Virological kinetics and immunological responses to a porcine reproductive and respiratory syndrome virus infection of pigs at different ages

Linden, I.F.A. van der; Voermans, J.J.M.; Linde-Bril, E M van der; Bianchi, A.T.J.; Steverink, P.J.G.M.

doi:10.1016/s0264-410x(02)00822-8

Cited by 48 publications

(35 citation statements)

References 14 publications

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“…Several factors, such as animal age and bacterial co-infection, can influence virus replication and clinical signs. Younger pigs (4-8 weeks of age) infected with PRRSV have been shown to exhibit a longer viraemia and higher excretion and replication rates in macrophages when compared to older (16-24 weeks of age) animals ( Van der Linden et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus

Štukelj¹,

Toplak²,

Svete³

2013

Polish Journal of Veterinary Sciences

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Porcine reproductive and respiratory syndrome (PRRS) has become one of the most economically important diseases for the swine industry worldwide. The objective of the study was to determine selected blood antioxidant enzymes (glutathione peroxidase (GPX), superoxide dismutase (SOD)), biochemical and haematological parameters in PRRS positive and negative pigs of three different categories, mainly to test oxidative stress hypothesis in pigs naturally infected with PRRS virus. Ninety PRRS positive and 90 PRRS negative pigs were included in the study. The presence of PRRS was confirmed by serological detection of antibodies against PRRS virus (PRRSV) and detection of PRRS viral RNA by RT-PCR. Pigs were further divided into three groups of 30: piglets just before weaning (weaners), fatteners and finishers. Blood samples for determining selected blood parameters were collected from the vena cava cranialis. Significantly (P < 0.05) higher activities of SOD in weaners and fatteners and of GPX in weaners were determined in PRRS positive pigs than in corresponding groups of PRRS negative pigs. In contrast, significantly (P < 0.05) lower GPX activity was observed in finishers of PRRS positive pigs than in the corresponding group of PRRS negative pigs. Concentrations of serum total protein in PRRS positive weaners and fatteners were significantly (P < 0.05) higher than those found in PRRS negative pigs. Leukopenia was observed in all three groups of PRRS positive pigs. It has been demonstrated, for the first time, that oxidative stress might be increased in PRRSV naturally infected pigs, especially in weaners.

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Section: Discussionmentioning

confidence: 99%

Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus

Štukelj¹,

Toplak²,

Svete³

2013

Polish Journal of Veterinary Sciences

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“…In this study, sensitivity estimates have been obtained for RT-PCR on different biological samples run individually, in pools of 3, and in pools of 5. It has been demonstrated that infection with highly virulent PRRSV isolates results in larger amounts of viral copies in serum than infection with attenuated isolates or isolates associated with low virulence. 2,11,24 Therefore, the decrease in sensitivity due to pooling could be influenced by the PRRSV isolate. Positive samples from animals infected with a low-virulent isolate would be more likely to test negative when pooled than those from animals infected with a highvirulent isolate.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Sensitivity of Reverse-Transcription Polymerase Chain Reaction to Detect Porcine Reproductive and Respiratory Syndrome Virus on Individual and Pooled Samples from Boars

Rovira

Clement²,

Christopher‐Hennings³

et al. 2007

J VET Diagn Invest

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Abstract. Boar studs are continuously monitored for the presence of porcine reproductive and respiratory syndrome virus (PRRSV) by testing different biological samples by reverse-transcription polymerase chain reaction (RT-PCR). In most cases, samples are run in pools, even though the impact of pooling on the sensitivity of RT-PCR is unknown. The objective of this study was to evaluate the feasibility of using PCR on pooled samples through the estimation of the sensitivity of RT-PCR on different biological samples run individually, in pools of 3 and in pools of 5. Twenty-nine boars were inoculated with a low virulent PRRSV isolate. Serum, blood swab, and semen samples were obtained from each boar every 2 to 3 days for 2 weeks. Each sample was tested by RT-PCR undiluted or diluted 1:3 and 1:5 with negative samples. Eleven of the 29 boars did not appear to get infected from the inoculum, as evidenced by no seroconversion 15 days after inoculation. Data from the other 18 boars showed that serum was the best sample to detect PRRSV during acute infection, with the blood swab sample performing almost as well. Semen samples failed to detect PRRSV infection in most of the cases. Pooling samples at pool sizes of 3 and 5 resulted in a decrease in the sensitivity of RT-PCR. Sensitivity was reduced by 6% and 8%, respectively, when serum or blood swab samples were run in pools of 5. The impact of pooling on the sensitivity of PCR was higher in samples taken during the beginning of the viremic period.

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“…The duration of viraemia varies depending on the PRRSV strain and on the age of the animal [20–23]. This general picture is similar in genotype 1 [22]. In both cases, several studies indicated that in general the period of viraemia may range from a few weeks (usually <4) in adults or grower-finishers to up to three months in very young piglets [23–26].…”

Section: Transmission Of Prrsvmentioning

confidence: 99%

Review on the transmission porcine reproductive and respiratory syndrome virus between pigs and farms and impact on vaccination

Pileri

Mateu

2016

Vet Res

147

132

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Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most costly diseases affecting intensive pig production worldwide. Control of PRRS is a complex issue and involves a combination of measures including monitoring, diagnosis, biosecurity, herd management, and immunization. In spite of the numerous studies dealing with PRRS virus epidemiology, transmission of the infection is still not fully understood. The present article reviews the current knowledge on PRRSV transmission between and within farm, and the impact of vaccination on virus transmission.

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Virological kinetics and immunological responses to a porcine reproductive and respiratory syndrome virus infection of pigs at different ages

Cited by 48 publications

References 14 publications

Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus

Blood antioxidant enzymes (SOD, GPX), biochemical and haematological parameters in pigs naturally infected with porcine reproductive and respiratory syndrome virus

Evaluation of the Sensitivity of Reverse-Transcription Polymerase Chain Reaction to Detect Porcine Reproductive and Respiratory Syndrome Virus on Individual and Pooled Samples from Boars

Review on the transmission porcine reproductive and respiratory syndrome virus between pigs and farms and impact on vaccination

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