Virome analysis for identification of novel mammalian viruses in bats from Southeast China

Hu, Dan; Zhu, Chen; Wang, Yi; Ai, Lele; Yang, Lu; Ye, Fuqiang; Ding, Chenxi; Chen, Jiafeng; He, Bingfang; Zhu, Jingyuan; Qian, Hui; Xü, Wei; Feng, Youjun; Tan, Weilong; Wang, Changjun

doi:10.1038/s41598-017-11384-w

Cited by 55 publications

(63 citation statements)

References 40 publications

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“…Although most of the infected animals tested positive in the intestines (81.8%-9/11), in two animals, CoV was only detected in other organs (lungs and liver), raising the chances of viral detection. Other authors have also reported the presence of CoV RNA in tissues and secretions other than intestines or fecal samples, such as kidney, heart, lungs, and oropharyngeal swabs [25,55,56]. However, this is the first report of BtCoV detection in the liver.…”

Section: Discussionmentioning

confidence: 73%

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

et al. 2019

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Bats are flying mammals distributed worldwide known to host several types of Coronavirus (CoV). Since they were reported as the probable source of spillover of highly pathogenic CoV into the human population, investigating the circulation of this virus in bats around the world became of great importance. We analyzed samples from 103 bats from two distinct regions in Brazil. Coronavirus from the Alphacoronavirus genus was detected in 12 animals, 11 from São José do Rio Preto-SP region and 1 from Barreiras-BA region, resulting in a prevalence of 17.18% and 2.56% respectively. The virus was detected not only in intestines but also in lungs and liver. Phylogenetic analysis based on nsP12 genomic region suggests that the sequences group according to host family and sampling location. Studies on the circulation of these viruses in bats remain important to understand the ecology and evolutionary relationship of these pathogens.

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Section: Discussionmentioning

confidence: 73%

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

et al. 2019

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“…In Brazil, BtAdV has been detected in Desmodus rotundus, the most common vampire bat in Brazil [16]. Papillomaviruses (PVs), which are also dsDNA viruses, have also been reported in diverse bats species [17][18][19], but so far, they have not been reported in bats in Brazil. Parvoviruses, a family of single-stranded DNA (ssDNA) viruses that exhibits the highest mutation rate among DNA viruses [9] have been reported worldwide, including in Brazil [20] and have been shown to be related to human-infecting parvoviruses [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Detection of adenovirus, papillomavirus and parvovirus in Brazilian bats of the species Artibeus lituratus and Sturnira lilium

et al. 2019

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Bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. The diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. Therefore, the aim of this study was to detect fragments of viral genomes in bat samples. We performed high-throughput sequencing analysis and specific PCR and RT-PCR on pools of anal and oropharyngeal swabs from Artibeus lituratus and Sturnira lilium collected in southern Brazil. As a result, a member of the family Adenoviridae related to human adenovirus C was detected in anal swabs from S. lilium. In addition, we detected a papillomavirus in an anal swab from A. lituratus. Our analyses also allowed the detection of adenoviruses and parvoviruses in oropharyngeal swabs collected from A. lituratus. These results increase our knowledge about viral diversity and illustrate the importance of conducting virus surveillance in bats.

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“…RNA extraction and RT-PCR analysis. All samples were pooled and then underwent reverse transcription PCR analysis as previously described 36 . Briefly, each intestine sample (~0.1 g) was homogenized www.nature.com/scientificreports www.nature.com/scientificreports/ using a glass grinder with 10 volumes of SM buffer (50 mM Tris, 10 mM MgSO4, 0.1 M NaCl, pH7.5).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, several novel bat-associated viruses in southeast China were annotated through metagenomic sequencing technology 36 , including coronavirus, norovirus, adenovirus, bocavirus, astrovirus, circovirus, and adeno-associated viruses. To date, there have been no adeno-associated virus studies in the coastal areas of Southeast China.…”

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confidence: 99%

Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China

Zhu¹,

Wang²,

et al. 2020

Sci Rep

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Bats are associated with several important zoonotic viruses from different families. One example includes adeno-associated viruses (AAVs), that are extensively detected in several animals, especially primates. To understand AAVs distribution and genetic diversity in the coastal areas of Southeast China, a total of 415 intestine samples were mostly collected from two provinces of southeast China, i.e., Zhejiang and Fujian province. Intestine samples from five bat species were collected for AAVs detection. The average prevalence rate for AAV detection among these samples was 18.6% (77 positives out of 415 samples) and ranged from 11.8 to 28.9% between the five bat species. This suggests that AAVs are widely distributed in diverse bat populations in southeast coastal areas of China. Based on the genome sequence of bat adeno-associated virus-CXC1(BtAAV-CXC1) from one AAV-positive sample, the genetic diversity of the detected AAVs were assessed and analyzed. Phylogenetic analysis revealed that BtAAV-CXC1 was comparatively distant to other major AAVs from mammals and non-mammals, with only a 52.9~64.7% nucleotide identity. However, they were phylogenetically closer to Rhinolophus sinicus bat adeno-associated virus (Rs-BtAAV1), with a 74.5% nt similarity. Partial analysis of the rep and cap overlapping open reading frame (ORF) sequences from bat AAV samples revealed 48 partial rep sequences and 23 partial cap sequences from positive samples shared 86.9 to 100% and 72.3 to 98.8% nucleotide identities among themselves, respectively. This suggests that the detected AAVs had a distinctly high genetic diversity. These findings led us to conclude that diverse AAVs may be widely distributed in bat populations from the southeast regions of China.

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Virome analysis for identification of novel mammalian viruses in bats from Southeast China

Cited by 55 publications

References 40 publications

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

Detection of adenovirus, papillomavirus and parvovirus in Brazilian bats of the species Artibeus lituratus and Sturnira lilium

Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China

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