Vinblastine-induced paracrystals and unusually large microtubules (macrotubules) in rat renal cells

Tyson, Greta E.; Bulger, Ruth Ellen

doi:10.1007/bf00307116

Cited by 51 publications

(8 citation statements)

References 45 publications

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“…First, the microtubule fragments had abnormally large diameters, about 44 nm. Similar large tubular structures, which were 3 1-57 nm in diameter, have been reported in cells under normal conditions [23], low temperature [22, 241, or chemical treatment [25], and sometimes called macrotubules. The mechanism whereby these different factors induced these macrotubules has not been fully elucidated [3,14,24,251.…”

Section: Discussionsupporting

confidence: 65%

Effect and Localization of Trifluralin in Plasmodium falciparum Gametocytes: An Electron Microscopic Study1

Kaidoh¹,

Nath²,

Fujioka³

et al. 1995

J Eukaryotic Microbiology

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Trifluralin, a herbicide which is known to bind to plant and algal tubulin, induced ultrastructural changes in the microtubules of the mature Plasmodium falciparum gametocytes in vitro. Trifluralin treatment led to disassembly of the well ordered subpellicular microtubules, whereas it had no effect on microtubules of human platelets or of rat neuronal cells in vitro. The disassembled microtubules showed fragmented large tubular structures, which frequently were associated with the pellicular membranes. Electron microscopic autoradiography showed radioactive trifluralin associated with the microtubule fragments. These results provide evidence that trifluralin selectively binds to microtubules in malaria parasites and causes disruption of their structure.

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Section: Discussionsupporting

confidence: 65%

Effect and Localization of Trifluralin in Plasmodium falciparum Gametocytes: An Electron Microscopic Study1

Kaidoh¹,

Nath²,

Fujioka³

et al. 1995

J Eukaryotic Microbiology

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“…When Heliozoa are subjected to low temperature, the microtubules increase in diameter from 220 to 340 A (Tilney and Porter, 1967). Tyson and Bulger (1973) found that larger microtubules are induced by vinblastine, and suggest that C-shaped tubules are an intermediate form. Applying tannic acid, Tilney et al (1973) have concluded that 13 subunits and their arrangement as protofilaments are universally constant with respect to both phylogeny and location, whether in a cilium or in the cytoplasm.…”

Section: Discussionmentioning

confidence: 97%

Microtubules with 15 subunits in cockroach epidermal cells

Nagano¹,

Suzuki²

1975

The Journal of Cell Biology

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Since Ledbetter and Porter (1964) described the 13 subunits which are visible in cross sections of negatively stained plant microtubules, subsequent observations have generally confirmed this number. By using Mizuhira's fixative composed of tannic acid and glutaraldehyde, it is easyto demonstrate the subunits of microtublules without optical reinforcement Cytoplasmic microtubules and sperm axonemes, fixed with Mizuhira's fixtive, similarly show 13 subunits (Mizuhira's and Futaesaku, 1971, 1972; Futaesaky et al., 1972; Tilney et al., 1973). This paper will describe a particular type of microtubule in insect epidermal cells fixed with the above fixative. The number of the subunits is found to be 15 in tranverse sections.

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“…However, as in all other studies of microtubule-macrotuhule interconversions, no structural intermediates were observed in Ochromonas. On the basis of the experimental evidence (Balasubramanian and Wetlaufer, 1966) that halothane can induce conformational changes in globular proteins, Hinkley and Samson (1972) suggested that macrotubules in anesthetictreated axons are reassembled from halothanemodified microtubular subunit strands. Also, Tyson and Bulger (1973) have hypothesized that the formation of macrotubules in cells exposed to vinblastine may result from a vinblastineinduced conformation change or the stabilization of a particular conformation of microtubule protein . It is tempting to speculate that IPC (and CIPC) may have a similar direct molecular action on microtubule protein in Ochromonas .…”

Section: Discussionmentioning

confidence: 99%

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Brown

Bouck

1974

The Journal of Cell Biology

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The role of microtubules and microtubule nucleating sites in the unicell, Ochromonas has been examined through the use of two mitotic inhibitors, isopropyl N-phenylcarbamate (IPC) and isopropyl N-3-chlorophenyl carbamate (CIPC) . Although IPC and CIPC have little or no effect on intact microtubules, the assembly of three separate sets of microtubules in Ochromonas has been found to be differentially affected by IPC and CIPC . The assembly of flagellar microtubules after mechanical deflagellation is partially inhibited ; the reassembly of rhizoplast microtubules after pressure depolymerization is totally inhibited (however, macrotubules may form at the sites of microtubule initiation or elsewhere) ; and, the reassembly of the beak set of microtubules after pressure depolymerization may be unaffected although similar concentrations of IPC and CICP completely inhibit microtubule regeneration on the rhizoplast . These effects on microtubule assembly, either inhibitory or macrotubule inducing, are fully reversible . The kinetics of inhibition and reversal are found to be generally similar for both flagellar and cell shape regeneration . Incorporation data suggest that neither IPC nor CIPC has significant effects on protein synthesis in short term experiments . Conversely, inhibiting protein synthesis with cycloheximide has little effect on microtubule regeneration when IPC or CIPC is removed . Although the exact target for IPC and CIPC action remains uncertain, the available evidence suggests that the microtubule protein pool or the microtubule nucleating sites are specifically and reversibly affected . Comparative experiments using the mitotic inhibitor colchicine indicate some similarities and differences in its mode of action with respect to that of IPC and CIPC on assembly and disassembly of microtubules in these cells .In the first two papers of this series (Bouck and ently independent sets of cytoplasmic microBrown, 1973 ; Brown and Bouck, 1973) evidence tubules which (a) differ from each other in sensiwas presented that the characteristic asymmetric tivity to the microtubule depolymerizing agents shape of Ochromonas is mediated by two appar-colchicine and hydrostatic pressure, (b) are 514

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Vinblastine-induced paracrystals and unusually large microtubules (macrotubules) in rat renal cells

Cited by 51 publications

References 45 publications

Effect and Localization of Trifluralin in Plasmodium falciparum Gametocytes: An Electron Microscopic Study1

Effect and Localization of Trifluralin in Plasmodium falciparum Gametocytes: An Electron Microscopic Study1

Microtubules with 15 subunits in cockroach epidermal cells

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

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