Vibrio tetraodonis subsp. pristinus subsp. nov., isolated from the coral Acropora cytherea at Palmyra Atoll, and creation and emended description of Vibrio tetraodonis subsp. tetraodonis subsp. nov

Loughran, Rachel M.; Emsley, Sarah A; Jefferson, Tori; Wasson, Benjamin J.; Deadmond, Monica C.; Knauss, Taylor L.; Pfannmuller, Kaysa M.; Lippert, Katherine J.; Miller, Gregory; Cline, Lauren C.; Oline, David K.; Koyack, Marc J.; Grant-Beurmann, Silvia; Gaylor, Michael O.; Saw, Jimmy H.; Ushijima, Blake; Videau, Patrick

doi:10.1007/s10482-022-01766-0

Cited by 3 publications

(3 citation statements)

References 65 publications

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“…Finally, our analysis did not identify bacterial strains harbouring more than one T3SS2 gene cluster within their genomes. Interestingly, several of these T3SS2s were identified in recently validated or proposed Vibrio and Shewanella species, including Vibrio paracholerae [ 69 ], Vibrio tarriae [ 70 ], ' Vibrio tetraodonis' [ 71 ], Shewanella oncorhynchi [ 72 ] and ' Shewanella psychropiezotolerans' [ 73 ] ( Fig. 2 , Table S1).…”

Section: Resultsmentioning

confidence: 99%

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

et al. 2023

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Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. A distinctive feature of the O3:K6 pandemic clone, and its derivatives, is the presence of a second, phylogenetically distinct, type III secretion system (T3SS2) encoded within the genomic island VPaI-7. The T3SS2 allows the delivery of effector proteins directly into the cytosol of infected eukaryotic cells to subvert key host-cell processes, critical for V. parahaemolyticus to colonize and cause disease. Furthermore, the T3SS2 also increases the environmental fitness of V. parahaemolyticus in its interaction with bacterivorous protists; hence, it has been proposed that it contributed to the global oceanic spread of the pandemic clone. Several reports have identified T3SS2-related genes in Vibrio and non- Vibrio species, suggesting that the T3SS2 gene cluster is not restricted to the Vibrionaceae and can mobilize through horizontal gene transfer events. In this work, we performed a large-scale genomic analysis to determine the phylogenetic distribution of the T3SS2 gene cluster and its repertoire of effector proteins. We identified putative T3SS2 gene clusters in 1130 bacterial genomes from 8 bacterial genera, 5 bacterial families and 47 bacterial species. A hierarchical clustering analysis allowed us to define six T3SS2 subgroups (I–VI) with different repertoires of effector proteins, redefining the concepts of T3SS2 core and accessory effector proteins. Finally, we identified a subset of the T3SS2 gene clusters (subgroup VI) that lacks most T3SS2 effector proteins described to date and provided a list of 10 novel effector candidates for this subgroup through bioinformatic analysis. Collectively, our findings indicate that the T3SS2 extends beyond the family Vibrionaceae and suggest that different effector protein repertories could have a differential impact on the pathogenic potential and environmental fitness of each bacterium that has acquired the Vibrio T3SS2 gene cluster.

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Section: Resultsmentioning

confidence: 99%

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

et al. 2023

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“…Finally, our analysis did not identify bacterial strains harboring more than one T3SS2 gene cluster within their genomes. Interestingly, several of these T3SS2 were identified in recently isolated and/or classified Vibrio and Shewanella species, including V. paracholerae [74], V. tarriae [75], V. tetraodonis [76], S. oncorhynchi [77] and S. psychropiezotolerans [78] ( Fig. 2 and Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

TheVibrioType III Secretion System 2 is not restricted to theVibrionaceaeand encodes differentially distributed repertoires of effector proteins

Jerez

Plaza

Bravo

et al. 2022

Preprint

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Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. A distinctive feature of the O3:K6 pandemic clone, and its derivatives, is the presence of a second, phylogenetically distinct, Type III Secretion System (T3SS2) encoded within the genomic island VPaI-7. The T3SS2 allows the delivery of effector proteins directly into the cytosol of infected eukaryotic cells to subvert key host cell processes, critical for V. parahaemolyticus to colonize and cause disease. Furthermore, the T3SS2 also increases the environmental fitness of V. parahaemolyticus in its interaction with bacterivorous protists; hence it has been proposed that it contributed to the global oceanic spread of the pandemic clone. Several reports have identified T3SS2-related genes in Vibrio and non-Vibrio species, suggesting that the T3SS2 gene cluster is not restricted to the Vibrionaceae and can mobilize through horizontal gene transfer events. In this work, we performed a large-scale genomic analysis to determine the phylogenetic distribution of the T3SS2 gene cluster and its repertoire of effector proteins. We identified putative T3SS2 gene clusters in 1130 bacterial genomes from eight bacterial genera and five bacterial families. A hierarchical clustering analysis allowed us to define six T3SS2 subgroups (I-VI) with different repertoires of T3SS2 effector proteins, redefining the concepts of T3SS2 core and accessory effector proteins. Finally, we identified a subset of T3SS2 gene clusters that lack most T3SS2 effector proteins described to date and provided a list of 10 novel effector candidates for this subgroup through bioinformatic analysis. Collectively, our findings indicate that the T3SS2 extends beyond the Vibrionaceae family and suggest that different effector protein repertories could have a differential impact on the pathogenic potential and environmental fitness of all the bacteria that have acquired the Vibrio T3SS2 gene cluster.

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“…Some Vibrio cause disease in animals and humans, such as Vibrio cholerae, Vibrio mimicus, which causes gastroenteritis, and Vibrio vulnificus which causes parenteral infections in humans (Jing et al, 2020). In recent years, due to the characteristic ecological and physiological property, more and more marine Vibrio have been isolated (Loughran et al, 2022;Fahmy, 2022). Xu et al isolated a novel Vibrio strain in 2009, named Vibrio hangzhouensis from sediments of the East China Sea.…”

Section: Introductionmentioning

confidence: 99%

Characterization and genome analysis of Vibrio phage vB_VhaP_PG11, representing a new viral genus

Jing

Guo²,

Wang³

et al. 2023

Front. Mar. Sci.

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Vibrio is a kind of common gram-negative bacteria, which is widely distributed in marine and estuarine environments. In the study, a novel marine phage vB_VhaP_PG11, infecting Vibrio hangzhouensis, was isolated from the offshore waters of Qingdao, China. vB_VhaP_PG11 is a double-stranded DNA phage. The whole genome proteomic tree shows that vB_VhaP_PG11 phage is related to two Vibrio phages, Vibrio phage 1.238.A._10N.261.52.F10 and Vibrio phage 1.245.O._10N.261.54.C7, but with low homology. Their amino acids identity with vB_VhaP_PG11 is 42.77 and 41.49% respectively. The prediction results of genome-blast distance phylogeny (GBDP) and the analysis gene-sharing network indicate that vB_VhaP_PG11 belongs to a new genus in Schitoviridae, named Qingschitovirus. The study of Vibrio phage vB_VhaP_PG11 provides basic information contributing to a better understanding of interactions between Vibrio phages and their hosts and helps analyze unknown viral sequences in the metagenomic database.

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Vibrio tetraodonis subsp. pristinus subsp. nov., isolated from the coral Acropora cytherea at Palmyra Atoll, and creation and emended description of Vibrio tetraodonis subsp. tetraodonis subsp. nov

Cited by 3 publications

References 65 publications

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

TheVibrioType III Secretion System 2 is not restricted to theVibrionaceaeand encodes differentially distributed repertoires of effector proteins

Characterization and genome analysis of Vibrio phage vB_VhaP_PG11, representing a new viral genus

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