Vibriocidal antibodies induced byYersinia enterocoliticaserotype IX

Abstract

SUMMARYYersinia enterocolitica serotype IX has been found to stimulate the production of vibriocidal antibody against Vibrio cholerae, particularly in Inaba serotype, in experimental rabbits and infected man to a significant degree. This activity could be absorbed by both Inaba and Yersinia antigens from anti-yersinia sera, but Yersinia antigen could not absorb vibriocidal activity from the anti-cholera sera, indicating a unilateral relation.Vibrio agglutinating antibody, particularly against Ogawa, in anti-yersinia rabbit or human sera was found to be less liable to non-specific stimulation.V. cholerae, while removing vibriocidal antibody, could not absorb the Yersinia agglutinin from anti-yersinia sera, suggesting that there is a different determinant for these two types of antibody activities. The non-specific production of vibriocidal antibody by Y. enterocolitica type IX indicates the need for caution in the interpretation of the results of vibriocidal tests where such organisms are prevalent.

Structure of the O‐chain of the phenol‐phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9

“…

The phcnol-phase soluble cellular lipopolysaccharide isolated by the phenol/water extraction method from Yersinia enterocolitica serotpye 0 : 9 cells was shown by hydrolytic, periodate oxidation, methylation and nuclear magnetic resonance studies to be an S-type lipopolysaccharide with a linear 0-antigenic polysaccharide of 1,Z-linked 4,h-dideoxy-Cformamido-x-r~-mannopyranosyl units. The serological cross-reactivity between Y. enterocolitica serotype 0 : 9 and the lipopolysaccharides of Vibrio cholerue and Brucella species can now be related to the presence of N-acylated 4-amino-4,h-dideoxy-a-r~mannopyranosyl residues in their respective 0-antigenic chains.Cross-serological reactions between Yersinia enterocolitica serotype 0 : 9 and Brucella abortus antigens have been demonstrated using human sera [I, 21 and the reactions have been confirmed with other antisera [3 -71 and have been shown to extend to other Brucella species [8] and also to Vibrio cholerae antigens [9]. Sandulache and Marx [lo] showed that the hot phenol/water extraction [l 11 of Y. enterocolitica serotype 0 : 9 cells afforded two distinct lipopolysaccharidcs (LPS), one being found in the aqueous phase and the other in the phenol phase.

…”

Faecal carriage of klebsiella by patients with ankylosing spondylitis and rheumatoid arthritis.

“…Yersinia pseudotuberculosis is not known to provoke reactive arthritis more commonly in individuals with HLA B27, though a small number of cases have been recorded (Ahvonen, 1972;Chambers et al, 1978). Yersinia enterocolitica serotype 9 is known to cross-react with Vibrio cholerae (Barua and Watanabe, 1972), and 0 antigenic relationships between Salmonella, V. cholerae, and Escherichia coli are described (Winkle et al, 1972). Yersinia enterocolitica serotype 012 cross-reacts with Salmonella 0 antigen 047 (Wauters et al, 1971).…”

“…Yersinia enterocolitica serotype 9 is known to cross-react with Vibrio cholerae (Barua and Watanabe, 1972), and 0 antigenic relationships between Salmonella, V. cholerae, and Escherichia coli are described (Winkle et al, 1972). Yersinia enterocolitica serotype 012 cross-reacts with Salmonella 0 antigen 047 (Wauters et al, 1971).…”

Significância de anticorpos vibriocidas circulantes em área pós-epidêmica de diarréia, São Bento do Una, Estado de Pernambuco

“…Isso decorre da propriedade que os títulos dos anticorpos vibriocidas persistem por 6 a 12 meses com retorno a níveis anteriores à infecção após 13 a 19 meses 3 6 9 . É interessante que nas áreas não endêmicas, pequena parcela da população (< 15%, em todas as idades) apresentaram anticorpos vibriocidas naturais, provavelmente provenientes da exposição de outros microrganismos (Brucella e Enterobactérias) 1 8 UFC/mL foram misturadas a um excesso de complemento (Sigma). A adição dessa mistura as cavidades da placa com os soros, em volumes iguais, determinou a duplicação dos títulos iniciais para 1:10 a 1:5120.…”

“…Ainda como controles foram incluídos um anti-soro O 1 Outras explicações podem ser aduzidas como a presença da fração antigênica C, na variação entre Ogawa e Inaba, tendo em vista que Sakazaki & Tamura 16 , demonstraram que o sorovar Inaba descendia de Ogawa após a perda da fração B. A reversão inversa não ocorre, isto é, Inaba para Ogawa.…”