Vinblastine inhibits platelet aggregation by a microtubule-independent mechanism, probably by its perturbing action on the plasma membrane

Hashizume, Takumi; Akiba, Suminori; Sato, Takashi; Fujii, Tatsuzo; Watanabe, Sadahiro; Sasaki, Junzo

doi:10.1016/0049-3848(88)90186-7

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Studies on the roleo fm icrotubules on platelet aggregation reported conflicting results. Vinblastine, an alkaloid that depolymerises microtubules, has beenreported to reduce ADP-induced platelet aggregation (10), althought his effect has later beena ttributedt op hysicalp erturbation of membrane lipid bilayer, independent of its activity to disassemble plateletmicrotubule system (11). The antimycotic nocodazole,amicrotubular inhibitor,has been shown to inhibit plateletaggregation by collagen, thrombin,adrenaline, polylysine, and lectins (12),aneffect thatmight also be explained by its inhibitoryeffect on tyrosine kinase activity (13).…”

Section: Introductionmentioning

confidence: 99%

Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets

Bouaziz

Amor²,

Woodard³

et al. 2007

Thromb Haemost

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We have investigated the intracellular mechanisms involved in microtubular remodelling by thrombin and its possible involvement in platelet aggregation and secretion. Platelet stimulation with thrombin induces a time- and concentration-dependent regulation of the microtubular content, which was found to be maximally effective at the concentration 0.1 U/ml. Thrombin (0.1 U/ml) evoked an initial decrease in the microtubule content detectable at 5 seconds (sec) and reached a minimum 10 sec after stimulation. The microtubular content then increased, exceeding basal levels again approximately 30 sec after stimulation. Inhibition of tyrosine phosphatases using vanadate abolished thrombin-induced microtubular depolymerisation while inhibition of tyrosine kinases by methyl-2,5-dihydroxycinnamate prevented microtubule polymerisation. Thrombin activates the cytosolic Bruton's tyrosine kinase (Btk) and Src proteins. Inhibition of Btk or Src by LFM-A13 or PP1, respectively, abolished thrombin-induced microtubular polymerisation, while maintaining intact its ability to induce initial depolymerisation. Microtubular disruption by colchicine significantly reduced thrombin-induced platelet aggregation and ATP secretion. Similar results were observed after inhibition of microtubular disassembly by paclitaxel. These findings indicate that thrombin induces microtubular remodelling by modifying the balance between protein tyrosine phosphorylation and dephosphorylation. The former seems to be required for microtubular polymerisation, while tyrosine dephosphorylation is required for microtubular depolymerisation. Both, initial microtubular disassembly and subsequent polymerisation are required for thrombin-induced platelet aggregation and secretion in human platelets.

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Section: Introductionmentioning

confidence: 99%

Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets

Bouaziz

Amor²,

Woodard³

et al. 2007

Thromb Haemost

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“…Hashizume et al (1988) suggested a secondary effect of vinblastine on the plasma membrane of platelets. They proposed that vinblastine interacts, non-specifically, with the phospholipids of the membrane to give rise to a physical perturbation of the bilayer.…”

Section: Discussionmentioning

confidence: 97%

Effects of microtubule agents on the spatial and electrical properties of the plasma membrane inChara corallina

Fisahn¹,

Lucas²

1990

Planta

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The freshwater algaChara corallina Klein ex Willd., em. R.D.W. (=C. australis R.Br.) develops alternating outward (acid) and inward (alkaline) current areas on its surface when illuminated. Exposure of cells to vinblastine, colchicine, or oryzalin caused a reduction in and a shifting of this extracellular current pattern. Removal of these agents from the bathing media resulted in regeneration of the initial current profile. Because these agents all affect tubulin, microtubules may be responsible for orchestrating the transmembrane currents responsible for the acid and alkaline banding phenomenon. Analysis of the membrane potential showed a fast depolarization after vinblastine exposure; however, analysis of the current-voltage curve did not show a change in membrane conductance. A 30-min colchicine treatment decreased the conductance of the plasma membrane with either an hyperor a depolarization in the membrane potential. In contrast, although a 9-h exposure to oryzalin caused a major reduction in the extra-cellular current pattern, only minor changes were observed in the membrane potential and conductance. However, in the presence of oryzalin, the time constants in the light response of the membrane potential increased over this 9-h period. Collectively, these results implicate an involvement of microtubules in spatial control of plasma-membrane transport events inC. corallina.

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Effects of vinblastine on cell membrane fluidity and the growth of SA-1 tumor in mice

et al. 1994

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Vinblastine inhibits platelet aggregation by a microtubule-independent mechanism, probably by its perturbing action on the plasma membrane

Cited by 6 publications

References 18 publications

Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets

Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets

Effects of microtubule agents on the spatial and electrical properties of the plasma membrane inChara corallina

Effects of vinblastine on cell membrane fluidity and the growth of SA-1 tumor in mice

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