Vimentin and glial fibrillary acidic protein are codistributed in the same intermediate filament system of malignant glioma cells in vivo

Paulus, Werner; Roggendorf, Wolfgang

doi:10.1007/bf02890003

Cited by 13 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently Wang et al (1984) using immunogold labeling and electron microscopy showed that in glioma cells in cultures the IF are vimentin and GFAP heteropolymers. These observations were recently confirmed by Paulus and Roggendorf (1988) on malignant glioma cells in situ.…”

Section: Introductionsupporting

confidence: 58%

Immuno‐electron microscopical localization of vimentin and glial fibrillary acidic protein in mouse astrocytes and their precursor cells in culture

Abd-El-Basset

Ahmed

Kalnins

et al. 1992

Glia

View full text Add to dashboard Cite

Immuno-electron microscopy was used to localize the distribution of vimentin and glial fibrillary acidic protein (GFAP) in mouse astrocytes and their precursor cells in primary cultures. In astroblasts and astrocytes, vimentin and GFAP form intermediate filaments (IF), which are heteropolymers, as previously observed in gliomas. Astrocytes and their precursor cells may have IF composed of GFAP-vimentin heteropolymer or vimentin alone, but IF composed of GFAP only were not seen. It seems that the formation of IF that are GFAP-vimentin heteropolymers is a feature of normal astroglia development and that the ratio of GFAP to vimentin in these IF reflects the degree of differentiation and functional state of the cell.

show abstract

Section: Introductionsupporting

confidence: 58%

Immuno‐electron microscopical localization of vimentin and glial fibrillary acidic protein in mouse astrocytes and their precursor cells in culture

Abd-El-Basset

Ahmed

Kalnins

et al. 1992

Glia

View full text Add to dashboard Cite

show abstract

“…As the cells of the astrocyte family mature, vimentin is gradually replaced by intermediate filaments composed of the more specific GFAP (Dahl, 1981;Pixley and De Vellis, 1984). In glial cultures (Chiu et al, 1981;Fedoroff, 1986) or under pathological conditions (Chiu et al, 1988;Paulus and Roggendorf, 1988;Pixley and De Vellis, 1984;Sharp et al, 1982), vimentin synthesis may persist or may be resumed, resulting in astrocytes that express both filament proteins simultaneously. It is therefore not surprising that our cultures contained both vimentin single-labeled and vimentidGFAP double-labeled cells and relatively large amounts of both proteins.…”

Section: Discussionmentioning

confidence: 99%

Region‐ and sex‐related differences in maturation of astrocytes in dissociated cell cultures of embryonic rat brain

Beyer

Epp

Fassberg

et al. 1990

Glia

View full text Add to dashboard Cite

Previous studies using dissociated cell cultures of fetal rat brain have revealed considerable regional diversity as well as sex steroid-independent sex differences in developmental schedules of dopaminergic neurons. Because these phenomena might be related to glial heterogeneity, cultures of dissociated male and female diencephalon, mesencephalon, and rhombencephalon of gestational day 14 rats were investigated with respect to the development of astrocytic markers. Cultures were incubated for 3-8 days in vitro (DIV) in serum-supplemented or serum-free medium. Vimentin and glial fibrillary acidic protein (GFAP) were quantified by counting of immunolabeled cells and immunoblotting. Vimentin and GFAP content rose from DIV 3 to 6 in all cultures. Regional variation of vimentin content was low, but large differences occurred in amounts of GFAP. GFAP reached high levels in rhombencephalon, especially when supplemented with serum, but remained very low or not detectable in mesencephalon. Simultaneous immunostaining for both cytoskeletal proteins revealed the presence of large numbers of vimentin single-labeled and small numbers of vimentin/GFAP double-labeled cells. Numbers of cells expressing GFAP showed similar regional variations as GFAP contents in both serum-free and serum-supplemented medium. They rose steeply from DIV 3 to 8 in rhomb- and diencephalon but not in mesencephalon. Transiently, female diencephalic cultures contained slightly more GFAP-immunoreactive cells than male cultures. The results thus demonstrate considerable regional heterogeneity of astrocytic maturation. However, neither the regional nor the sex differences show a consistent correlation with previous data on development of dopaminergic and other monoaminergic neurons in vitro. It seems likely that the dependence of neurons on glial environment for realization of an inherent developmental program varies among neuronal phenotypes.

show abstract

“…Nestin and synemin on the other hand are class IV IF proteins that cannot homodimerize and are characterized by an 8 to 10 times larger C-terminus. Heteropolymerisation of GFAP and vimentin was already discovered with immunoelectron microscopy in glioma cell lines and surgical biopsies over 30 years ago and more recently also nestin was observed to be part of the GFAP/vimentin-positive filaments in astrocytes Paulus and Roggendorf, 1988;Wang et al, 1984). Depletion of either vimentin or GFAP leads to different IF network structures in astrocytes, with GFAP dominant networks being more compact and dense, and vimentin dominant networks more dispersed (Lepekhin et al, 2001;Menet et al, 2001).…”

Section: The Gfap If Network Composition In Diffuse Gliomasmentioning

confidence: 93%

“…= long-term, S.T. = short-term (1) de Pablo et al (2013); (2) de Pascalis et al (2018); (3) Dupin et al (2011); (4) Elobeid et al (2000); (5) Moeton et al (2014); (6) Moeton et al (2016); (7) Murphy et al (1998); (8) Lepekhin et al (2001); (9) Pekny et al (1998); (10) Rutka et al (1994); (11) Rutka et al (1998); (12) Stassen et al (2017); (13) Toda et al (1994); (14) Toda et al (1999); (15) van Bodegraven et al (2019b); (16) Weinstein et al (1991); (17) Wilhelmsson et al (2003).…”

Section: The Functional Role Gfap and Gfap Isoforms In Glioma Cell Be...mentioning

confidence: 99%

GFAP Alternative Splicing and the Relevance for Disease – A Focus on Diffuse Gliomas

2022

View full text Add to dashboard Cite

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is characteristic for astrocytes and neural stem cells, and their malignant analogues in glioma. Since the discovery of the protein 50 years ago, multiple alternative splice variants of the GFAP gene have been discovered, leading to different GFAP isoforms. In this review, we will describe GFAP isoform expression from gene to protein to network, taking the canonical isoforms GFAPα and the main alternative variant GFAPδ as the starting point. We will discuss the relevance of studying GFAP and its isoforms in disease, with a specific focus on diffuse gliomas.

show abstract

Vimentin and glial fibrillary acidic protein are codistributed in the same intermediate filament system of malignant glioma cells in vivo

Cited by 13 publications

References 16 publications

Immuno‐electron microscopical localization of vimentin and glial fibrillary acidic protein in mouse astrocytes and their precursor cells in culture

Immuno‐electron microscopical localization of vimentin and glial fibrillary acidic protein in mouse astrocytes and their precursor cells in culture

Region‐ and sex‐related differences in maturation of astrocytes in dissociated cell cultures of embryonic rat brain

GFAP Alternative Splicing and the Relevance for Disease – A Focus on Diffuse Gliomas

Contact Info

Product

Resources

About