Viral and non-viral induced fusion of pronase-digested human erythrocyte ghosts

Laster, Yehudith; Lalazar, Avraham; Loyter, Abraham

doi:10.1016/0005-2736(89)90006-0

Cited by 14 publications

(2 citation statements)

References 23 publications

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“…Pronaseand/or neuraminidase-treated ROV were obtained from erythrocyte membranes treated with Pronase (0.5 mg/mL, 30 min at 37 °C) and/or neuraminidase [30 milliunits for 1 mL of 40%, (v/v) blood, 1 h at 37 °C], respectively (Laster et al, 1979;Lalazar et al, 1977).…”

Section: Methodsmentioning

confidence: 99%

“…Receptors for Sendai virus have been removed from the erythrocyte membranes by treatment with neuraminidase and Pronase. It has been well established (Laster et al, 1979;Wolf et al, 1980;Nussbaum et al, 1984) that Sendai virions neither bind to nor fuse with such treated erythrocytes. Intracellular organelles can also serve as a convenient model system for biological membranes which naturally never encounter or fuse with enveloped animal virions.…”

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confidence: 99%

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Osmotic swelling allows fusion of Sendai virions with membranes of desialized erythrocytes and chromaffin granules

Citovsky¹,

Laster²,

Schuldiner³

et al. 1987

Biochemistry

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Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octadecyl Rhodamine B chloride) virions, with right-side-out erythrocyte vesicles (ROV) or with chromaffin granule membrane vesicles (CGMV), resulted in fluorescence dequenching. Fusion of Sendai virions with virus receptor depleted ROV was observed only under hypotonic conditions. Fusion with virus receptor depleted ROV required the presence of the two viral envelope glycoproteins, namely, the HN and F polypeptides. A 3-fold increase in the degree of fluorescence dequenching (virus-membrane fusion) was also obtained upon incubation of Sendai virions with CGMV in medium of low osmotic strength. This increase was not observed with inactivated, unfusogenic Sendai virions. The results of the present work demonstrate that, under hypotonic conditions, fusion between Sendai virions and biological membranes does not require the presence of specific receptors. Such fusion is characterized by the same features as fusion with and infection by Sendai virions of living cultured cells.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%