Vigna spp. Root-Nodules Harbor Potentially Pathogenic Fungi Controlled By Co-habiting Bacteria

Silva, Valéria Borges da; Bomfim, Cláudia Silva Gomes; Sena, Pâmella Thalita Souza; Santos, Jéssica Caroline Souza; Mattos, Wesley da Silva; Gava, C. A. T.; Souza, Adaílson Pereira de; Fernandes‐Júnior, Paulo Ivan

doi:10.1007/s00284-021-02455-3

Cited by 8 publications

(5 citation statements)

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“…These fungi, identified across all Desmodium species, colonize root-nodules by developing abruptly ending, thin-walled hyphae within cortical cells (Russell et al, 2002 ). Notably, pathogenic and non-pathogenic fungi have been identified in asymptomatic legumes, with potential mitigating effects by cohabiting bacteria (Da Silva et al, 2021 ). Talaromyces spp.…”

Section: Discussionmentioning

confidence: 99%

“…After that, they were sterilized by washing with 70% ethanol and 1% sodium hypochlorite for 2 min each to remove air bubbles from the tissues and reduce surface tension. This was followed by six-time washes with sterile distilled water, as previously described by Da Silva et al ( 2021 ). To validate sterility, the distilled water from the last rinsing step was plated on yeast extract mannitol agar (YEMA) and potato dextrose agar (PDA) (Oxoid Ltd, Basingstoke Hampshire RG24, UK), and incubated at 28 ± 2°C for 48 h and 25 ± 2°C for 72 h for bacteria and fungi, respectively (Vincent, 1970 ; Jing et al, 2022 ; Jalloh et al, 2024 ).…”

Section: Methodsmentioning

confidence: 99%

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Comparative microbiome diversity in root-nodules of three Desmodium species used in push-pull cropping system

Adan,

Asudi,

Niassy

et al. 2024

Front. Microbiol.

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BackgroundDesmodium species used as intercrops in push-pull cropping systems are known to repel insect-pests, suppress Striga species weeds, and shift soil microbiome. However, the mechanisms through which Desmodium species impact the soil microbiome, either through its root exudates, changes in soil nutrition, or shading microbes from its nodules into the rhizosphere, are less understood. Here, we investigated the diversity of root-nodule microbial communities of three Desmodium species- Desmodium uncinatum (SLD), Desmodium intortum (GLD), and Desmodium incanum (AID) which are currently used in smallholder maize push-pull technology (PPT).MethodsDesmodium species root-nodule samples were collected from selected smallholder farms in western Kenya, and genomic DNA was extracted from the root-nodules. The amplicons underwent paired-end Illumina sequencing to assess bacterial and fungal populations.ResultsWe found no significant differences in composition and relative abundance of bacterial and fungal species within the root-nodules of the three Desmodium species. While a more pronounced shift was observed for fungal community compositions compared to bacteria, no significant differences were observed in the general diversity (evenness and richness) of fungal and bacterial populations among the three Desmodium species. Similarly, beta diversity was not significantly different among the three Desmodium species. The root-nodule microbiome of the three Desmodium species was dominated by Bradyrhizobium and Fusarium species. Nevertheless, there were significant differences in the proportion of marker gene sequences responsible for energy and amino acid biosynthesis among the three Desmodium species, with higher sequence proportions observed in SLD.ConclusionThere is no significant difference in the microbial community of the three Desmodium species used in PPT. However, root-nodule microbiome of SLD had significantly higher marker gene sequences responsible for energy and amino acid biosynthesis. Therefore, it is likely that the root-nodules of the three Desmodium species host similar microbiomes and influence soil health, consequently impacting plant growth and agroecosystem functioning.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comparative microbiome diversity in root-nodules of three Desmodium species used in push-pull cropping system

Adan,

Asudi,

Niassy

et al. 2024

Front. Microbiol.

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“…Copwea is affected by many diseases, such as root rot and damping off, as these diseases cause losses that may reach 55% of the cowpea production 4,5 . Moreover, the losses can get 100% yield in the appropriate environmental conditions 6,7 . Root and stem rot disease is caused by fungi such as Fusarium solani and Rhizoctonia solani, the most dangerous fungi in cowpea 8,9 , because they contain toxins and enzymes (fludarabine, fusaric acid, javanicine, pectin methyl esterase and galacturonic enzymes), as well as their ability to tolerate toxins and antibiotics secreted by other organisms 10,11 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and diagnosis of some associated fungi with cowpea root rot disease and testing its pathogenicity

Awad

Farhan

2023

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Execute search by date 1/4/2021, The results of collecting samples from the regions of Anbar, Baghdad, Salah al-Din and Wasit showed that cowpea root rot disease is widespread in all studied areas, and The results of isolation phenotypic and molecular diagnosis showed the presence of different isolation of fungi that infected cowpea root, such as Fusarium nygamai (Fu1), F. nygamai (Fu2), F. solani (Fu3), F. solani(Fu4), Rhizoctonia solani (Rh5), and Fusarium solani (Fu6), The results of the pathogenicity test on radish seeds showed that tested isolates were significantly decreased germination percentage of radish seeds of water Ager, and the most effective isolation was F. solani (Fu4). The infection rate was 90% compared to 0.00% of the control media treatment, which was uncontaminated by the pathogenic fungus. Isolated fungi showed a difference in the percentage and severity of infection on cowpea seedlings and seedlings, as the isolate of F.nygamai (Fu1) achieved the highest infection rate of 66.67 % and the severity of disease at 75%. All fungal isolates significantly increased the rate and severity of infection on seed radish compared with the control treatment not contaminated with pathogenic fungi by 0.0%. Keywords: Vegan unguiculata; Rhizoctonia solani; and Fusarium solani; PCR.

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“…These fungi, identified across all Desmodium species, colonize root-nodules by developing abruptly ending, thin-walled hyphae within cortical cells (Russell et al, 2002). Notably, pathogenic and non-pathogenic fungi have been identified in asymptomatic legumes, with potential mitigating effects by cohabiting bacteria (Da Silva et al, 2021). Talaromyces spp.…”

Section: Discussionmentioning

confidence: 99%

“…After that, they were sterilized by washing with 70% ethanol and 1% sodium hypochlorite for 2 min each to remove air bubbles from the tissues and reduce surface tension. This was followed by six-time washes with sterile distilled water, as previously described by Da Silva et al (2021). To validate sterility, the distilled water from the last rinsing step was plated on yeast extract mannitol agar (YEMA) and potato dextrose agar (PDA) (Oxoid Ltd, Basingstoke Hampshire RG24, UK), and incubated at 28 ± 2 • C for 48 h and 25 ± 2 • C for 72 h for bacteria and fungi, respectively (Vincent, 1970;Jing et al, 2022;Jalloh et al, 2024).…”

Section: Sample Collectionmentioning

confidence: 99%

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Vigna spp. Root-Nodules Harbor Potentially Pathogenic Fungi Controlled By Co-habiting Bacteria

Cited by 8 publications

References 48 publications

Comparative microbiome diversity in root-nodules of three Desmodium species used in push-pull cropping system

Comparative microbiome diversity in root-nodules of three Desmodium species used in push-pull cropping system

Isolation and diagnosis of some associated fungi with cowpea root rot disease and testing its pathogenicity

Table_2.DOCX

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