molecular docking was used to evaluate the possible interactions between structural and nonstructural viral proteins and the compounds. The results obtained from the antiviral strategies for each experimental condition were compared in all cases with the untreated controls. Statistically significant differences were identified using a parametric Student’s t-test. In all cases, p values below 0.05 (p < 0.05) were considered statistically significant. Results In the pre-treatment strategy in Vero cells, VOAC and VOAC-OH inhibited both viral models and OXO-VOAC inhibited only ZIKV; in U937 cells infected with CHIKV/Col, only VOAC-OH inhibited infection, but none of the compounds had activity in A549 cells; in U937 cells and A549 cells infected with ZIKV/Col, the three compounds that were effective in Vero cells also had antiviral activity. In the trans-treatment strategy, only VOAC-OH was virucidal against ZIKV/Col. In the post-treatment strategy, only rupicoline was effective in the CHIKV/Col model in Vero and A549 cells, whereas VOAC and VOAC-OH inhibited ZIKV infection in all three cell lines. In the combined strategy, VOAC, VOAC-OH and rupicoline inhibited CHIKV/Col and ZIKV/Col, but only rupicoline improved the antiviral effect of ZIKV/Col-infected cultures with respect to the individual strategies. Molecular docking showed that all the compounds had favorable binding energies with the structural proteins E2 and NSP2 (CHIKV) and E and NS5 (ZIKV). Conclusions The present study demonstrates that indole alkaloids are promising antiviral drugs in the process of ZIKV and CHIKV infection; however, the mechanisms of action evaluated in this study would indicate that the effect is different in each viral model and, in turn, dependent on the cell line.

show abstract

Section: Discussionmentioning

confidence: 99%

Indole alkaloids inhibit zika and chikungunya virus infection in different cell lines

Monsalve-Escudero

Loaiza-Cano

Pájaro-González

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…On the other hand, only TDB-2M-ME presented a virucidal effect in the ZIKV model by decreasing the production of infectious viral particles ( Figure 5 ). Virucidal activity has been associated with complex molecules such as proanthocyanidin [ 36 ]; nevertheless, in the Influenza A model, it has been described that small esterified molecules such as oseltamivir and zanamivir [ 37 ] possess virucidal activity through direct binding with the structural protein neuraminidase. Therefore, because TDB-2M-ME is a small, esterified molecule, its virucidal activity could be related to binding to a viral protein involved in viral adhesion with the receptor of the host.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Silico Anti-Arboviral Activities of Dihalogenated Phenolic Derivates of L-Tyrosine

et al. 2021

View full text Add to dashboard Cite

Despite the serious public health problem represented by the diseases caused by dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses, there are still no specific licensed antivirals available for their treatment. Here, we examined the potential anti-arbovirus activity of ten di-halogenated compounds derived from L-tyrosine with modifications in amine and carboxyl groups. The activity of compounds on VERO cell line infection and the possible mechanism of action of the most promising compounds were evaluated. Finally, molecular docking between the compounds and viral and cellular proteins was evaluated in silico with Autodock Vina®, and the molecular dynamic with Gromacs®. Only two compounds (TDC-2M-ME and TDB-2M-ME) inhibited both ZIKV and CHIKV. Within the possible mechanism, in CHIKV, the two compounds decreased the number of genome copies and in the pre-treatment strategy the infectious viral particles. In the ZIKV model, only TDB-2M-ME inhibited the viral protein and demonstrate a virucidal effect. Moreover, in the U937 cell line infected with CHIKV, both compounds inhibited the viral protein and TDB-2M-ME inhibited the viral genome too. Finally, the in silico results showed a favorable binding energy between the compounds and the helicases of both viral models, the NSP3 of CHIKV and cellular proteins DDC and β2 adrenoreceptor.

show abstract

“…In the case of natural compounds, there is an increasing number of potentially useful plants and herbs that need to be exploited and their therapeutic applications are important weapons to be used against most virus families, and even for emerging viruses. The in vitro studies of antiviral effect, therefore, provide the relevant information necessary and the concentrations of the substances to be used for further tests, before it can that go to clinical trials, making it possible to access the risk-bene t issue [3,17,18,26,[30][31][32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Salles¹,

Meneses²,

Caldas³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The arthropod-borne Mayaro virus (MAYV) causes ‘Mayaro fever’, a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. Currently, there are no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil were submitted to a phytochemical investigation for the identification and isolation of antiviral compounds. In the present study we studied the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods: The Ethanol extract and Punicalagin of Pomegranate were extracted solely of the shell and purified by chromatographic fractionation and chemically identified using spectroscopic techniques. Cytotoxicity of purified compounds was measured by the dye-uptake assay while their antiviral activity was evaluated by a virus yield inhibition assay.Results: Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of Pomegranate extract and Transmission Electron Microscopy (TEM) revealed damage in viral particles treated with this extract. Conclusions: The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and are excellent candidates for future studies with other enveloped RNA viruses.

show abstract

Virucidal activity of proanthocyanidin against Mayaro virus

Cited by 28 publications

References 30 publications

Indole alkaloids inhibit zika and chikungunya virus infection in different cell lines

Indole alkaloids inhibit zika and chikungunya virus infection in different cell lines

In Vitro and In Silico Anti-Arboviral Activities of Dihalogenated Phenolic Derivates of L-Tyrosine

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Contact Info

Product

Resources

About