Vidas UP–Enzyme-Linked Fluorescent Immunoassay Based on Recombinant Phage Protein and FluorescenceIn SituHybridization as Alternative Methods for Detection ofSalmonella entericaSerovars in Meat

Abstract

Several methods for the rapid and specific detection of Salmonella spp. in meat have been described. This study was conducted to evaluate the capability of the VIDAS(®) UP (SPT [Salmonella Phage Technology]), an enzyme-linked fluorescent immunoassay method, and fluorescence in situ hybridization (FISH) to complement the International Organization for Standardization Method 6579 (ISO) in detecting Salmonella spp. from beef, pork, and poultry meat samples. The meat was inoculated with a mixture of Salmonella spp. on three levels of contamination. It was also checked that the tests did not produce cross-reactions with other Enterobacteriaceae rods. On the basis of the results, the relative specificity, relative accordance, and relative sensitivity of the method were determined. In meat samples, Vidas UP and FISH detection results were in substantial agreement with ISO, with relative specificity, accordance, and sensitivity rates of 90%, 96.3%, and 100%, respectively, for Vidas UP and 100%, 100%, and 99.4%, respectively, for FISH. This is the first report on the evaluation of both Vidas UP and FISH compared to ISO for the rapid detection of Salmonella enterica serovars in meat.

High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods

“…The results showed that FISH probes represents an effective tool for detecting and enumerating pathogens in wastewater due to its efficiency, specifity and sensivity, even when viable but non-culturable (VBNC) cells are present. FISH method has been previously used with good results for identification of Salmonella in clinical samples (Nordentoft et al, 1997;O'Keefe et al, 2001;Frickman et al, 2013) or in artificially inoculated environmental samples (Zadernowska et al, 2014;Sha et al, 2013). Almeida et al (2010; reported the use of a PNA FISH probe to detect Salmonella in natural fountain water and biofilms.…”

“…The FISH assay is less sensitive to inhibitory substances than PCR and has shown to be a very useful tool for phylogenetic, ecological, diagnostic and environmental microbiology studies (Bottari et al, 2006). It has been successfully used for detection and identification of different pathogens, including Salmonella, in foods, surface water, drinking water and wastewater (Zadernowska et al, 2014;Sha et al, 2013;Almeida et al, 2011;Almeida et al, 2010;Gironés et al, 2010).…”

Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis

“…Numerous alternative methodologies exist for the rapid analysis of foodborne pathogens, including immunoassays (e.g. automated Vidas [4,5] and lateral flow immunochromatography [6][7][8]), and quantitative PCR (qPCR) (e.g.…”