Viral Identification by Scanning Electron Microscopy of Preparations Stained with Fluorescein-Labeled Antibody

Springer, Erik; Riggs, John L.; Hackett, Adeline J.

doi:10.1128/jvi.14.6.1623-1626.1974

Cited by 7 publications

(1 citation statement)

References 6 publications

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“…During the preparation of this report at least four papers have been published on SEM studies of virus particles at cell membranes (7,9,10,12). The importance and advantages of the SEM have been pointed out by these authors.…”

Section: Discussionmentioning

confidence: 99%

Studies on the budding process of a temperature-sensitive mutant of murine leukemia virus with a scanning electron microscope

Wong¹,

MacLeod²

1975

J Virol

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The scanning electron microscope was used to study the budding process of the wild-type Moloney murine leukemia virus and one of its temperature-sensitive mutants, designated ts 3. A considerably larger number of budding particles was observed on TB cells infected with ts 3 at the nonpermissive temperature (39 C) than at the permissive temperature (34 C). No apparent difference was noted between the number of particles on ts 3-infected cells at (34 C) and wild-typeinfected cells at 34 or 39 C. Virions were detected at the cell membrane of ts 3-infected cells at 39 C as early as 8 h postinfection. Virion density increased progressively up to 48 h after which no increase was observed. An average of 1,600 virus particles was observed at the cell surface at the peak of virus production. The distribution of these on the cell membrane appeared to be random. The maximum proportion of the cell surface occupied by the viral particles did not exceed 10%. After temperature shift from 39 to 34 C, approximately 90% of the particles had dissociated from the cell membrane within 1 h.

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Section: Discussionmentioning

confidence: 99%