Vigorous, but differential mononuclear cell response of cirrhotic patients to bacterial ligands

Barbero-Becerra, Varenka J.; Gutiérrez‐Ruiz, María Concepción; Maldonado‐Bernal, Carmen; Téllez-Ávila, Félix Ignacio; Alfaro-Lara, Roberto; Vargas-Voráckóva, Florencia

doi:10.3748/wjg.v17.i10.1317

Cited by 4 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is reasonable, as from the host's point of a view an invading microorganism is simply undesirable, whatever its reaction to the Gram stain. Indeed, modulo a few detailed differences [30], and certainly for our present purposes, it seems that LTA is indeed broadly equivalent to LPS in terms of its ability to stimulate an innate immune response [31][32][33].…”

Section: Introductionmentioning

confidence: 78%

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™ stains

Pretorius

Pagé

Hendricks

et al. 2018

J. R. Soc. Interface.

View full text Add to dashboard Cite

In recent work, we discovered that the presence of highly substoichiometric amounts (10−8 molar ratio) of lipopolysaccharide (LPS) from Gram-negative bacteria caused fibrinogen clotting to lead to the formation of an amyloid form of fibrin. We here show that the broadly equivalent lipoteichoic acids (LTAs) from two species of Gram-positive bacteria have similarly (if not more) potent effects. Using thioflavin T fluorescence to detect amyloid as before, the addition of low concentrations of free ferric ion is found to have similar effects. Luminescent conjugated oligothiophene dyes (LCOs), marketed under the trade name Amytracker™, also stain classical amyloid structures. We here show that they too give very large fluorescence enhancements when clotting is initiated in the presence of the four amyloidogens (LPS, ferric ions and two LTA types). The staining patterns differ significantly as a function of both the amyloidogens and the dyes used to assess them, indicating clearly that the nature of the clots formed is different. This is also the case when clotting is measured viscometrically using thromboelastography. Overall, the data provide further evidence for an important role of bacterial cell wall products in the various coagulopathies that are observable in chronic, inflammatory diseases. The assays may have potential in both diagnostics and therapeutics.

show abstract

Section: Introductionmentioning

confidence: 78%

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™ stains

Pretorius

Pagé

Hendricks

et al. 2018

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…LTA species have been rather less studied from the point of view of inflammagenesis than have LPS forms, but they clearly reside in the blood and are inflammagens (Barbero‐Becerra et al ., ; Cinar et al ., ; Hoogerwerf et al ., ; Levels et al ., ; Pirillo, Catapano & Norata, ). In some respects (see Section X), LTAs may be even more potent than LPS species (Pretorius et al ., ).…”

Section: Step 4: Microbes Can Produce and Shed Inflammagens Such As Lmentioning

confidence: 98%

No effects without causes: the Iron Dysregulation and Dormant Microbes hypothesis for chronic, inflammatory diseases

Kell

2018

Biological Reviews

108

View full text Add to dashboard Cite

Since the successful conquest of many acute, communicable (infectious) diseases through the use of vaccines and antibiotics, the currently most prevalent diseases are chronic and progressive in nature, and are all accompanied by inflammation. These diseases include neurodegenerative (e.g. Alzheimer's, Parkinson's), vascular (e.g. atherosclerosis, pre‐eclampsia, type 2 diabetes) and autoimmune (e.g. rheumatoid arthritis and multiple sclerosis) diseases that may appear to have little in common. In fact they all share significant features, in particular chronic inflammation and its attendant inflammatory cytokines. Such effects do not happen without underlying and initially ‘external’ causes, and it is of interest to seek these causes. Taking a systems approach, we argue that these causes include (i) stress‐induced iron dysregulation, and (ii) its ability to awaken dormant, non‐replicating microbes with which the host has become infected. Other external causes may be dietary. Such microbes are capable of shedding small, but functionally significant amounts of highly inflammagenic molecules such as lipopolysaccharide and lipoteichoic acid. Sequelae include significant coagulopathies, not least the recently discovered amyloidogenic clotting of blood, leading to cell death and the release of further inflammagens. The extensive evidence discussed here implies, as was found with ulcers, that almost all chronic, infectious diseases do in fact harbour a microbial component. What differs is simply the microbes and the anatomical location from and at which they exert damage. This analysis offers novel avenues for diagnosis and treatment.

show abstract

Primary prophylaxis with ciprofloxacin in cirrhotic patients with ascites: a randomized, double blind study

Téllez-Ávila

Sifuentes–Osornio

Barbero-Becerra

et al. 2014

Annals of Hepatology

View full text Add to dashboard Cite

Vigorous, but differential mononuclear cell response of cirrhotic patients to bacterial ligands

Abstract: Peripheral blood mononuclear cells of cirrhotic patients are able to respond to a sudden bacterial ligand exposure, particularly lipopolysaccharide, suggesting that immune regulation mechanisms are still present.

Cited by 4 publications

References 29 publications

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™ stains

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™ stains

No effects without causes: the Iron Dysregulation and Dormant Microbes hypothesis for chronic, inflammatory diseases

Primary prophylaxis with ciprofloxacin in cirrhotic patients with ascites: a randomized, double blind study

Contact Info

Product

Resources

About