Viper toxins affect membrane characteristics of human erythrocytes

Doltchinkova, Virjinia; Stoylov, Stoyl P.; Angelova, Plamena R.

doi:10.1016/j.bpc.2020.106532

Cited by 6 publications

(8 citation statements)

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“…The samples were aliquoted into Eppendorf tubes and homogenized. Tubes (Heparin Disposable Pipettes, containing 50 microliters, 67 mm length, made in USA, by Drumond Scientific Company, distributed by Rudolph GRAVE AB Stockholm, Sweden) of precise bore glass were filled with the human erythrocyte suspension, one end was sealed and the tubes were centrifuged for the constant packing of cells [ 9 ]. The relative heights of the packed cell column and the total fluid were measured using a special optical device.…”

Section: Methodsmentioning

confidence: 99%

“…The biophysical state of the erythrocyte membrane is directly related to the surface charge, the presence of which can be calculated based on the electrophoretic mobility and zeta potential of the cell [ 8 , 9 ]. The registration of the movement of blood cells in a constant electric field under physiological conditions of the suspension medium allows an evaluation of the functions of the erythrocyte membrane.…”

Section: Introductionmentioning

confidence: 99%

“…When various chemical agents are applied to the erythrocyte membranes, which are released during inflammation and/or in the presence of pooled immunoglobulin G, as well as the membrane-disruptive melittin in the presence of IgG, molecular changes to the plasma membrane of the cell are observed. Erythrocyte membranes are the immediate target of the damaging action of a number of factors in the pathological process of the erythrocyte life cycle together with the initiation of lipid peroxidation as a universal mechanism of cell damage [ 9 , 12 ]. The application of additional biophysical studies on erythrocyte membranes after exposure to pooled IgG, hematin and melittin could be a contribution to clarifying the mechanism of membrane alteration.…”

Section: Introductionmentioning

confidence: 99%

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Erythrocyte Membrane Biophysical Changes Mediated by Pooled Immunoglobulin G and Hematin: Electrokinetic and Lipid Peroxidation Studies

et al. 2023

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Pooled Immunoglobulin G (IgG), hematin and the membrane-disruptive amphipathic peptide melittin have received attention as powerful biomacromolecules for biomedical and pharmacology applications. Their action on surface properties, oxidation status and epifluorescence properties measured in vitro provide useful information about the functional activity of upper biomacromolecules in erythrocytes in vivo. The hemolysis of erythrocyte membranes, as well as changes in hematocrit and the morphology of erythrocytes, was investigated here via fluorescence microscopy using FITC-concanavalin A binding to cells. The effect of melittin on the membrane capacitance and resistance of model lipid bilayers was probed via electrochemical impedance spectroscopy. Lipid bilayer capacitance was higher in the presence of 0.10 g/L melittin compared to that in the control, which is likely related to bilayer thinning and alterations of the dielectric permittivity of melittin-treated membranes. The biomolecule interactions with red blood cells were probed in physiological media in which the surface of erythrocyte membranes was negatively charged. Surface parameters of erythrocytes are reported upon IgG/hematin and IgG/melittin treatment. Pooled IgG in the presence of melittin, preincubated IgG/hematin preparations promoted a significant decrease in the electrokinetic potential of erythrocytes (Rh-positive). A malondialdehyde (MDA) assay revealed a high rate of lipid peroxidation in erythrocytes treated with IgG/hematin or IgG/melittin preparations. This finding might be a result of pooled IgG interactions with the hematin molecule and the subsequent conformational changes in the protein molecule altering the electrokinetic properties of the erythrocyte membrane related to the Rh group type of erythrocytes. The pooled IgG and hematin are reported to have important consequences for the biophysical understanding of the immunopathological mechanisms of inflammatory, autoimmune and antibody-mediated pathological processes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Erythrocyte Membrane Biophysical Changes Mediated by Pooled Immunoglobulin G and Hematin: Electrokinetic and Lipid Peroxidation Studies

et al. 2023

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“…Although most of the direct isolation of membrane protein studies used bacteria, the use of mammalian and other cell types and other organisms such as viruses, insects, and fungi will become common in the near future. It would be exciting to explore the use of the nanodiscs technology for other membrane-related studies as well [ 67 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 ].…”

Section: Conclusion and Future Scopementioning

confidence: 99%

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

Krishnarjuna

Ramamoorthy

2022

Biomolecules

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Atomic-resolution structural studies of membrane-associated proteins and peptides in a membrane environment are important to fully understand their biological function and the roles played by them in the pathology of many diseases. However, the complexity of the cell membrane has severely limited the application of commonly used biophysical and biochemical techniques. Recent advancements in NMR spectroscopy and cryoEM approaches and the development of novel membrane mimetics have overcome some of the major challenges in this area. For example, the development of a variety of lipid-nanodiscs has enabled stable reconstitution and structural and functional studies of membrane proteins. In particular, the ability of synthetic amphipathic polymers to isolate membrane proteins directly from the cell membrane, along with the associated membrane components such as lipids, without the use of a detergent, has opened new avenues to study the structure and function of membrane proteins using a variety of biophysical and biological approaches. This review article is focused on covering the various polymers and approaches developed and their applications for the functional reconstitution and structural investigation of membrane proteins. The unique advantages and limitations of the use of synthetic polymers are also discussed.

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“…The electrokinetic (zeta) potential, an electrochemical property of erythrocyte surfaces, is determined by the net electrical charge of surface-exposed molecules. The surfaces of erythrocyte membranes carry a net negative charge at a neutral pH, and sialic acid residues are responsible for most of the negative charges on the cell surface ( Mehrishi and Bauer, 2002 ; Karemore and Avari, 2019 ; Doltchinkova et al, 2021 ). The erythrocyte membrane is surrounded by a fixed layer of cations and coions in the medium (Galassi and Wilke, 2021) by a diffuse double layer (DDL) of a mixture of cations and anions.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic properties of healthy and β-thalassemia erythrocyte membranes under in vitro exposure to static magnetic field

Doltchinkova,

Lozanova,

Rukova

et al. 2023

Front. Chem.

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Introduction: The current understanding of the biological impacts of a static magnetic field (SMF) is restricted to the direct interactions of the magnetic field with biological membranes. The electrokinetic (zeta) potential is an electrochemical property of erythrocyte surfaces which was negatively charged in physiological media after SMF exposure (0.1‒2.0 T).Methods: The novel data about electrokinetic parameters of the erythrocytes is determined by microelectrophoresis after SMF-exposure in norm and heterozygous β-thalassemia. The methods of light scattering, lipid peroxidation, fluorescence microscopy are used.Results: The electrokinetic potential of erythrocytes in norm is increased after SMF intensities due to enhanced negatively exposed charges on the outer surface of the membrane accompanied by an increase in light scattering where changes in cell morphology are observed. Conversely, a decrease in the zeta potential of β-thalassemia erythrocytes upon SMF-treatment was determined because of the reduction in the surface electrical charge of the membranes, where a significant decrease in light scattering at 1.5 T and 2.0 T was recorded. Exposure to SMF (0.5–2.0 T) was associated with an increase in the malondialdehyde content in erythrocytes. Biophysical studies regarding the influence of SMF on the electrostatic free energy of cells shows an increase in negative values in healthy erythrocytes, which corresponds to the implementation of a spontaneous process. This is also the process in β-thalassemia cells after SMF exposure with lower negative values of free electrostatic energy than erythrocytes in norm.Discussion: The effect of static magnetic field (SMF 0.1–2.0 T) on the electrokinetic and morphological characteristics of erythrocytes in norm and β-thalassemia is determined and correlated with the increase/reduction in surface charge and shrinkage/swelling of the cells, respectively. Lipid peroxidation of healthy and β-thalassemia erythrocytes caused an enhancement of lipid peroxidation because of the higher concentrations of TBARS products in cellular suspension. SMF (0.1‒2.0 T) altered the spontaneous chemical processes with negative values of electrostatic free energy of erythrocytes in norm and β-thalassemia accompanied by a lower FITC-Concanavalin A binding affinity to membrane receptors (SMF 2.0 T). The electrokinetic properties of human erythrocytes in norm and β-thalassemia upon SMF treatment and their interrelationship with the structural-functional state of the membrane were reported. The presented work would have future fundamental applications in biomedicine.

show abstract

Viper toxins affect membrane characteristics of human erythrocytes

Cited by 6 publications

References 50 publications

Erythrocyte Membrane Biophysical Changes Mediated by Pooled Immunoglobulin G and Hematin: Electrokinetic and Lipid Peroxidation Studies

Erythrocyte Membrane Biophysical Changes Mediated by Pooled Immunoglobulin G and Hematin: Electrokinetic and Lipid Peroxidation Studies

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

Electrokinetic properties of healthy and β-thalassemia erythrocyte membranes under in vitro exposure to static magnetic field

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