Vinegar amount in the process affected the components of vinegar-baked Radix Bupleuri and its hepatoprotective effect

Abstract

BackgroundBupleuri Radix (in Chinese Chaihu), the dried roots of Bupleurum Chinense DC, is a traditional Chinese medicine widely used to treat fever, hepatitis, jaundice, nephritis, dizziness. When baked with vinegar, its effect is more focused on liver related disease. This paper was undertaken to determine the best vinegar amount in the processing and explore its key efficacy components.MethodsHepatoprotective effects of Radix Bupleuri after processing with different amount of vinegar (1:5, 2:5, 3:5) were investigated on liver hurt rats, and the change of constituents were analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).ResultsWith the increasing amount of vinegar, the hepatoprotective effects of vinegar-baked Radix Bupleuri (VBRB) and the content of saikosaponin b2 increased.ConclusionThese results suggested that vinegar amount in the process affected the pharmacological effect of VBRB significantly and saikosaponin b2 may be the key efficacy component of it.

A rapid classification and identification method applied to the analysis of glycosides in Bupleuri radix and liquorice by ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

“…Triterpene saponins are common and the main effective components in BR and liquorice, and triterpene saponins have received increasing attention in recent years because of their variety and extensive biological activity. Pharmacological studies have revealed that saponins from BR have anti‐inflammatory, immunoregulation, and anti‐hepatic fibrosis effects . In addition, a wide array of biological activities has been ascribed to saponins from liquorice, including anti‐inflammatory, antiarrhythmia and hepatoprotective effects, among others .…”

Multielements determination and metal transfer investigation in herb medicine Bupleuri Radix by inductively coupled plasma‐mass spectrometry

“…Zhao, Wang, et al. (2016); Zhao, We, et al. (2016) demonstrated that the transfer ratios (TRs) of the investigated elements were different and that it might depend on the types of elements, the plant species, and the extraction methods.…”

“…In this study, we found that Co and As had the highest TRs values, thus the two elements should be closely monitored to prevent further transferring from herbal medicines to decoctions. In terms of the TRs research on heavy metals, water extraction can significantly reduce the contents (Zhao, Wang, et al., 2016; Zhao, We, et al., 2016). The result also points out that the PaoZhi method may help heavy metals in the herb dissolve more easily into its decoction.…”

“…In other words, only the elements that are transferred to decoction liquids maybe absorbed. Several researchers have proven its validity and usefulness (Da, Hauser‐Davis, Suzuki, & Vitória, 2017; Hu et al., 2017; Liao et al., 2014; Zhao, Wang, Zhao, & Xiang, 2016; Zhao, Wei, Shu, Kong, & Yang, 2016), because of this, the metal transfer investigation method was applied in this study to evaluate the safety control of TCMs.…”

“…), has several useful pharmacological effects. These include alleviating ailments such as influenza, dizziness, lung diseases, chronic gastritis, and cancer (ChP., 2015; Qin, Liu, & Yuan, 2013; Zhao, Wang, et al., 2016; Zhao, We, et al., 2016). In addition, several phytochemical studies have shown that active compounds such as saikosaponin, glycosides, flavonoids, and volatile oil can be isolated from Bupleurum L. (Zhao, Li, Yue, Zhang, & Dou, 2012).…”