Virtual Electron Microscopy

Lee, King-Chung; Mak, L. S.

doi:10.1177/1066896911421173

Cited by 12 publications

(2 citation statements)

References 10 publications

(12 reference statements)

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“…3 ). Digital pathology could even allow the integration of electron microscopy,always considered a static technique for the need of physical preparation and analysis by the technician/pathologist, although the possibility to digitalize the ultrastructural pictures has been demonstrated [ 15 ]. Moreover, virtual microscopy could be used for the remote assessment of thick sections adequacy as well as for the selection of the region-of-interest to analyse.…”

Section: Discussionmentioning

confidence: 99%

Digital pathology for the routine diagnosis of renal diseases: a standard model

et al. 2020

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Whole-slide imaging and virtual microscopy are useful tools implemented in the routine pathology workflow in the last 10 years, allowing primary diagnosis or second-opinions (telepathology) and demonstrating a substantial role in multidisciplinary meetings and education. The regulatory approval of this technology led to the progressive digitalization of routine pathological practice. Previous experiences on renal biopsies stressed the need to create integrate networks to share cases for diagnostic and research purposes. In the current paper, we described a virtual lab studying the routine renal biopsies that have been collected from 14 different Italian Nephrology centers between January 2014 and December 2019. For each case, light microscopy (LM) and immunofluorescence (IF) have been processed, analysed and scanned. Additional pictures (eg. electron micrographs) along with the final encrypted report were uploaded on the web-based platform. The number and type of specimens processed for every technique, the provisional and final diagnosis, and the turnaround-time (TAT) have been recorded. Among 826 cases, 4.5% were second opinion biopsies and only 4% were suboptimal/inadequate for the diagnosis. Transmission electron microscopy (TEM) has been performed on 41% of cases, in 22% changing the final diagnosis, in the remaining 78% contributed to the better definition of the disease. For light microscopy and IF the median TAT was of 2 working days, with only 8.6% with a TAT longer than 5 days. For TEM, the average TAT was 26 days (IQR 6-64). In summary, we systematically reviewed the 6-years long nephropathological experience of an Italian renal pathology service, where digital pathology is a definitive standard of care for the routine diagnosis of glomerulonephritides.

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Section: Discussionmentioning

confidence: 99%

Digital pathology for the routine diagnosis of renal diseases: a standard model

et al. 2020

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“…Apart from this, WSI technology can be valuable for obtaining consultation on ultrathin sections from experts located in higher centers. 83…”

Section: Wsi and Immunohistochemistry And Electron Microscopymentioning

confidence: 99%

Whole Slide Imaging Technology and Its Applications: Current and Emerging Perspectives

Jain,

Patel,

Parwani

et al. 2023

Int J Surg Pathol

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Background. Whole slide imaging (WSI) represents a paradigm shift in pathology, serving as a necessary first step for a wide array of digital tools to enter the field. It utilizes virtual microscopy wherein glass slides are converted into digital slides and are viewed by pathologists by automated image analysis. Its impact on pathology workflow, reproducibility, dissemination of educational material, expansion of service to underprivileged areas, and institutional collaboration exemplifies a significant innovative movement. The recent US Food and Drug Administration approval to WSI for its use in primary surgical pathology diagnosis has opened opportunities for wider application of this technology in routine practice. Main Text. The ongoing technological advances in digital scanners, image visualization methods, and the integration of artificial intelligence-derived algorithms with these systems provide avenues to exploit its applications. Its benefits are innumerable such as ease of access through the internet, avoidance of physical storage space, and no risk of deterioration of staining quality or breakage of slides to name a few. Although the benefits of WSI to pathology practices are many, the complexities of implementation remain an obstacle to widespread adoption. Some barriers including the high cost, technical glitches, and most importantly professional hesitation to adopt a new technology have hindered its use in routine pathology. Conclusions. In this review, we summarize the technical aspects of WSI, its applications in diagnostic pathology, training, and research along with future perspectives. It also highlights improved understanding of the current challenges to implementation, as well as the benefits and successes of the technology. WSI provides a golden opportunity for pathologists to guide its evolution, standardization, and implementation to better acquaint them with the key aspects of this technology and its judicial use. Also, implementation of routine digital pathology is an extra step requiring resources which (currently) does not usually result increased efficiency or payment.

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Implementation of a virtual correlative light and transmission electron microscope

Voigt

Zuber

Gawatz³

et al. 2013

Microsc. Res. Tech.

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In the long run, the widespread use of slide scanners by pathologists requires an adaptation of teaching methods in histology and cytology in order to target these new possibilities of image processing and presentation via the internet. Accordingly, we were looking for a tool with the possibility to teach microscopic anatomy, histology, and cytology of tissue samples which would be able to combine image data from light and electron microscopes independently of microscope suppliers. With the example of a section through the villus of jejunum, we describe here how to process image data from light and electron microscopes in order to get one image-stack which allows a correlation of structures from the microscopic anatomic to the cytological level. With commercially available image-presentation software that we adapted to our needs, we present here a platform which allows for the presentation of this new but also of older material independently of microscope suppliers.

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Virtual Electron Microscopy

Cited by 12 publications

References 10 publications

Digital pathology for the routine diagnosis of renal diseases: a standard model

Digital pathology for the routine diagnosis of renal diseases: a standard model

Whole Slide Imaging Technology and Its Applications: Current and Emerging Perspectives

Implementation of a virtual correlative light and transmission electron microscope

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