Virome Identification and Characterization of Fusarium sacchari and F. andiyazi: Causative Agents of Pokkah Boeng Disease in Sugarcane

Abstract

Frontiers in Microbiology | www.frontiersin.org February 2020 | Volume 11 | Article 240 Yao et al. Virome Identification and Characterization of Fusarium spp.was observed and the characterization of the four-segment dsRNA chrysovirus was performed with aid of electron microscopy and analysis of the encapsidated RNAs. These findings provide insight into the diversity and spectrum of mycoviruses in PBD pathogens and should be useful for exploring agents to control the disease.

A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides

“…In F. andiyazi , FaMV1-162 infected strain Fa162 and a virus-free strain sample in the same area, Fa210, exhibited no significant differences in growth, conidiation, or virulence. These results are in agreement with those by Yao et al, [ 77 ] who reported that FaMV1 caused asymptomatic infection in F. andiyazi . In contrast, the F. verticillioides strain Sec505 infected with the novel mitovirus FvMV1 showed clear phenotypic differences with two virus-free F. verticillioides strains, FvM3125 and FvArv2300.…”

“…The advent and application of next generation sequencing techniques (e.g., RNA-seq and Metagenomic) have greatly increased the pace of mycovirus discovery in a wide diversity of fungi [ 5 , 6 ]. Up to date, mycoviruses had been identified in 16 different Fusarium species [ 52 , 58 , 77 , 85 ] including F. andiyazi , F. asiaticum , F. boothii , F. circinatum , F. coeruleum , F. globosum , F. graminearum , F. incarnatum , F. langsethiae , F. oxysporum , F. poae , F. pseudograminearum , F. sacchari , F. solani , F. culmorum and F. virguliforme . However, no mycovirus had been reported so far in F. verticillioides , the major causal agent of corn ear rot and fumonisin producer in the grains.…”

“…Therefore, according to ICTV rules, the mycovirus identified in F. andiyazi in this work represents a new strain of a previously identified mitovirus, which we have named Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). FaMV1 was identified in a recent study on sugarcane pathogens in China, by Yao and coworkers [ 77 ], together with a second mitovirus, Fusarium andiyazi mitovirus 2 (FaMV2). Interestingly, FaMV1 and FaMV1-162 also shared a 95.44% identity with a mitovirus identified in a different Fusarium species, Fusarium circinatum mitovirus 2-1 (FcMV2.1) isolated from the conifer pathogen F. circinatum in Spain [ 94 ].…”

“…Mitoviruses are widely distributed in nature [ 76 , 99 , 100 ], including a great diversity of fungal species [ 96 ]. Mitoviruses are the most common mycoviruses isolated from Fusarium species [ 52 , 77 , 96 ]. Infections caused by the majority of the mitoviruses reported so far are asymptomatic.…”

“…Phylogenetic insights were generated following the descriptions of Nibert et al [ 76 ] and Yao et al [ 77 ], with some modifications. Multiple sequence alignments of RdRp sequences were performed by optimized automatic adjustment using MAFTT version 7 [ 78 ] at (iterative refinement methods: L-INS-i strategy).…”