Viral Tools for Neural Circuit Tracing

Liu, Qing; Wu, Yang; Wang, Huadong; Fan, Jia; Xu, Fuqiang

doi:10.1007/s12264-022-00949-z

Cited by 25 publications

(19 citation statements)

References 89 publications

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“…Labeling methods of synaptic connections are more indicative of the strength of connections between different brain regions. 50 Therefore, this tracing method cannot reflect the real connection strength, but reveal the connection circuits between different regions or neurons to a certain extent.…”

Section: Discussionmentioning

confidence: 99%

Long-range connectome of pyramidal neurons in the sensorimotor cortex

Yao¹,

Tudi²,

Jiang³

et al. 2023

iScience

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Section: Discussionmentioning

confidence: 99%

Long-range connectome of pyramidal neurons in the sensorimotor cortex

Yao¹,

Tudi²,

Jiang³

et al. 2023

iScience

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“…Further improvements on MLS-SIM can benefit from advances in sample labeling 53,54 , hardware implementation, and algorithm development 47,48,55,56 . MLS-SIM can also be applied beyond neural imaging to image other cellular dynamics in the brain 57 , and other organs or tissues, such as beating hearts and breathing lungs, where sample motions have been prohibiting successful super-resolution imaging.…”

Section: Discussionmentioning

confidence: 99%

Super-Resolution Imaging of Fast Morphological Dynamics of Neurons in Behaving Animals

Zhang,

Bai,

Wang

et al. 2023

Preprint

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Neurons are best studied in their native working states in which their functional and morphological dynamics support animals' natural behaviors. Super-resolution microscopy can potentially reveal these dynamics in much higher details but has been challenging in behaving animals due to severe motion artifacts. Here, we report multiplexed line-scanning structured illumination microscopy, which could tolerate motions of up to 50 um/s while achieving 150 and 100 nm lateral resolutions in its linear and nonlinear forms respectively over large fields-of-view. We continuously imaged the dynamics of spinules in dendritic spines and axonal boutons volumetrically over thousands of frames and tens of minutes in mouse brains during sleep-wake cycles. Super-resolution imaging of axonal boutons revealed their prevalent spinule dynamics on a scale of seconds. Simultaneous two-color imaging further enabled analyses of the correlation between the spatial distributions of diverse PSD-95 clusters and the structural dynamics of dendrites in the brains of awake mice.

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“…Much more research is conducted with virus-based than nonviral tracers for neural circuit mapping, and there are excellent reviews on virus-based NaTTs. ,− Therefore, we highlight a very few major viral tracers and discuss their characteristics.…”

Section: Neuroanatomical Tract Tracer (Natt) Types and Characteristicsmentioning

confidence: 99%

“…Engineered viruses are capable of selectively tracing neuronal somas, mapping their axonal projections, and discriminating structurally and functionally connected polysynaptic neuronal circuit assemblies. We highlight only a few significant advances and refer readers to comprehensive reviews on engineered viruses for neural circuit mapping by others. ,− …”

Section: Advances In Neural Circuit Mappingmentioning

confidence: 99%

“…It may be valuable to the biomedical community to consider NaTTs in a broader context beyond their classical role for neural tracing. We take advantage of many excellent review articles on NaTTs, particularly those discussing the topic of viral vectors in mapping the structural and functional topography of the CNS and the peripheral nervous system (PNS), some published within the last three years. ,− After first summarizing the essential characteristics of the major viral and nonviral NaTT subclasses, we divide the subsequent sections according to their applications: neural circuit mapping, disease pathology and diagnosis, and drug and gene delivery. We hope that this Review will catalyze research discussions and collaborations among neuroscientists, biomedical researchers, and nanotechnologists that lead to new therapeutic options for the treatment of neurological diseases.…”

Section: Introductionmentioning

confidence: 99%

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Neuroanatomical Tract Tracers: Not Just for Neural Tracing Anymore

Wang

Clark

Mao

2023

ACS Appl. Bio Mater.

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Neuroanatomical tract tracers (NaTTs) have been used for neural circuit tracing for decades and now find recent applications in disease diagnosis and drug and gene delivery. In this Review, first the different subclasses of NaTTs including nonviral and viral types and their unique properties are discussed. The focus then is shifted to recent developments in improving the design and performance of NaTTs for neural circuit mapping, their role in disease diagnostics, and the emerging applications in drug and gene delivery targeted to the nervous system. In contrast to most molecular and biologic drugs that do not pass through the blood−brain barrier, NaTTs, including certain types of plant lectins, bacterial toxins, and some viruses, are readily taken up by nerve endings in mammalian muscle and efficiently transported within the central nervous system to the brain. Incorporating NaTTs into nanomedicines to bypass biological barriers and to deliver drugs to specific neurons thus presents an exciting direction and offers many possibilities for drug delivery. We hope that this Review will catalyze further discussions and collaborations among neuroscientists, biomedical researchers, and nanotechnologists that lead to innovative therapeutic options for treating neurological diseases.

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Viral Tools for Neural Circuit Tracing

Cited by 25 publications

References 89 publications

Long-range connectome of pyramidal neurons in the sensorimotor cortex

Long-range connectome of pyramidal neurons in the sensorimotor cortex

Super-Resolution Imaging of Fast Morphological Dynamics of Neurons in Behaving Animals

Neuroanatomical Tract Tracers: Not Just for Neural Tracing Anymore

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