Vinpocetine and α‐tocopherol prevent the increase in DA and oxidative stress induced by 3‐NPA in striatum isolated nerve endings

Abstract

Vinpocetine is a neuroprotective drug that exerts beneficial effects on neurological symptoms and cerebrovascular disease. 3-nitropropionic acid (3-NPA) is a toxin that irreversibly inhibits succinate dehydrogenase, the mitochondrial enzyme that acts in the electron transport chain at complex II. In previous studies in striatum-isolated nerve endings (synaptosomes), we found that vinpocetine decreased dopamine (DA) at expense of its main metabolite 3,4-dihydroxyphenylacetic acid (DOPAC), and that 3-NPA increased DA, reactive oxygen species (ROS), DA-quinone products formation, and decreased DOPAC. Therefore, in this study, the possible effect of vinpocetine on 3-NPA-induced increase in DA, ROS, lipid peroxidation, and DA-quinone products formation in striatum synaptosomes were investigated, and compared with the effects of the antioxidant a-tocopherol. Results show that the increase in DA induced by 3-NPA was inhibited by both 25 lM vinpocetine and 50 lM a-tocopherol. Vinpocetine, as a-tocopherol, also inhibited 3-NPA-induced increase in ROS (as judged by DCF fluorescence), lipid peroxidation (as judged by TBA-RS formation), and DA-quinone products formation (as judged by the nitroblue tetrazolium reduction method). As in addition to the inhibition of complex II exerted by 3-NPA, 3-NPA increases DA-oxidation products that in turn can inhibit other sites of the respiratory chain, the drop in DA produced by vinpocetine and a-tocopherol may importantly contribute to their protective action from oxidative damage, particularly in DA-rich structures.

Fish Oil Prophylaxis Protects against 3-Nitropropinic Acid-Induced Oxidative Impairments in Striatum and Cerebellum in Male Rat Brain

“…NPA administration caused robust oxidative stress as evidenced by elevated oxidative markers in both cytosol and mitochondria of brain regions. These results are in agreement with previous findings among NPA models of neurotoxicity [9,38]. Evidence in experimental animals has clearly shown that NPA affects different brain regions, and striatal neurons were more vulnerable [39].…”

Evaluation of Therapeutic Efficacy of Vinpocetine in Adjuvant Induced Arthritis Model in Rats

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Resveratrol protects against homocysteine‐induced cell damage via cell stress response in neuroblastoma cells

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