Violet Mink Develop an Acute Disease after Experimental Infection with Aleutian Disease Virus (ADV) Isolate ADV SL3

Haas, Ludwig; Wohlsein, P.; Trautwein, G.; Stolze, Birgit; Kaaden, Oskar‐Rüger

doi:10.1111/j.1439-0450.1990.tb01033.x

Cited by 6 publications

(7 citation statements)

References 27 publications

(27 reference statements)

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“…The results of our study reveal a distinct extranuclear localization of viral antigen and fail to demonstrate the nuclear presence of A D viral proteins generally accepted as evidence of ADV replication in vitro (VAN DAWEN et al, 1983). This confirms findings obtained in previous immunohistological and in situ-hybridization studies performed on lymphoid tissues from experimentally infected mink indicating a viral sequestration in this particular location of lymphoid follicles (ALEXANDERSEN et al, 1988;HAAS et al, 1990). It is unlikely that follicular dendritic cells are major target cells, because their intrinsic mitotic rate, even in immunized animals is very low (TEW et al, 1982) and as known from other parvoviruses an active D N A metabolism is a requirement for parvoviral replication (COTMORE and TATTERSALL, 1987).…”

Section: Discussionsupporting

confidence: 88%

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Antigen Distribution in Organs of Mink with Aleutian Disease Parvovirus Infection

Wohlsein

Trautwein

Stolze

et al. 1990

Journal of Veterinary Medicine, Series B

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Summary On tissues from naturally infected non‐Aleutian mink an immunohistological study was performed using monoclonal antibodies and the immunoperoxidase method. Structural proteins of ADV were demonstrated in cryosections and in ethanol‐fixed and paraffin‐embedded material which provide antigen detection in a similar amount together with good histological structure. In lymphoid organs viral antigen was restricted to B‐cell areas, particularly lymphoid follicles. The pattern of antigen distribution was typical for follicular dendritic cells which are capable to retain immune complexes. Beside macrophages in the interior of lymphoid follicles most likely proliferating B‐lymphoblasts reveal nuclear and cytoplasmatic presence of structural proteins indicating viral replication. Cells of the mononuclear phagocyte system such as cells of lymphatic sinuses and hepatic Kupffer cells harbor viral protein in the cytoplasm, probably resulting from phagocytosis of immune complexes. Renal glomeruli were consistently negative for virus antigen whereas in interstitial infiltrates cells resembling macrophages stained positive for ADV structural proteins.

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Section: Discussionsupporting

confidence: 88%

“…According to previous immunohistological findings (PORTER et al, 1969;RACE et al, 1986;HAAS et al, 1990) viral antigen was found in the cytoplasm of Kupffer cells. A prerequisite for the demonstration of viral proteins was to perform an elution procedure on cryosections.…”

Section: Discussionmentioning

confidence: 66%

Antigen Distribution in Organs of Mink with Aleutian Disease Parvovirus Infection

Wohlsein

Trautwein

Stolze

et al. 1990

Journal of Veterinary Medicine, Series B

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“…The target cells of ADV in vivo are lymphoid cells, most likely of the B-cell lineage (KAADEN et al, 1986;HAAS et al, 1990) and cells of the macrophage lineage (KANNO et al, 1992). By strand-specific in situ hybridization viral replication could be demonstrated in lymph nodes, spleen, peripheral blood lymphocytes and bone marrow cells of infected mink (ALEXANDERSEN et al, 1988;HAAS et al, 1990 Thus a block during viral DNA replication or transcription in (certain) lymphoid target cells (bone marrow) might lead to an inefficient nicking of the Mccl RF DNA. This could result in a restricted infection with low-level virion synthesis, and the resulting permanent antigenic stimulus may be one mechanism contributing to the Aleutian disease virus persistence in wiwo.…”

Section: The MCCL Rf D N a Is An Intermediate Of Aleutian Disease Parmentioning

confidence: 99%

The Monomer Covalently Closed Linear Replicative Form DNA of Aleutian Disease Parvovirus is Infectious After Transfection into Permissive Cells

Truyen

Schelp

Löchelt

et al. 1993

Journal of Veterinary Medicine, Series B

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Summary The recently described monomer covalently closed linear replicative form DNA (Mccl RF DNA) of Aleutian disease parvovirus (ADV) is an infectious intermediate of the viral DNA replication cycle. Transfection of highly purified Mccl RF DNA into susceptible feline kidney cells (CCC clone 81 cells) resulted in viral DNA replication, expression of viral proteins and synthesis of infectious progeny virus. Mccl RF DNAs generated under permissive (32 °C) or non‐permissive (37 °C) conditions were shown to be biologically indistinguishable. The accumulation of the Mccl RF DNA form at the non‐permissive temperature in vitro strongly resembles that in bone marrow cells of naturally infected mink and may reflect one mechanism contributing to virus persistence of ADV in vivo. Zusammenfassung Die monomer kovalent‐geschlossene lineare replikative Form (Mccl RF)‐DNA des Aleutian Disease Parvovirus (ADV) ist ein infektiöses Intermediat im Rahmen der viralen DNA‐Replikation. Transfektion hochgereinigter Mccl RF DNA in feline Nierenzellen CCC clone 81 induzierte virale DNA‐Replikation, die Expression viraler Proteine und die Synthese infektiöser Virionen. Mccl RF DNAs aus infizierten Zellen inkubiert unter permissiven (32 °C) oder nicht‐permissiven (37 °C) Bedingungen waren funktionell nicht unterscheidbar. Die relative Anhäufung dieser RF‐DNA‐Form unter der nicht‐permissiven Temperatur in vitro ähnelt stark der in Knochenmarkszellen infizierter Nerze in vivo, und ist möglicherweise eine Ursache der Viruspersistenz im Rahmen der Aleutenkrankheit der Nerze.

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“…However, the specific virus strain as well as the age and genotype of the mink strongly influence whether this classical picture occurs after infection (23,40,42,43).…”

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The relationship between capsid protein (VP2) sequence and pathogenicity of Aleutian mink disease parvovirus (ADV): a possible role for raccoons in the transmission of ADV infections

Oie

Durrant

Wolfinbarger

et al. 1996

J Virol

110

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Aleutian mink disease parvovirus (ADV) DNA was identified by PCR in samples from mink and raccoons on commercial ranches during an outbreak of Aleutian disease (AD). Comparison of DNA sequences of the hypervariable portion of VP2, the major capsid protein of ADV, indicated that both mink and raccoons were infected by a new isolate of ADV, designated ADV-TR. Because the capsid proteins of other parvoviruses play a prominent role in the determination of viral pathogenicity and host range, we decided to examine the relationship between the capsid protein sequences and pathogenicity of ADV. Comparison of the ADV-TR hypervariable region sequence with sequences of other isolates of ADV revealed that ADV-TR was 94 to 100% related to the nonpathogenic type 1 ADV-G at both the DNA and amino acid levels but less than 90% related to other pathogenic ADVs like the type 2 ADV-Utah, the type 3 ADV-ZK8, or ADV-Pullman. This finding indicated that a virus with a type 1 hypervariable region could be pathogenic. To perform a more comprehensive analysis, the complete VP2 sequence of ADV-TR was obtained and compared with that of the 647amino-acid VP2 of ADV-G and the corresponding VP2 sequences of the pathogenic ADV-Utah, ADV-Pullman, and ADV-ZK8. Although the hypervariable region amino acid sequence of ADV-TR was identical to that of ADV-G, there were 12 amino acid differences between ADV-G and ADV-TR. Each of these differences was at a position where other pathogenic isolates also differed from ADV-G. Thus, although ADV-TR had the hypervariable sequence of the nonpathogenic type 1 ADV-G, the remainder of the VP2 sequence resembled sequences of other pathogenic ADVs. Under experimental conditions, ADV-TR and ADV-Utah were highly pathogenic and induced typical AD in trios of both Aleutian and non-Aleutian mink, whereas ADV-Pullman was pathogenic only for Aleutian mink and ADV-G was noninfectious. Trios of raccoons experimentally inoculated with ADV-TR and ADV-Utah all became infected with ADV, but only a single ADV-Pullman-inoculated raccoon showed evidence of infection. Furthermore, none of the ADV isolates induced pathological findings of AD in raccoons. Finally, when a preparation of ADV-TR prepared from infected raccoon lymph nodes was inoculated into mink and raccoons, typical AD was induced in Aleutian and non-Aleutian mink, but raccoons failed to show serological or pathological evidence of infection. These results indicated that raccoons can become infected with ADV and may have a role in the transmission of virus to mink but that raccoon-to-raccoon transmission of ADV is unlikely.

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Violet Mink Develop an Acute Disease after Experimental Infection with Aleutian Disease Virus (ADV) Isolate ADV SL3

Cited by 6 publications

References 27 publications

Antigen Distribution in Organs of Mink with Aleutian Disease Parvovirus Infection

Antigen Distribution in Organs of Mink with Aleutian Disease Parvovirus Infection

The Monomer Covalently Closed Linear Replicative Form DNA of Aleutian Disease Parvovirus is Infectious After Transfection into Permissive Cells

The relationship between capsid protein (VP2) sequence and pathogenicity of Aleutian mink disease parvovirus (ADV): a possible role for raccoons in the transmission of ADV infections

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