Vigilin interacts with CTCF and is involved in the maintenance of imprinting of IGF2 through a novel RNA–mediated mechanism

Yu, Xiaoqin; Liu, Qiuying; He, Jinyang; Huang, Yuan; Jiang, Lei; Xie, Xiaoyan; Liu, Ji; Wei, Ling; Qin, Yang

doi:10.1016/j.ijbiomac.2017.11.109

Cited by 9 publications

(7 citation statements)

References 30 publications

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“…As this domain binds to RNA, further investigations indicated that the vigilin-CTCF interactions are RNAdependent and mediated by the lncRNA H19. The H19 knock-down results in an alteration of the IGF2 imprinting pattern, supporting the idea that the CTCF-based imprinting also depends on the interactions between vigilin and H19 lncRNA [126].…”

Section: Ctcf Involvement In Imprinting-related Diseasessupporting

confidence: 64%

One protein to rule them all: The role of CCCTC-binding factor in shaping human genome in health and disease

Łażniewski

Dawson

Rusek

et al. 2019

Seminars in Cell & Developmental Biology

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The eukaryotic genome, constituting several billion base pairs, must be contracted to fit within the volume of a nucleus where the diameter is on the scale of µm. The 3D structure and packing of such a long sequence, cannot be left to pure chance, as DNA must be efficiently used for its primary roles as a matrix for transcription and replication. In recent years, methods like chromatin conformation capture (including 3C, 4C, Hi-C, ChIA-PET and Multi-ChIA) and optical microscopy have advanced substantially and have shed new light on how eukaryotic genomes are hierarchically organized; first into 10-nm fiber, next into DNA loops, topologically associated domains and finally into interphase or mitotic chromosomes. This knowledge has allowed us to revise our understanding regarding the mechanisms governing the process of DNA organization. Mounting experimental evidence suggests that the key element in the formation of loops is the binding of the CCCTC-binding factor (CTCF) to DNA; a protein that can be referred to as the chief organizer of the genome. However, CTCF does not work alone but in cooperation with other proteins, such as cohesin or Yin Yang 1 (YY1). In this short review, we briefly describe our current understanding of the structure of eukaryotic genomes, how they are established and how the formation of DNA loops can influence gene expression. We discuss the recent discoveries describing the 3D structure of the CTCF-DNA complex and the role of CTCF in establishing genome structure. Finally, we briefly explain how various genetic disorders might arise as a consequence of mutations in the CTCF target sequence or alteration of genomic imprinting.

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Section: Ctcf Involvement In Imprinting-related Diseasessupporting

confidence: 64%

One protein to rule them all: The role of CCCTC-binding factor in shaping human genome in health and disease

Łażniewski

Dawson

Rusek

et al. 2019

Seminars in Cell & Developmental Biology

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“…Future studies have to reveal if and how IGF2BP1 influences other HDLBP-dependent regulation of gene expression. Of particular interest in this respect are recent reports suggesting that HDLBP influences IGF2 synthesis by modulating imprinting via association with CTCF and the non-coding H19 RNA (Yu et al, 2018), a reported target RNA of IGF2BP1 in cancer cells, reviewed in Zhang et al (2017). A novel finding of our studies is an apparently conserved role of IGF2BP1 in modulating the unfolded protein response in cancer cells.…”

Section: Validation Of Novel Igf2bp1 Targetsmentioning

confidence: 66%

IGF2BP1, a Conserved Regulator of RNA Turnover in Cancer

Glaß

Misiak

Bley

et al. 2021

Front. Mol. Biosci.

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The oncofetal IGF2 mRNA-binding protein 1 (IGF2BP1) promotes tumor progression in a variety of solid tumors and its expression is associated with adverse prognosis. The main role proposed for IGF2BP1 in cancer cells is the stabilization of mRNAs encoding pro-oncogenic factors. Several IGF2BP1-RNA association studies, however, revealed a plethora of putative IGF2BP1-RNA targets. Thus, at present the main conserved target RNAs and pathways controlled by IGF2BP1 in cancer remain elusive. In this study, we present a set of genes and cancer hallmark pathways showing a conserved pattern of deregulation in dependence of IGF2BP1 expression in cancer cell lines. By the integrative analysis of these findings with publicly available cancer transcriptome and IGF2BP1-RNA association data, we compiled a set of prime candidate target mRNAs. These analyses confirm a pivotal role of IGF2BP1 in controlling cancer cell cycle progression and reveal novel cancer hallmark pathways influenced by IGF2BP1. For three novel target mRNAs identified by these studies, namely AURKA, HDLBP and YWHAZ, we confirm IGF2BP1 mRNA stabilization. In sum our findings confirm and expand previous findings on the pivotal role of IGF2BP1 in promoting oncogenic gene expression by stabilizing target mRNAs in a mainly 3’UTR, m6A-, miRNA-, and potentially AU-rich element dependent manner.

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“…Additionally, interaction of CTCF with variant DNA sequences diversely changes the conformation of CTCF, and these conformational changes likely influence its interaction with diverse nuclear proteins [22]. This mechanism, whereby the differentially combinatorial zinc-finger binding to diverse sequences may facilitate engagement with various binding partners [16][17][18][23][24][25][26][27][28], diverse post-translational modifications, and ultimately confer CTCF with multiple functional roles, emphasizes the versatile functions of CTCF. We will first briefly introduce the classical roles of CTCF, before reviewing the current information about its role in guarding genome integrity.…”

Section: The Multifunctional Ctcf Protein and Its Classical Rolesmentioning

confidence: 99%

“…Regulation of insulator functions [23,24] Suz12 Regulation of insulator function (H19 ICR)Transcriptional repression of Sox2 [23,24] Sin3A Transcriptional repression via recruitment of HDAC [23,24] Taf-1/Set Unknown [23,24] H2A/H2A.Z Co-localize genome-wide [23,24] HP1α Interact in pericentric heterochromatin (PCH) and restricts H4K20me3 and H3K27me3 distribution [26] Genome integrity PARP1 Regulation of crosstalk between poly(ADP-ribosyl)ation and DNA methylation [23,24] RNA binding protein Vigilin Interacts with CTCF via H19 lncRNA and keep the imprinting of IGF2 [27] Nuclear architecture…”

Section: Chd8mentioning

confidence: 99%

A Newly Assigned Role of CTCF in Cellular Response to Broken DNAs

Kang

Lee

2021

Biomolecules

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Best known as a transcriptional factor, CCCTC-binding factor (CTCF) is a highly conserved multifunctional DNA-binding protein with 11 zinc fingers. It functions in diverse genomic processes, including transcriptional activation/repression, insulation, genome imprinting and three-dimensional genome organization. A big surprise has recently emerged with the identification of CTCF engaging in the repair of DNA double-strand breaks (DSBs) and in the maintenance of genome fidelity. This discovery now adds a new dimension to the multifaceted attributes of this protein. CTCF facilitates the most accurate DSB repair via homologous recombination (HR) that occurs through an elaborate pathway, which entails a chain of timely assembly/disassembly of various HR-repair complexes and chromatin modifications and coordinates multistep HR processes to faithfully restore the original DNA sequences of broken DNA sites. Understanding the functional crosstalks between CTCF and other HR factors will illuminate the molecular basis of various human diseases that range from developmental disorders to cancer and arise from impaired repair. Such knowledge will also help understand the molecular mechanisms underlying the diverse functions of CTCF in genome biology. In this review, we discuss the recent advances regarding this newly assigned versatile role of CTCF and the mechanism whereby CTCF functions in DSB repair.

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Vigilin interacts with CTCF and is involved in the maintenance of imprinting of IGF2 through a novel RNA–mediated mechanism

Cited by 9 publications

References 30 publications

One protein to rule them all: The role of CCCTC-binding factor in shaping human genome in health and disease

One protein to rule them all: The role of CCCTC-binding factor in shaping human genome in health and disease

IGF2BP1, a Conserved Regulator of RNA Turnover in Cancer

A Newly Assigned Role of CTCF in Cellular Response to Broken DNAs

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