Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo

Emmerich, Petra; Jakupi, Xhevat; Possel, Ronald von; Berisha, Lindita; Halili, B.; Günther, Stephan; Cadar, Dániel; Ahmeti, Salih; Schmidt‐Chanasit, Jonas

doi:10.1016/j.meegid.2018.07.010

“…The interactions between viruses and their respective invertebrate hosts should also be explored, as these viruses (as part of the tick microbiome) could have an impact on tick biology and its ability to transmit pathogens [24][25][26]. Finally, the evaluation of their potential pathogenicity-ability to be transmitted, virulence factors, etc.-for vertebrate hosts should be studied, as some of them, such as the JMTV, have already been involved in human diseases [12,[70][71][72]. Deciphering the complex mechanisms governing host-microbiome interactions could eventually help to find new and innovative ways to prevent and control tick-borne diseases in the Caribbean.…”

Section: Discussionmentioning

confidence: 99%

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard

¹

,

Temmam

²

,

Devillers

³

et al. 2020

Viruses

View full text Add to dashboard Cite

Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

show abstract

“…The JMTV group is a recently-discovered group of segmented ssRNA(+) viruses that infect different species of invertebrates, including insects, ticks and nematodes (1,4,8,11,(15)(16)(17). Moreover, some representatives of the new flavivirus-like viruses were detected in patients who had other severe infectious diseases (10), and these viruses might themselves cause illness in humans (5,8). There is still little information about the replication and overall coding strategy of this novel virus group.…”

Section: Discussionmentioning

confidence: 99%

“…The geographic distribution of the JMTV group is very wide, encompassing Asia (1, 4,8,9), Europe (9)(10)(11)(12), Central and South America (2,3,(12)(13)(14) and Africa (2,12). Virus RNAs of this group were detected not only in different species of insects, e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First isolation and characterisation of Alongshan virus in Russia

Kholodilov

¹

,

Litov

²

,

Klimentov

³

et al. 2019

Preprint

View full text Add to dashboard Cite

AbstractIn recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmen tick virus (JMTV) group holds a special place among flavi-like viruses because, in contrast to the “classic” flaviviruses and other flavi-like viruses, they have a segmented ssRNA(+) genome. Two segments of the JMTV genome have homology with regions of the flavivirus genome encoding polymerase and helicase-protease proteins. JMTVs have several open reading frames (ORF) in segments encoding glycoprotein(s) and capsid protein and these ORF are specific only to them. JMTVs greatly differ in virion size.We isolated three strains of Alongshan virus (ALSV), which is a representative of the JMTV group, from adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMTV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream ORF in the glycoprotein-coding segment of ALSV and other members of the JMTV group.Significance StatementWe isolated three strains of Alongshan virus (ALSV) from adult Ixodes persulcatus ticks from two geographically separate areas of Russia in the Ixodes ricinus tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Our study confirmed the value of tick cell lines in virus isolation and maintenance of persistent infection. The majority of virions of the ALSV strain Miass527 were enveloped spherical particles with a diameter of 40.5±3.7 nm. We found evidence for the existence of a novel upstream ORF in the glycoprotein-coding segment of ALSV and other members of the Jingmen tick virus group.

show abstract

“…Finally, the evaluation of their potential pathogenicity -ability to be transmitted, virulence factors, etc. -for vertebrate hosts should be studied, as some of them, such as the JMTV, have already been involved in human diseases [12,[70][71][72]. Deciphering the complex mechanisms governing hostmicrobiome interactions could eventually help to find new and innovative ways to prevent and control tick-borne diseases in the Caribbean.…”

Section: Discussionmentioning

confidence: 99%

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard¹,

Temmam²,

Devillers³

et al. 2019

Preprint

0

View full text Add to dashboard Cite

Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

show abstract

Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo

Cited by 75 publications

References 10 publications

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

First isolation and characterisation of Alongshan virus in Russia

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Contact Info

Product

Resources

About