Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation

Cohen, Jacques; Inge, K. L.; Suzman, Michael; Wiker, Sharon R.; Wright, Graham

doi:10.1016/s0015-0282(16)60673-8

Cited by 132 publications

(57 citation statements)

References 10 publications

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“…Cohen etal. (10) found that ZPTV, not the thickness per se, was a strong predictor of IVF treatment outcome. However, the impact of this parameter has so far not been confirmed.…”

Section: Introductionmentioning

confidence: 83%

See 1 more Smart Citation

Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment

Palmstierna

Murkes

Csemiczky

et al. 1998

J Assist Reprod Genet

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“…Cohen etal. (10) found that ZPTV, not the thickness per se, was a strong predictor of IVF treatment outcome. However, the impact of this parameter has so far not been confirmed.…”

Section: Introductionmentioning

confidence: 83%

“…A selection based on EDR and fragmentation (5) was already performed when choosing the preembryos for ER. ZPTV was determined by direct measurement of the zona pellucida thickness on the video screen by a ruler at three locations and then calculated as indicated in Table II (10).…”

Section: Videocinematography and Morphologic Assessmentmentioning

confidence: 99%

Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment

Palmstierna

Murkes

Csemiczky

et al. 1998

J Assist Reprod Genet

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“…It is to be pointed out, however, that an apparent discrepancy about the average ZPT values exists among different works. This could be due to several factors including different scoring methods, high variability oocytes shape among patients and different culture conditions [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Correlation of oocyte morphometry parameters with woman’s age

Valeri

Pappalardo

Felici

et al. 2011

J Assist Reprod Genet

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Purpose Aim of this study was to evaluate morphometric parameters of metaphase II oocytes, including cytoplasm diameter (CD), zona pellucida thickness (ZPT) and width of the perivitelline space (PS), in relation with zona pellucida birefringence, spindle presence and age of the woman. Methods Oocytes were classified into groups according to zona birefringence (low or high zona birefringence, LZB and HZB, respectively) and presence or absence of a visible spindle (SP and aSP, respectively).Results HZB oocytes showed a thicker zona (17.7±0.3 μm) than LZB oocytes (16.7±0.3 μm, p<0.01). Moreover, PS was narrower in HZB and SP oocytes than in LZB (p< 0,001) and aSP (p<0,05) oocytes. Finally, we found that CD and ZPT linearly decrease with age of the woman (CD r= 0.028: p<0.01; ZPT r=0.050: p<0.0001). Conclusion Our results evidence an association in human oocytes between zona pellucida and spindle birefringence and defined morphometric parameters and a decrease of oocyte size and ZPT as a function of women's age.

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“…The rationale for assisted hatching An important observation leading to the clinical introduction of assisted hatching was the finding that microsurgically fertilised embryos with artificial gaps in their zona appear to have high rates of implantation [34], and that cleaved embryos with a good prognosis for implantation have reduced zona thickness [35].…”

Section: Hatching Processmentioning

confidence: 99%

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

105

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The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

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Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation

Cited by 132 publications

References 10 publications

Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment

Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment

Correlation of oocyte morphometry parameters with woman’s age

Assisted hatching in assisted reproduction: a state of the art

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