Vine Tea (Ampelopsis grossedentata) Extract Attenuates CCl₄‐Induced Liver Injury by Restoring Gut Microbiota Dysbiosis in Mice

Abstract

Scope Vine tea (Ampelopsis grossedentata), a traditional Chinese tea, has displayed various biological activities. The authors aim to investigate the effect of Vine Tea (Ampelopsis grossedentata) extract (VTE) on carbon tetrachlorid (CCl4)induced acute liver injury (ALI) in mice and to explore the underlying role of gut microbiota during the treatment. Methods and Results C57BL/6J mice injected with CCl4 are treated with VTE for 6 weeks. By using H&E staining, immunofluorescence staining, quantitative real‐time (qRT)‐PCR, and western blot, it is shown that VTE treatment significantly ameliorates hepatocyte necrosis, alleviates the mRNA levels of toll‐like receptor 4 (Tlr4), interleukin (Il)‐6, inducible nitric oxide synthase (iNOS), acetyl‐CoA carboxylase 1 (Acc1), and increases the mRNA levels of peroxisome proliferator‐activated receptor gamma (Ppar‐γ) and 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase (Hmg‐coar) compared to the CCl4 group. Also, VTE abrogates the decreased mRNA expressions of zonula occludens‐1 (Zo‐1), Occludin, and Mucin1 in colon tissues. Using microbial 16S rDNA sequencing, VTE treatment significantly downregulates the abundances of some harmful intestinal bacteria like Helicobacter and Oscillibacter. In contrast, VTE upregulates the contents of several beneficial bacteria, such as Ruminococcaceae_UCG‐014 and Eubacterium_fissicatena_group. Further, VTE fails to improve ALI in the mice with gut microbiota depletion using antibiotic treatment. Conclusions The studies suggest that VTE exhibits a protective effect against CCl4‐induced ALI in mice by alleviating hepatic inflammation, suppressing intestinal epithelial barrier injury, and restoring gut microbiota dysbiosis.

Lactobacillus plantarum ZJUIDS14 alleviates non-alcoholic fatty liver disease in mice in association with modulation in the gut microbiota

“…Coprostanoligenes is a central fecal microbial group for individuals on HFD, mainly through sphingosine to mediate blood lipid metabolism ( 60 ). Ruminococcaceae UCG-014 , Ruminiclostridium 1 , and Allobaculum are SCFA-producing microbiota and play important roles in maintaining a healthy gastrointestinal tract ( 61 – 63 ), improving NASH ( 64 ), and alleviating adipose tissue inflammation ( 65 ). Consistent with other studies ( 66 , 67 ), HFD-fed mice had fewer Ruminococcaceae UCG-014, Ruminiclostridium 1 , and Allobaculum than the NFD group, while L. plantarum ZJUIDS14 treatment reversed their relative abundance in the colon of HFD-fed mice.…”

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

“…Besides, the relative abundance of Candi-datus_Arthromitus, Chryseobacterium, and [Eubacterium]_ fissicatena_group was lower in the DF group than that in the FM group. These bacteria were frequently considered as a pathogenic bacterium [79][80][81][82][83]. However, the relative abundance of potential probiotics Lactobacillus, Dubosiella,…”

Targeted modulation of gut microbiota by traditional Chinese medicine and natural products for liver disease therapy

“…In addition, the extract of ginseng and the seeds of Zizyphus jujuba var. spinosa (GS) significantly increased the relative abundance of Lactobacillus and Bifidobacterium and decreased Streptococcus, Escherichia coli-Shigella, Veillonella and Enterococcus in rats, suggesting that GS extract may be a promising AILD therapeutic drug by balancing the structure and diversity of gut microbiota (46).…”

“…Specifically, VTE significantly reduced the content of harmful gut microbiota, such as Helicobacter and Oscillibacter , and increased the abundance of beneficial gut microbiota, such as Ruminococcaceae_UCG-014 and Eubacterium_fissicatena _group. The gradually restored gut microbiota not only reduces liver inflammation and oxidative stress but also enhances the intestinal barrier by promoting the expression of zonula ZO-1, Occludin-1, and Mucin-1 in intestinal tissues and ultimately achieves the effect of reducing hepatotoxicity and ALI ( 47 ). When the gut microbiota was depleted, the disappearance of VTE efficacy verified its targeted regulatory mechanism on the gut microbiota.…”