Virological Surveillance of Human Respiratory Syncytial Virus A and B at a Tertiary Hospital in Catalonia (Spain) During Five Consecutive Seasons (2013–2018)

Gimferrer, Laura; Vila, Juan J.; Piñana, María; Andrés, Cristina; Pendás, J.A. Rodrigo; Peremiquel-Trillas, Paula; Codina, María Gema; Martín, María del Carmen; Esperalba, Juliana; Fuentes, Francisco; Rubio, Susana; Campins-Martí, Magda; Pumarola, Tomás; Antón, Andrés

doi:10.2217/fmb-2018-0261

Cited by 25 publications

(33 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The remarkable number of tested samples (8212) during the 2017/18 season could be explained with the consciousness-raising of relevance of RSV disease among physicians. Therefore, from our point of view, the prevalence of this season (14.4%) might be the best representative in the country, being similar to findings from Senegal [33], Thailand [34], Spain [35] and different regions in India [36,37]. Nonetheless, a true comparison of RSV prevalence in different geographic areas is difficult to perform taking into account the differences in the study design and, even in our study, prevalence was wide-ranging according to the season.…”

Section: Discussionsupporting

confidence: 67%

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

View full text Add to dashboard Cite

A B S T R A C TIntroduction: Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary. Objective: The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period. Material and methods: Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0. Results: RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/ 16779), being higher (P < 0.0001) among children aged < 5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged < 6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9

show abstract

Section: Discussionsupporting

confidence: 67%

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

View full text Add to dashboard Cite

show abstract

“…14 These samples should be analyzed with PCR or immunofluorescent antigen detection of orthopneumovirus. 7 The IHC results in combination with the clinical signs, microscopic lesions, and the PCR data strongly support the conclusion that the gibbon group in our case became acutely ill as a result of human orthopneumovirus infection.…”

supporting

confidence: 86%

Acute human orthopneumovirus infection in a captive white-handed gibbon

et al. 2020

View full text Add to dashboard Cite

We report herein a fatal case of acute human orthopneumovirus (formerly respiratory syncytial virus) infection in a captive white-handed gibbon ( Hylobates lar). Other members of the housing group had mild respiratory signs. Gross examination revealed bilateral pulmonary congestion and froth in the bronchi. Microscopically, the lungs had lymphocytic, neutrophilic infiltration of the interstitium and alveolar walls. There was necrosis of terminal bronchiolar epithelium and terminal bronchioles, and surrounding alveoli contained necrotic and exfoliated epithelial cells admixed with histiocytes and syncytial cells. Additional lesions included nonsuppurative meningoencephalitis, and epidermal hyperkeratosis and hyperplasia with syncytial cell formation. PCR screening for 12 human respiratory viruses was positive for orthopneumovirus in multiple tissues, including lung, and immunohistochemical staining for human orthopneumovirus detected viral antigen within bronchial epithelial cells. IHC and PCR for measles virus on preserved sections were negative. White-handed gibbons have not been previously reported as hosts for human orthopneumovirus, an important respiratory pathogen of both primates and humans.

show abstract

“…A large study from South Africa (with 13,664 patients) between February 2009 to May 2012 showed that RSV was detected in 16% of the recruited subjects with more than 50% of them below 4 years of age [27]. G protein in the RSV is responsible for the virus attachment to the ciliated cells of the host airways and mediates virus adherence [28]. Therefore, G protein is the target for neutralizing antibodies but because of its high genetic variability and higher numbers of glycosylation sites it gives the virus the ability to evade the immune system and delay antigen recognition [29].…”

Section: Discussionmentioning

confidence: 99%

Dominance of the ON1 Genotype of RSV-A and BA9 Genotype of RSV-B in Respiratory Cases from Jeddah, Saudi Arabia

Al-Sharif

El‐Kafrawy

Yousef

et al. 2020

Genes

View full text Add to dashboard Cite

Human respiratory syncytial virus (HRSV) is a main cause of hospital admission for lower respiratory tract infection. In previous studies from Saudi Arabia, higher prevalence of the NA1 genotype in group A was observed from Riyadh and Taif. This study recruited respiratory cases from Jeddah during January to December, 2017. RSV represented 13.4% in the recruited cases with 64% of them belonging to group A and 36% to group B. All group A cases in this study were ON1 type characterized by duplication of 72 nucleotides, 24 amino acids in the C-terminal in the second hypervariable region of the G gene. In addition, for group B all of the cases were clustered under BA9, which had uniquely characterized as duplication of 60 nucleotides in the G protein. Our sequences showed similarity with earlier sequences from Saudi Arabia, Kuwait, Thailand, South Africa, Spain, the USA and Cyprus. Some amino acid substitutions in the investigated sequences would cause a change in potential O-glycosylation and N-glycosylation profiles from prototype ON1. The predominance of the ON1 and BA9 genotype of RSV-A in Jeddah compared to previous Saudi studies showing predominance of the NA1 genotype for group A. This difference in genotype prevalence could be due to fast spread of the ON1 genotype worldwide or due to the flux of travelers through Jeddah during hajj/umrah compared to Riyadh and Taif. This shift in genotype distribution requires continuous surveillance for genetic characterization of circulating respiratory infections including RSV. These findings may contribute to the understanding of RSV evolution and to the potential development of a vaccine against RSV.

show abstract

Virological Surveillance of Human Respiratory Syncytial Virus A and B at a Tertiary Hospital in Catalonia (Spain) During Five Consecutive Seasons (2013–2018)

Cited by 25 publications

References 38 publications

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Acute human orthopneumovirus infection in a captive white-handed gibbon

Dominance of the ON1 Genotype of RSV-A and BA9 Genotype of RSV-B in Respiratory Cases from Jeddah, Saudi Arabia

Contact Info

Product

Resources

About