Vinculin controls focal adhesion formation by direct interactions with talin and actin

Humphries, Jonathan D.; Wang, Pengbo; Streuli, Charles; Geiger, B.; Ballestrem, Christoph

doi:10.1083/jcb.200703036

Cited by 782 publications

(847 citation statements)

References 55 publications

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“…1A). Consistent with previous studies [1][2][3][4], vinculin knockdown cells showed lower cell-cell and cell-ECM adhesions compared with mock-transfected HSC-4 cells (Fig. 1B).…”

Section: Vinculin Knockdown Promotes Mt1-mmp-mediated Prommp-2 Activasupporting

confidence: 92%

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Vinculin negatively regulates transcription of MT1-MMP through MEK/ERK pathway

Yoshimoto

Takino

et al. 2014

Biochemical and Biophysical Research Communications

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“…1A). Consistent with previous studies [1][2][3][4], vinculin knockdown cells showed lower cell-cell and cell-ECM adhesions compared with mock-transfected HSC-4 cells (Fig. 1B).…”

Section: Vinculin Knockdown Promotes Mt1-mmp-mediated Prommp-2 Activasupporting

confidence: 92%

“…Viculin has been implicated as an inhibitor of cell migration, as it enhanced cellular adhesiveness [1][2][3][4]. Loss or reduced expression of vinculin is related to the metastatic potential of squamous cell carcinomas (SCC), although in situ SCC exhibits high level expression of vinculin [5].…”

Section: Introductionmentioning

confidence: 99%

Vinculin negatively regulates transcription of MT1-MMP through MEK/ERK pathway

Yoshimoto

Takino

et al. 2014

Biochemical and Biophysical Research Communications

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“…4 A-B) presented a clear pattern of adhesion complexes, identified as green spots (vinculin aggregates) that are mainly localized at the periphery of the cell, in connection with axonal growth. Such a protein is indeed associated in characteristic plaques during formation of focal adhesion complex (31)(32)(33). Cells seeded onto rough substrates (Fig.…”

Section: Resultsmentioning

confidence: 97%

Neurons sense nanoscale roughness with nanometer sensitivity

Brunetti

Maiorano

Rizzello

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

227

207

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The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for the design of smart nanobiomaterials with active biological functionalities. In this frame we investigated the response of human neuroblastoma cell line (SH-SY5Y) to gold surfaces with different levels of nanoroughness. To achieve a precise control of the nanoroughness with nanometer resolution, we exploited a wet chemistry approach based on spontaneous galvanic displacement reaction. We demonstrated that neurons sense and actively respond to the surface nanotopography, with a surprising sensitivity to variations of few nanometers. We showed that focal adhesion complexes, which allow cellular sensing, are strongly affected by nanostructured surfaces, leading to a marked decrease in cell adhesion. Moreover, cells adherent on nanorough surfaces exhibit loss of neuron polarity, Golgi apparatus fragmentation, nuclear condensation, and actin cytoskeleton that is not functionally organized. Apoptosis/necrosis assays established that nanoscale features induce cell death by necrosis, with a trend directly related to roughness values. Finally, by seeding SH-SY5Y cells onto micropatterned flat and nanorough gold surfaces, we demonstrated the possibility to realize substrates with cytophilic or cytophobic behavior, simply by fine-tuning their surface topography at nanometer scale. Specific and functional adhesion of cells occurred only onto flat gold stripes, with a clear self-alignment of neurons, delivering a simple and elegant approach for the design and development of biomaterials with precise nanostructuretriggered biological responses.nanobiointeractions | nanostructures | patterning T he potential of nanomaterials to trigger specific cellular responses, such as interference and/or activation of defined pathways (1-3), is promising for the development of many important scientific fields, such as regenerative medicine, biotechnology, drug delivery, and nanotoxicity assessment. Initially, cellsmaterials interactions were tackled only from a chemical point of view, because environmental sensing by cells involves specific binding between cellular receptors and ECM ligands. However, recently there has been increasing evidence that the biological response is also affected by the physical properties of the material (4). In particular, it has been demonstrated that cells are influenced by the substrate topography (5, 6), rigidity (7, 8), anisotropy (9, 10), surface charge (11, 12), and wettability (13,14). From this perspective, cellular response to external stimuli goes far beyond the bare ability of the cell to chemically sense specific ECM ligands and includes a wide range of physical cues that are generated at, or act on, the interface between cells and the surrounding environment. For instance, it has been observed that micrometer-scale roughness may affect cell proliferation and morphology (15,16), because it provides a quasi-biomimetic microenvironment to the cells. How...

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“…In HeLa cells, Wrch1 was recruited to Src-induced podosomes only when they were also associated with vinculin (Ory et al, 2007). Vinculin is an essential regulator of focal adhesions (Humphries et al, 2007): it increases focal adhesion number and favors their maturation. On the contrary, we reported earlier that Wrch1 reduced focal adhesion number and inhibited their maturation (Ory et al, 2007).…”

Section: Discussionmentioning

confidence: 99%