Viral interference between H9N2-low pathogenic avian influenza virus and avian infectious bronchitis virus vaccine strain H120 in vivo

Aouini, Rim; Laamiri, Nacira; Nsiri, Jihene; SAID, S BEN; Miled, Khaled; Ahmed, Rejab; Ghram, Abdeljelil

doi:10.1016/j.cimid.2019.06.004

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…Chicken embryos that die within 24 h were considered to be non-specific deaths and were discarded. (7) Chicken embryo harvesting: After culturing, chicken embryos were stored at 4 • C overnight before harvesting. (8) A sterile10 mL tube labeled with the corresponding chicken embryo number was used for harvesting the embryos.…”

Section: Recovery Of H9n2 Virusmentioning

confidence: 99%

“…In recent years, the number of cases of avian influenza virus in China has gradually increased [ 3 , 4 , 5 , 6 ]. The H9N2 subtype avian influenza virus is a low pathogenic avian influenza virus [ 7 ]. After infection, it generally does not cause disease or death of poultry, but can reduce the production performance of sick poultry [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Glycine Nano-Selenium Enhances Immunoglobulin and Cytokine Production in Mice Immunized with H9N2 Avian Influenza Virus Vaccine

Ren

Okyere

Zhang

et al. 2022

IJMS

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This study was performed to investigate the immune enhancement effect of glycine nano-selenium, a microelement on H9N2 avian influenza virus vaccine (H9N2 AIV vaccine) in mice. Fifty (50) Specific Pathogen Free Kunming mice aged 4–6 weeks (18–20 g Body weight) were randomly divided into five groups: control normal group, which received no immunization + 0.5 mL 0.9% normal saline, positive control group, which received H9N2 AIV vaccine + 0.5 mL 0.9% normal saline, 0.25 mg/kg selenium group, which received H9N2 AIV vaccine + 0.5 mL 0.25 mg/kg selenium solution, 0.5 mg/kg selenium group, which received H9N2 AIV vaccine + 0.5 mL 0.5 mg/kg selenium solution, and 1 mg/kg selenium group, which received H9N2 AIV vaccine + 0.5 mL 1 mg/kg selenium solution. Hematoxylin and eosin staining, enzyme linked immunosorbent assay (ELISA), and quantitative real time polymerase chain reaction (qRT-PCR) methods were used to investigate the pathological changes, immunoglobulin levels, and cytokine gene expressions in this study. The results showed that all tested doses (0.25 mg/kg, 0.5 mg/kg and 1.00 mg/kg) of glycine nano-selenium did not lead to poisoning in mice. In addition, when compared to the positive control group, glycine nano-selenium increased the immunoglobin indexes (IgA, IgG, IgM and AIV-H9 IgG in serum) as well as the mRNA levels of IL-1β, IL-6 and INF-γ in the liver, lungs, and spleen (p < 0.05). In summary, glycine nano-selenium could enhance the efficacy of avian influenza vaccine.

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Section: Recovery Of H9n2 Virusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Glycine Nano-Selenium Enhances Immunoglobulin and Cytokine Production in Mice Immunized with H9N2 Avian Influenza Virus Vaccine

Ren

Okyere

Zhang

et al. 2022

IJMS

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“…Reducing surface receptor molecules is the main antiviral mechanism, but it works differently for each virus. It may be controlled by interactions between cytokines and interferons or sialidase activity in the host [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in five governorates in the Nile Delta, Egypt, from January 2022 to October 2022

El-Shemy,

Amer,

Hassan

et al. 2024

Vet World

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Background and Aim: Respiratory viral infections significantly negatively impact animal welfare and have significant financial implications in the poultry industry. This study aimed to determine the frequency of the most economically relevant respiratory viruses that circulated in Egyptian chicken flocks in 2022. Materials and Methods: Chickens from 359 broiler flocks in five different Egyptian governorates in the Nile Delta (Beheira, Gharbia, Giza, Monufiya, and Qalyoubia) at marketing time (33–38 days of age) were used in this study. Combined oropharyngeal and cloacal swabs and tissue samples were collected from clinically diseased or freshly dead birds suffering from respiratory disease. Avian influenza (AI)-H5, AI-H9, Newcastle disease (ND), and infectious bronchitis virus (IBV) were analyzed by reverse transcriptase polymerase chain reaction. Results: Of the 359 flocks examined, 293 tested positive, whereas 66 were completely negative for the four viruses evaluated, with the highest positive results in Beheira. Out of 293 positive flocks, 211 were positive for a single virus, with Beheira having the highest rate, followed by Qalyoubia, Giza, and Monufiya. ND virus (NDV) was found to be the highest across all governorates, followed by IBV, AI-H9, and AI-H5. A double infection was detected in 73 flocks with either H9 or ND, or both H9 and IB could coinfect each other. The most common viral coinfections were H9 + IB, ND + IB, and ND + H9. Giza had the highest prevalence of ND + H9, H9 + IB, and ND + IB coinfection in the governorates, followed by Monufiya and Beheira. Only six out of 359 flocks were tribally infected with ND + H9 + IB in Giza, Monufiya, and Beheira governorates. On the basis of the number of flocks and the month of the year, July had the lowest number of flocks (23), while September and October had the highest number (48 flocks). Positive flock numbers were highest in October and lowest in January. Conclusion: From January to October 2022, prevalent respiratory viral infections (H5N1, NDV, H9N2, and IBV) were detected in broiler chickens across the Delta area governorate, according to the findings of the present study. In addition, IBV and H9, either alone or in combination, significantly contributed to the respiratory infection observed in broiler chickens. Regardless of the type and origin of the vaccine used, it is not possible to protect broiler chickens from the development of the infection and the subsequent dissemination of the virus into the poultry environment. In the presence of face-infectious field virus mutations, poultry vaccinations must be regularly reviewed and updated, and poultry farms must take further biosecurity measures. Keywords: avian influenza virus, chicken, Egypt, infectious bronchitis, Newcastle disease, reverse transcriptase polymerase chain reaction, subtype H5 and H9.

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“…Instead of a virus-speci c mechanism primarily based on competition for the same susceptible cells, as concluded in one of the previous studies, these observations might indicate an underlying epithelial antiviral mechanism-based surface receptor molecule reduction that varies for different viruses. This mechanism could potentially be regulated by an interaction between different cytokine and interferon types or result from speci cally induced host sialidase activity (35).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in six governorates in the Nile Delta, Egypt, January to October 2022

El-Shemy¹,

Amer

Hassan

et al. 2023

Preprint

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Background and Aim: Respiratory viral infections have a considerable detrimental impact on animal welfare as well as significant financial ramifications in the poultry industry. Avian influenza virus (AIV) subtypes H5 and H9, Newcastle disease (ND), and infectious bronchitis (IB) are the most economically significant illnesses impacting the poultry sector worldwide, including Egypt. From January to October 2022, this study examined the presence of respiratory viral infections (AI-H5, AI-H9, ND, and IB) in 359 flocks of broiler chickens (33–38 days) in six Egyptian governorates (Beheira, Gharbia, Giza, Monufiya and Qalyoubia). Results Out of 359 flocks examined, 293 tested positive, whereas 66 tested fully negative for the four viruses tested with the highest positive results in Beheira. Out of 293 positive flocks, 211 were positive to a single virus with Beheira having the highest rate, followed by Qalyoubia, Giza, and Monufiya. NDV was found to be the highest across all governorates, followed by IBV, AI-H9, and AI-H5. Double infection was detected in 73 flocks with either H9 or ND or both H9 and IB would co-infect one another. The most common viral co-infection was H9 + IB, ND + IB, and ND + H9. Giza had the greatest prevalence of co-infection with ND + H9, H9 + IB, and ND + IB in the governorates, followed by Monufiya and El Buhyera. Only 6 out of 359 flocks were tribally infected with ND + H9 + IB in three governorates: Giza, Monufiya, and Beheira. According to the number of flocks and the month of the year, July had the fewest tested flocks (23) and both September and October had the most (48 flocks). The positive flocks were highest in October and lowest in January. Conclusion The results revealed that IBV and H9 as a single or a mixed infection had a great role in the respiratory infection in broiler. The used vaccine (regardless their origin and type) is not able to protect broiler chickens from developing infection and shedding of virus to the poultry environment. Therefore, poultry vaccines need regular evaluation, renovation in face infective field virus mutants and also, poultry farms must be adopting more biosecurity measures.

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Viral interference between H9N2-low pathogenic avian influenza virus and avian infectious bronchitis virus vaccine strain H120 in vivo

Cited by 6 publications

References 36 publications

Glycine Nano-Selenium Enhances Immunoglobulin and Cytokine Production in Mice Immunized with H9N2 Avian Influenza Virus Vaccine

Glycine Nano-Selenium Enhances Immunoglobulin and Cytokine Production in Mice Immunized with H9N2 Avian Influenza Virus Vaccine

Epidemiological distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in five governorates in the Nile Delta, Egypt, from January 2022 to October 2022

Epidemiological Distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in six governorates in the Nile Delta, Egypt, January to October 2022

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