Viral contamination of bovine foetal serum and cell cultures

Nuttall, Patricia A.; Luther, Peter; Stott, E. J.

doi:10.1038/266835a0

Cited by 92 publications

(43 citation statements)

References 6 publications

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“…The presence of foreign BVDV strains in permanent cell lines or in foetal calf serum batches may cause serious problems during the development and/or production of the live vaccines. If such cells or serum are used in vaccine production, the vaccine can be contaminated with ncp BVDV and become a source of spread of BVDV infections [26]. A further possible scenario is the accidental pickup of a cp strain and the continued production of the vaccine from this foreign cp virus.…”

Section: Discussionmentioning

confidence: 99%

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Bálint¹,

Baule

Pálfi³

et al. 2005

Vet. Res.

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-A live bovine viral diarrhea (BVDV) vaccine, marketed as a derivate of the Oregon C24V strain, was used between the end of the 1960s and the beginning of the 1990s in Central Europe. Since laboratory investigations of mucosal disease cases in vaccinated animals suggested recombinations between the vaccine and wild type variants of BVDV, and recombinational nucleotide sequences seemed distinct from BVDV Oregon C24V, the aim of the present retrospective study was to analyze the genomes of pre-registration (termed here BVDV-Xpre) and of marketed (BVDV-X) batches of the vaccine. The results of the complete genome analysis of BVDV-Xpre confirmed that the original virus strain used at the start of the vaccine production was Oregon C24V. Surprisingly, the analysis of the complete nucleotide sequence of the BVDV-X marketed vaccine revealed that this strain belongs to the BVDV 1b subgroup, with a 93.7% nucleotide sequence homology to BVDV reference strain Osloss. The homology to BVDV Oregon C24V was significantly lower (77.4%), and a thorough sequence scanning showed that the genome of BVDV-X had not derived from Oregon C24V. These data indicate the very likely scenario that a strain different to Oregon C24V was picked up during the in vitro or in vivo passages for vaccine development. Despite of the virus-switch, the BVDV-X vaccine continuously maintained its innocuity and efficacy, as proven by the regular quality testing data, and the presence of the foreign virus remained unnoticed over many years. The results of this work emphasize that the contamination of commercially available live vaccines with exogenous BVDV strains is a real risk factor, and a unequivocal analysis, including molecular methods, is needed to verify their authenticity. bovine viral diarrhea virus / vaccine / strain switch / control

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Section: Discussionmentioning

confidence: 99%

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Bálint¹,

Baule

Pálfi³

et al. 2005

Vet. Res.

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“…However, the drawback of FBS is that it involves a microbial contamination risk, in particular viral contamination, since it might be obtained from infected animals. A number of studies highlight the presence of fetal-borne pestivirus contamination in commercial fetal bovine serum (Nuttall et al, 1977;Makoschey et al, 2003). Pestivirus contamination is also a current problem in our laboratory, which requires testing of each single purchased bottle of FBS.…”

Section: Introductionmentioning

confidence: 99%

Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum

Ceylan

Severcan

Özkul

et al. 2012

Veterinary Microbiology

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The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220MPa pressure for 5min at 25°C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands in the finger-print region (1500-900cm -1) originating mainly from lipids which are thought to result from changes in the lipoprotein structure. The virus strain lost its infectivity completely after 220MPa HHP treatments. These results indicate that HHP can be successfully used for inactivation of pestiviruses while leaving structural and functional properties of serum and serum products unaffected. © 2011 Elsevier B.V

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“…9 Dogs are reportedly more susceptible to infection with M. tuberculosis and M. bovis, 2,5 and relatively resistant to mycobacteria belonging to the Mycobacterium avium complex (MAC), previously known as M. avium-M. intracellulare 1,3,5 Attempts to experimentally infect dogs by feeding them large numbers of M. avium organisms have demonstrated that healthy dogs have considerable innate resistance to infection from oral exposure. 3 Still, there have been sporadic reports in recent years of dogs infected with MAC and other nontuberculous or atypical mycobacterial strains.…”

mentioning

confidence: 99%

“…Cell cultures can be contaminated with ncBVDV if the bovine fetal or calf serum, a necessary component of cell culture media, 5,6,7,9,10 contains ncBVDV. Contamination with ncBVDV can also occur in a laboratory by the careless handling of clinical specimens infected with BVDV.…”

mentioning

confidence: 99%

A Method to Detect Bovine Viral Diarrhea Virus Contamination in Cell Cultures using Immunoperoxidase Staining

Castro

Stoffregen²,

Brigman³

et al. 1997

J VET Diagn Invest

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Certain tests in veterinary diagnostic laboratories are dependent on the use of cell cultures free of contamination with noncytopathic (nc) bovine viral diarrhea virus (BVDV). Such tests include virus and chlamydia isolations, serum neutralization assays, and the microplate virus isolation technique for detection of BVDV in persistently infected animals. 1,3 Cells infected with ncBVDV appear morphologically

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Viral contamination of bovine foetal serum and cell cultures

Cited by 92 publications

References 6 publications

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum

A Method to Detect Bovine Viral Diarrhea Virus Contamination in Cell Cultures using Immunoperoxidase Staining

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