Virucidal activity in aqueous extracts obtained from <i>Cedrela tubiflora</i> leaves

Córdoba, Mónica A.; Coto, Celia E.; Damonte, Elsa B.

doi:10.1002/ptr.2650050604

Cited by 10 publications

(2 citation statements)

References 4 publications

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“…Medicinal plants or extracts of herbs are extensively screened to identify novel molecules able to prevent virus replication (Mantani et al, 1999;Simoes et al, 1999;Yukawa et al, 1996;Hattori et al, 1995). We have found that crude leaf extracts from members of the Meliaceae family display an antiviral activity against various viruses (Có rdoba et al, 1991;Benencia et al, 1997;Andrei et al, 1994). Melia azedarach L. (Meliaceae), also known as the chinaberry tree, is a deciduous tree native to India and has long been recognized for its medicinal and insecticidal properties (Bohnenstengel et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Block of vesicular stomatitis virus endocytic and exocytic pathways by 1-cinnamoyl-3,11-dihydroxymeliacarpin, a tetranortriterpenoid of natural origin

Barquero

Alché

Coto

2004

Journal of General Virology

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Previously, it has been shown that 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), a natural compound isolated from leaf extracts of Melia azedarach L., inhibits the vesicular stomatitis virus (VSV) multiplication cycle when added before or after infection. Here, we have established that the lack of VSV protein synthesis in CDM pre-treated Vero cells is ascribed to the inhibition of an initial step during virus multiplication, although indirect immunofluorescence (IFI) studies confirmed that the binding and uptake of [35 S]methionine-labelled VSV was not affected by CDM pre-treatment. Instead, our findings revealed that this compound impedes the uncoating of VSV nucleocapsids in pre-treated Vero cells, since the antiviral action of CDM was partially reversed by inducing VSV direct fusion at the plasma membrane, and VSV M protein fluorescence was confined to the endosomes, even 2 h post-internalization. Furthermore, CDM induced cytoplasmic alkalinization, as shown by acridine orange staining, consistent with the inhibition of virus uncoating. Although VSV proteins are synthesized when CDM is added after infection, IFI studies revealed that G protein was absent from the surface of infected cells and co-localized with a Golgi marker. Therefore, CDM inhibits the transport of G protein to the plasma membrane. Taken together, these findings indicate that CDM exerts its antiviral action on the endocytic and exocytic pathways of VSV by pre-or post-treatment, respectively.

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Section: Introductionmentioning

confidence: 99%

Block of vesicular stomatitis virus endocytic and exocytic pathways by 1-cinnamoyl-3,11-dihydroxymeliacarpin, a tetranortriterpenoid of natural origin

Barquero

Alché

Coto

2004

Journal of General Virology

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“…In our laboratory, different bioactive compounds with antiviral activity in crude extracts obtained from leaves of members of the Meliaceae family were detected [Wachsman et al, 1988;Córdoba et al, 1991;Benencia et al, 1997]. Meliacine (MA), a peptide of low molecular weight, was isolated from Melia azedarach L. crude leaf extracts [Andrei et al, 1994].…”

Section: Introductionmentioning

confidence: 99%

Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice

et al. 2000

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Herpetic stromal keratitis is caused by ocular infection with herpes simplex virus type 1 (HSV-1) and constitutes a leading cause of human blindness. The effect of meliacine, an antiviral compound isolated from leaves of Melia azedarach L. that inhibits HSV-1 replication in vitro, was examined on experimental corneal HSV-1 inoculation in Balb/c mice. Mice were inoculated with HSV-1 strain KOS at their corneas after abrasion. Meliacine was administered topically 3 times a day for 4 days beginning 1 day before inoculation. Infected animals treated or not with meliacine were observed carefully for the development of stromal keratitis and the clinical scoring was done 14 days post-infection. Histological examination of corneas and viral isolation from eyes from HSV-1 infected mice treated or not with meliacine were also carried out. It was found that the treatment of HSV-1-induced ocular disease in Balb/c mice with meliacine reduced significantly the development of clinical disease, as well as the histological damage in corneas. The viral titers detected in eyes of infected and treated mice were 2-orders-of-magnitude lower than those corresponding to HSV-1 infected control animals. Mock-infected and treated mice did not reveal any corneal alteration due to the administration of the compound. Meliacine was found to exert a strong antiviral action on HSV-1-induced ocular disease in mice with no evidence of toxic effects.

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An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation

Alché

Barquero

Sanjuán

et al. 2002

Phytotherapy Research

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Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in the assembly of nucleocapsids. The present report provides additional evidence to elucidate the mode of action of MA against HSV-1. Time-of-addition experiments confirmed that MA affects a late event in the multiplication cycle of HSV-1. We showed that MA diminished the synthesis of viral DNA and inhibited the spread of infectious viral particles when HSV-1 that expresses beta-galactosidase activity was used. In addition, the lack of a protein with an apparent MW of 55 KD was detected in MA-treated cell extracts. Ultrastructural analysis of infected cells showed that, in the case of MA treatment, a large number of unenveloped nucleocapsids accumulated in the cytoplasm and a minor proportion of mature virus was found in cytoplasmic vesicles.These findings suggest that MA exerts an antiviral action on both the synthesis of viral DNA and the maturation and egress of HSV-1 during the infection of Vero cells.

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Virucidal activity in aqueous extracts obtained from Cedrela tubiflora leaves

Cited by 10 publications

References 4 publications

Block of vesicular stomatitis virus endocytic and exocytic pathways by 1-cinnamoyl-3,11-dihydroxymeliacarpin, a tetranortriterpenoid of natural origin

Block of vesicular stomatitis virus endocytic and exocytic pathways by 1-cinnamoyl-3,11-dihydroxymeliacarpin, a tetranortriterpenoid of natural origin

Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice

An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation

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