1994
DOI: 10.1128/jvi.68.8.4707-4715.1994
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Viral induction of the human beta interferon promoter: modulation of transcription by NF-kappa B/rel proteins and interferon regulatory factors

Abstract: Multiple regulatory domains within the-100 region of the beta interferon (IFN-P) promoter control the inducible response of the IFN gene to virus infection. In this study, we demonstrate that the formation of NF-KB-specific complexes on the positive regulatory domain II (PRDII) precedes the onset of detectable IFN-I transcription in Sendai virus-infected cells. By using NF-KB subunit-specific antibodies, a temporal shift in the composition of NF-KB subunits in association with the PRDII domain is detected as a… Show more

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Cited by 84 publications
(22 citation statements)
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“…Although our studies are the first to describe RelA activation by a mucosa-restricted virus, NF-B activation has been reported in myeloid cells as a consequence of chronic lentivirus infection (45,47), acute Sendai virus infection of fibroblastic and renal carcinoma cells (22), and picornavirus infection of alveolar epithelial cells (60). Lentivirus infection of myeloid cells activates NF-B chronically; given the ability of lentivirus to stimulate cytokine production, many of which can themselves stimulate NF-B, it is difficult to determine whether RelA activation is a direct effect of viral replication or a secondary paracrine phenomenon.…”
Section: An Inflammatory Gene Network Controlled By Nf-bmentioning
confidence: 75%
“…Although our studies are the first to describe RelA activation by a mucosa-restricted virus, NF-B activation has been reported in myeloid cells as a consequence of chronic lentivirus infection (45,47), acute Sendai virus infection of fibroblastic and renal carcinoma cells (22), and picornavirus infection of alveolar epithelial cells (60). Lentivirus infection of myeloid cells activates NF-B chronically; given the ability of lentivirus to stimulate cytokine production, many of which can themselves stimulate NF-B, it is difficult to determine whether RelA activation is a direct effect of viral replication or a secondary paracrine phenomenon.…”
Section: An Inflammatory Gene Network Controlled By Nf-bmentioning
confidence: 75%
“…The cDNA probes were labeled with [a-32 P]dCTP (NEN) using random nonamer priming (Pharmacia Biotech). The p50 cDNA probe was a 1.5-kb EcoRI insert, p65 was a 0.95-kb EcoRI insert, IkBa was a 1.2-kb EcoRI insert (gifts of John Hiscott, McGill University) (28), and an 18S rRNA probe was used (Ambion, 18S Decatemplate). The filters were hybridized with the [a-32 P]dCTP-labeled cDNA probes at 42°C for 2 hr in Rapid-Hyb Buffer (Amersham) and washed following the manufacturer's recommendation.…”
Section: Rna Isolation Cdna Probes and Northern Blot Analysismentioning
confidence: 99%
“…Transfections and CAT reporter gene assays. Subconfluent 293 cells, CMVt-rtTA 293 cells, or CMVt-rtTA-IB-expressing 293 cells were transfected with the IFN-␤-CAT reporter plasmid, by the calcium phosphate coprecipitation method (17). All of the transfections contained equivalent amounts of DNA standardized with the CMV-B1 vector.…”
Section: Generation Of Plasmidsmentioning
confidence: 99%