Virological Characterization of Roof-Harvested Rainwater of Densely Urbanized Low-Income Region

Shubo, Tatsuo; Maranhão, Adriana Gonçalves; Ferreira, Fernando César; Fumian, Túlio Machado; Pimenta, Márcia Maria Araújo; Morgado, Cláudia do Rosário Vaz; Toze, Simon; Ahmed, Warish; Sidhu, J. P. S.; Miagostovich, Marize Pereira

doi:10.1007/s12560-021-09484-y

Cited by 7 publications

(3 citation statements)

References 49 publications

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“…In another study, using human coronavirus as a surrogate, it was demonstrated a slightly improvement in the recovery efficiency of SMF method, with results ranging from 9 to 4% using Vertrel™ treatment, and 6 to 2.6% without treatment [ 15 , 27 ]. The SMF method has been described as a low cost method being used to detect different enteric viruses in several aquatic matrices with satisfactory results without the use of Vertrel™ treatment [ 41 – 43 ]. Although with initial high cost relating to the ultracentrifuge acquisition, the ULT method proved to be efficient to detect SARS-CoV-2 [ 16 ] (Prado et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater

et al. 2023

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Wastewater-based epidemiology has been described as a valuable tool for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a community. However, there is no consensus on the best concentration method to allow reliable detection of SARS-CoV-2 in this matrix, considering different laboratory facilities. This study compares two viral concentration methods, ultracentrifugation (ULT) and skimmed-milk flocculation (SMF), for detecting SARS-CoV-2 in wastewater samples. The analytical sensitivity (limits of detection and quantification [LoD/LoQ]) of both methods was evaluated using a bovine respiratory syncytial virus (BRSV) as a surrogate. Three different approaches were conducted to establish LoD of each method based on the assays on the standard curve (ALoDsc), on the dilution of internal control (ALoDiC), and the processing steps (PLoD). For PLoD, ULT method had the lowest value (1.86 × 10 3 genome copy/microliter [GC/µL]) when compared to the SMF method (1.26 × 10 7 GC/µL). The LoQ determination showed a mean value of 1.55 × 10 5 GC/µL and 3.56 × 10 8 GC/µL to ULT and SMF, respectively. The detection of SARSCoV-2 in naturally contaminated wastewater revealed 100% (12/12) and 25% (3/12) of detection using ULT and SMF with quantification ranging from 5.2 to 7.2 log10 genome copy/liter (GC/L) and 5.06 to 5.46 log10 GC/L, respectively. The detection success rate of BRSV used as an internal control process was 100% (12/12) for ULT and 67% (8/12) for SMF, with an efficiency recovery rate ranging from 12 to 38% and 0.1 to 5%, respectively. Our data consolidates the importance of assessing the methods used; however, further analysis should be carried out to improve low-cost concentration methodologies, essential for use in low-income and developing countries.

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Section: Discussionmentioning

confidence: 99%

Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater

et al. 2023

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“…recovery used in this study was determined by the authors that designed this viral concentration method and it has been used by several authors worldwide (Assis et al, 2018;Borgmästars et al, 2021;Calgua et al, 2013;Gonzales-Gustavson et al, 2017;Shubo et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…We did not perform experiments in order to calculate the recovery efficiency of the protocol used in this study. However, the recovery efficiency used in this study was determined by the authors that designed this viral concentration method and it has been used by several authors worldwide (Assis et al, 2018; Borgmästars et al, 2021; Calgua et al, 2013; Gonzales‐Gustavson et al, 2017; Shubo et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the risk of infection of human rotavirus and astrovirus according to fishing and swimming activities at Las Cañas beach, Uruguay

Bortagaray

Gamazo

Castro

et al. 2022

Journal of Applied Microbiology

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Aims:To estimate the risk of human rotavirus (RV) and astrovirus (HAstV) infections for swimmers and fishers at Las Cañas beach, Uruguay. Methods and Results: Surface water samples were collected monthly for 1 year.The dose-response models used were β-Poisson and 1 F 1 hypergeometric for RV and HAstV, respectively. The probabilities of infection were calculated using a kernel density estimate to fitting the data and then sampling from this distribution (Monte Carlo simulation). The probability of RV infection for fishers was between 0 and 65% and for swimmers was between 0 and 50% (<18 years old) and between 0 and 38% (>18 years old). For HAstV, the probability of infection for fishers was between 0% and 45% and for swimmers was between 0 and 38% (<18 years old) and between 0 and 18% (>18 years old). Conclusions:This study suggests that fishers are at higher risk of infection for both viruses compared with swimmers mainly due to higher viral frequency and concentration at the site for fishing activities.

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Microbiological characterization of stormwater in a high-income neighborhood in Rio de Janeiro, Brazil

Shubo

Maranhão

Ferreira

et al. 2022

Environ Monit Assess

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Virological Characterization of Roof-Harvested Rainwater of Densely Urbanized Low-Income Region

Cited by 7 publications

References 49 publications

Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater

Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater

Comparison of the risk of infection of human rotavirus and astrovirus according to fishing and swimming activities at Las Cañas beach, Uruguay

Microbiological characterization of stormwater in a high-income neighborhood in Rio de Janeiro, Brazil

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