Vimentin in Ependymal and Subventricular Proliferative Zone Cells of Rat Telencephalon

Abstract

Ependymal cells of cerebral ventricles are the most probable candidate progenitor cells. We studied ependymal and subventricular zone cells expressing intermediate filament protein vimentin. The results suggest that the ventricular ependyma represent a homogenous cell population. Some ependymocytes have long processes contacting with blood vessels, which makes them similar to tanycytes of the third cerebral ventricle. New data on the structure of ependymocyte processes attest to their active involvement into creation of a special microenvironment for neural stem cells of the subventricular proliferative zone.

Activated regulatory T cell regulates neural stem cell proliferation in the subventricular zone of normal and ischemic mouse brain through interleukin 10

“…Type B cells renew themselves and become type C and A cells (Gonzales-Roybal and Lim, 2013 ). Different cell types could be distinguished using different molecular markers (Ming and Song, 2005 ; Kirik and Korzhevskii, 2013 ): type A cells expressed DCX, PSA-NCAM, Tuj1 and Hu; type B cells expressed GFAP; type C cells expressed Mash1; and type E cells expressed Vimentin. We used DCX, GFAP, Mash1 and Vimentin staining and showed that activated Tregs injection increased BrdU + /Mash1 + cells in naïve mice, suggesting that Type C cells were the major target.…”

Concordant localization of functional urotensin II and urotensin II‐related peptide binding sites in the rat brain: Atypical occurrence close to the fourth ventricle

“…Vimentin is an intermediate filament protein highly expressed in ependymocytes of cerebral ventricles (Kirik and Korzhevskii, ). The vimentin antiserum (M0725, Dako) stained a single band of 57–58 kDa in western blot (manufacturer's technical information), and showed a pattern of staining similar to that described elsewhere using the same antibody (Kirik and Korzhevskii, ).…”

Immunohistochemical markers for neurobiology

“…В ряде исследований бывает необходимо провести селективное маркирование клеток, образующих выстилку желудочков мозга,эпендимоцитов и таницитов. В этом случае в качестве маркера может быть использован виментин [15]. Он относится к III классу ПФ и образует гомо-и гетеродимеры с другими белками ПФ -нестином и десмином.…”

“…Кроме того, наряду с нестином, виментин входит в состав цитоскелета нейральных стволовых и прогениторных клеток. В эмбриогенезе его обнаруживают в клетках радиальной глии [15]. При воздействии различного рода повреждающих факторов астроциты переходят в реактивное состояние и характеризуются, помимо гиперэкспрессии GFAP, экспрессией виментина (что в норме нехарактерно для астроцитов взрослого мозга млекопитающих).…”