Vindoline Inhibits RANKL-Induced Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss in Mice

Zhan, Yunfei; Liang, Jiamin; Tian, Kun; Che, Zhigang; Wang, Ziyi; Yang, Xue; Su, Yuangang; Lin, Xiaofeng; Song, Fangming; Zhao, Jinmin; Xu, Jiake; Liu, Qian; Zhou, Bo

doi:10.3389/fphar.2019.01587

Cited by 21 publications

(22 citation statements)

References 38 publications

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“…Bone quality maintenance is closely related with bone metabolism activity, mainly containing bone resorption and remodeling, which were driven by osteoclast and osteoblast activities ( Zhan et al., 2020 ). Therefore, osteoclast and osteoblast activities related enzymes and factors in serum were further investigated in this current research.…”

Section: Discussionmentioning

confidence: 99%

Effect of photoperiod on eggshell quality and quality characteristics of tibia, femur, and ulna in laying ducks

et al. 2021

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We investigated the effect of photoperiod on eggshell quality, bone quality characteristics and bone metabolism related enzymes and factors in laying ducks. After adaption, 300 Jinding laying ducks (252-day-old) were randomly divided into 5 treatments, receiving 12 L (hours of light):12 D (hours of darkness), 14L:10D, 16L:8D, 18L:6D or 20L:4D, respectively. Each group had 6 replicates of 10 birds each. The feeding experiment lasted 8 wk. Compared with 12L:12D, the higher values of eggshell breaking strength occurred in ≥18 h photoperiods at the end of 6 wk, and in ≥16 h photoperiods at the end of 4 wk, with the common highest values in 18 h photoperiod ( P ˂ 0.05). Besides, 18L:6D had higher values of ultimate load Fu and cortical cross-sectional area A in tibia, femur, and ulna ( P ˂ 0.05), compared with 12L:12D. The higher values of proximal bone mineral content ( BMC ; tibia), distal BMC (ulna), total Ca (tibia), and cortical volumetric bone mineral density ( vBMC ; tibia and ulna) were observed in 16L:8D and 18L:6D treatments ( P ˂ 0.05). Meanwhile, 18 h photoperiod group had the higher proximal BMC (femur) and total Ca in ulna ( P ˂ 0.05). In serum, compared with 12L:12D group, the higher ALP activity occurred in ≥16 h photoperiods (0:00 and 18:00), with the highest values in 18L:6D treatment ( P ˂ 0.05); the higher values of TGF-β (6:00) and OC (6:00 and 18:00) were simultaneously observed in 18 h photoperiod ( P ˂ 0.05). Moreover, values of trACP activity, TNF-α and IL-6 contents decreased in ≥18 h photoperiods at 0:00 ( P ˂ 0.05), compared with 12L:12D group. To sum up, an appropriate photoperiod could improve eggshell quality, bone strength and mineral content through increasing osteogenesis during the light time and decreasing resorption activity during the dark, and 18 h is an adequate photoperiod for the eggshell and bone quality of laying ducks.

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Section: Discussionmentioning

confidence: 99%

Effect of photoperiod on eggshell quality and quality characteristics of tibia, femur, and ulna in laying ducks

et al. 2021

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“…Osteoclasts and osteoblasts are key players in retaining bone mass and are the major cells involved in bone remodelling (Zhan et al, 2019). Several miRNAs that participate in the differentiation of osteoblasts have been identified.…”

Section: Discussionmentioning

confidence: 99%

miR-452-3p inhibited osteoblast differentiation by targeting Smad4

Wang

Kong

et al. 2021

PeerJ

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Osteoblast differentiation is a complex process that is essential for normal bone formation. A growing number of studies have shown that microRNAs (miRNAs) are key regulators in a variety of physiological and pathological processes, including osteogenesis. In this study, BMP2 was used to induce MC3T3-E1 cells to construct osteoblast differentiation cell model. Then, we investigated the effect of miR-452-3p on osteoblast differentiation and the related molecular mechanism by RT-PCR analysis, Western blot analysis, ALP activity, and Alizarin Red Staining. We found that miR-452-3p was significantly downregulated in osteoblast differentiation. Overexpression miR-452-3p (miR-452-3p mimic) significantly inhibited the expression of osteoblast marker genes RUNX2, osteopontin (OPN), and collagen type 1 a1 chain (Col1A1), and decreased the number of calcium nodules and ALP activity. In contrast, knockdown miR-452-3p (miR-452-3p inhibitor) produced the opposite effect. In terms of mechanism, we found that Smad4 may be the target of miR-452-3p, and knockdown Smad4 (si-Smad4) partially inhibited the osteoblast differentiation enhanced by miR-452-3p. Our results suggested that miR-452-3p plays an important role in osteoblast differentiation by targeting Smad4. Therefore, miR-452-3p is expected to be used in the treatment of bone formation and regeneration.

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“…During osteoclast differentiation, F-actin rings are considered as the characteristic structure of mature osteoclasts and is indispensable for its bone resorption activity. 23 In this study, we used Actin-Tracker to identify the formation and morphology of F-actin. Consistent with inhibition of osteoclast formation, the formation of podosomal actin belts was disrupted and the reduced actin ring formation was associated with intervention concentration of OB (Figure 3A).…”

Section: Ob Exhibits Anti-resorption Effect In Vitromentioning

confidence: 99%

Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity

Huang¹,

Wang²,

Lu³

et al. 2021

DDDT

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Background: Osteoclasts are the major players in bone resorption and have always been studied in the prevention and treatment of osteoporosis. Previous studies have confirmed that a variety of flavonoids inhibit osteoporosis and improve bone health mainly through inhibiting osteoclastogenesis. Oroxin B (OB) is a flavonoid compound extracted from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent, exerts potent antitumor and antiinflammation effect, but its effect on osteoclastogensis remains unknown. Methods: We comprehensively evaluated the effect of OB on the formation and function of osteoclasts and the underling mechanism by bone marrow-derived macrophage in vitro. In vivo, we used mice ovariectomized model to verify the protective effect of OB. Results: OB was found to inhibit osteoclast formation and bone resorption function in vitro, in a dose-dependent manner and the increased osteoclastic-related genes induced by RANKL (NFATc1, c-fos, cathepsin K, RANK, MMP9 and TRAP) were also attenuated following OB treatment. Mechanistical investigation showed OB abrogated the increased phosphorylation level of MAPK and NF-κB pathway, and diminished the expression of the vital transcription factors for osteoclastogenesis. OB also prevented ovariectomy (OVX)-induced bone loss by inhibiting osteoclast formation and activity in mice. Conclusion:Our study demonstrated that OB may act as an anti-osteoporosis agent by inhibiting osteoclast maturation and attenuating bone resorption.

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Vindoline Inhibits RANKL-Induced Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss in Mice

Cited by 21 publications

References 38 publications

Effect of photoperiod on eggshell quality and quality characteristics of tibia, femur, and ulna in laying ducks

Effect of photoperiod on eggshell quality and quality characteristics of tibia, femur, and ulna in laying ducks

miR-452-3p inhibited osteoblast differentiation by targeting Smad4

Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity

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