Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait

Al‐Awadhi, Rana; Chehadeh, Wassim; Al-Jassar, Waleed; Al-Harmi, Jehad; Al-Saleh, Eyad; Kapila, Kusum

doi:10.3855/jidc.2748

Cited by 13 publications

(12 citation statements)

References 25 publications

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“…High risk HPVs in the current study were observed in 16.1% of NILM Pap smears, which fall near the lower third of the worldwide (0%-60%) range rates (Clifford et al, 2005;Bosch et al, 2008;Pity et al, 2012;Al-Awadhi et al, 2013;Pity 2013;Faik et al, 2015;Donkoh, 2016). Unmasking the burden of HR-HPV in NILM smears is notorious because of their effective ability to transform normal cell phenotype into a malignant one.…”

Section: Mixed Hpv Infectionsupporting

confidence: 47%

Human Papillomavirus Genotyping among Different Cervical Smears in Duhok/Iraq

Pity

Abdo

Goreal

2019

Asian Pac J Cancer Prev

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Background/Objective:Cervical cancer ranks the second among the most common gynecologic cancers. This study was established to determine the distribution of cervical HPV genotypes among different Pap readings in Duhok/Iraq. Methods:Between January and September-2016, HPV-DNA was tested in 64 women. Genotyping was carried out by the hybridization reverse blot technique. Cervical smears were taken, performed by ThinPrep technique and stained by Pap stain. Results:Twenty six (40.6%) cases were positive for HPV, 12 (46.2%) in normal and 14 (53.8%) in abnormal Pap smears. 39 (19 high-risk and 16 low-risk) genotypes were identified. The high risk group comprised 6 HPV16, 4 HPV18, 2 HPV66, 2 HPV52, 2 HPV39, 1 HPV56, 1 HPV31 and 1 HPV45. The 16 low risk strains encompassed 4 HPV6 strains, 4 HPV71, 2 HPV54 and 2 HPV83, HPV11, HPV61 HPV84, and HPV62. Mixed infections were described in 4 women (6.25%), limited to the NILM, ASC-US and LSIL smears. They included variable admixtures of 7 high risk genotypes, HPV39 (both copies), HPV66 (both copies), HPV52, HPV31, HPV45 and 6 low risk strains: HPV83, HPV6, HPV11, HPV54, HPV62 and HPV71. Conclusions:The higher frequency of HR-HPV than the LR-HPV with identification of 4 mixed cases indicates that our women are at risk of developing cervical cancer. Detection of HR-HPV in NILM and ASC-US smears with restriction of some strains to these 2 categories highlights the great value of HPV genotyping as a surrogate test to pick up unscreened women at risk of developing cervical malignancy particularly when a proper screening program is absent.

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Section: Mixed Hpv Infectionsupporting

confidence: 47%

Human Papillomavirus Genotyping among Different Cervical Smears in Duhok/Iraq

Pity

Abdo

Goreal

2019

Asian Pac J Cancer Prev

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“…Although higher viral loads may result from an increased rate of viral replication and may sustain viral persistence [Yoshida et al, 2008], this study demonstrated that the viral loads of HPV 52 and 16 at entry were not associated with the degree of cytologic abnormalities, supporting the idea that HPV DNA load seems to predict the risk of developing cervical carcinoma before any cytological alterations are visible . This result is not in accordance with previous studies [Carcopino et al, 2012;Al-Awadhi et al, 2013], and this discrepancy may reflect the small sample size used in the current study. Further studies using a greater number of patients are required to establish the reason for this discrepancy.…”

Section: Discussioncontrasting

confidence: 99%

Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix

Hamaguchi

Miura

Abe

et al. 2013

Journal of Medical Virology

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The aim of this study was to investigate the relationship between viral load in single human papillomavirus (HPV) 16 or 52 persistent infection and the progression of later cytopathological findings in the uterine cervix. Cervical cytology and HPV genotyping tests were repeated within 3-6 months in 305 women with oncogenic HPV. Twenty-four cases of single HPV 52 persistent infection and 24 cases of single HPV 16 persistent infection were identified. Cases with later cytopathological findings showing progression were defined as the progression group, while those with no change or regression were the non-progression group. Relative HPV DNA loads were determined by quantitative real-time polymerase chain reaction and expressed relative to human albumin (ALB) DNA. Differences between the two groups were evaluated. The median relative HPV 52 DNA load was 2.211 in the progression group and 0.022 in the non-progression group (Mann-Whitney U-test, P = 0.003). The median relative HPV 16 DNA load was 4.206 in the progression group and 0.103 in the non-progression group (P = 0.001). HPV 52 and 16 DNA loads assessed by quantitative real-time methods may be useful short-term markers for identifying women at high risk for progression of cervical cytological pathology.

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“…In this general screening, we found only a few LGSIL and HGSIL cases. As is the situation in Kuwait, viral load and higher numbers of samples might reveal a situation with a different picture [27]. We are in the process of starting a prospective study to measure E6/E7 mRNA [28] in fresh cervical specimens and then follow those positive cases to explore the progress to cervical carcinoma.…”

Section: Discussionmentioning

confidence: 99%