Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilis

Gómez‐Gil, Bruno; Thompson, Fl; Thompson, Christopher; Swings, Jean

doi:10.1099/ijs.0.02430-0

Cited by 86 publications

(61 citation statements)

References 25 publications

(20 reference statements)

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“…This finding needs further experimental investigation to confirm that it relates to a specific pathogenic feature. Different species of bacteria that have been associated to other organisms were also characterized, including Vibrio rotiferianus associated with normal microbiota of rotifers (Gomez-Gil et al, 2003), and Microbacterium oxydans and Nocardiopsis alba associated with normal microbiota of different insects (Pidiyar et al, 2004;Rudolf et al, 2009;Patil et al, 2010). Other bacterial species involved in nosocomial infections were isolated, such as Stenotrophomonas rhizophila (normally associated with plants) (Wolf et al, 2002) and Stenotrophomonas maltophilia (Waters et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

et al. 2011

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Coral reefs are deteriorating at an alarming rate mainly as a consequence of the emergence of coral diseases. The white plague disease (WPD) is the most prevalent coral disease in the southwestern Caribbean, affecting dozens of coral species. However, the identification of a single causal agent has proved problematic. This suggests more complex etiological scenarios involving alterations in the dynamic interaction between environmental factors, the coral immune system and the symbiotic microbial communities. Here we compare the microbiome of healthy and WPD-affected corals from the two reef-building species Diploria strigosa and Siderastrea siderea collected at the Tayrona National Park in the Caribbean of Colombia. Microbiomes were analyzed by combining culture-dependent methods and pyrosequencing of 16S ribosomal DNA (rDNA) V5-V6 hypervariable regions. A total of 20 410 classifiable 16S rDNA sequences reads were obtained including all samples. No significant differences in operational taxonomic unit diversity were found between healthy and affected tissues; however, a significant increase of Alphaproteobacteria and a concomitant decrease in the Beta-and Gammaproteobacteria was observed in WPD-affected corals of both species. Significant shifts were also observed in the orders Rhizobiales, Caulobacteriales, Burkholderiales, Rhodobacterales, Aleteromonadales and Xanthomonadales, although they were not consistent between the two coral species. These shifts in the microbiome structure of WPD-affected corals suggest a loss of community-mediated growth control mechanisms on bacterial populations specific for each holobiont system. The ISME Journal (2012) 6, 502-512; doi:10.1038/ismej.2011.123; published online 29 September 2011Subject Category: microbial population and community ecology Keywords: bacterial community; white plague disease; coral diseases; pyrosequencing; Diploria strigosa; Siderastrea siderea IntroductionCoral reefs posses an immense biodiversity comparable only to that of the tropical rain forest (Mulhall, 2009). The structural, physiological and ecological bases of reefs are the scleractinian corals. They are in symbiotic relationship with a variety of bacteria and archaea as well as with microalgae (zooxanthellae), which are mainly responsible for the corals' high contribution to primary productivity of coral reefs . Modifications in the structure and relative density of symbiotic microbial communities might have a critical role in the coral adaptation to rapid environmental changes (Reshef et al., 2006). In the last three decades, local and global deterioration of environmental conditions have dramatically compromised the health of corals, which consequently affected the entire coral reef ecosystem. (Harvell et al., 1999(Harvell et al., , 2007Wilkinson, 1999;Green and Bruckner, 2000;Gardner et al., 2003;Pandolfi et al., 2003;Lesser et al., 2007). Recent reports indicate that 58-70% of coral reefs worldwide are threatened by human activities, while more than 30% of the biota associated with Caribb...

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Section: Discussionmentioning

confidence: 99%

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

et al. 2011

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“…Vibrio rotiferianus was assigned a species designation in 2003 on the basis of Ͻ70% DNA hybridization similarity value to its most closely related species, V. campbellii and V. harveyi, although they share 99% identity in 16S rRNA gene sequences (6). The microorganism was named after its isolation source, rotifer cultures, which serve as important nutrients for fish and crustaceans in aquaculture industries (6).…”

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confidence: 99%

“…The microorganism was named after its isolation source, rotifer cultures, which serve as important nutrients for fish and crustaceans in aquaculture industries (6). V. rotiferianus DAT722, a mild pathogen of mud crab larvae (2), was isolated from a mud crab larva aquaculture tank in the Northern Territory of Australia (3).…”

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confidence: 99%

Genome Sequence of Vibrio rotiferianus Strain DAT722

et al. 2011

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Vibrio rotiferianus is a marine pathogen capable of causing disease in various aquatic organisms. We announce the genome sequence of V. rotiferianus DAT722, which has a large chromosomal integron containing 116 gene cassettes and is a model organism for studying the role of this system in vibrio evolution.Vibrio rotiferianus was assigned a species designation in 2003 on the basis of Ͻ70% DNA hybridization similarity value to its most closely related species, V. campbellii and V. harveyi, although they share 99% identity in 16S rRNA gene sequences (6). The microorganism was named after its isolation source, rotifer cultures, which serve as important nutrients for fish and crustaceans in aquaculture industries (6).

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“…Although DNA-DNA hybridization values .60 % may be considered borderline, these values are quite common for different Aeromonas species (Nhung et al, 2007;Alperi et al, 2010). This also happens among species of the neighbouring genus Vibrio (Gó mez-Gil et al, 2003; Thompson et al, 2003).…”

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Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

Alperí

Martínez‐Murcia

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Two clinical Aeromonas strains (A2-50 T and A2-67 T ) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of Aeromonas caviae, A. trota and A. aquariorum. The rpoD phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being A. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50 T and 92.2 % (63 bp differences) to strain A2-67 T . A multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA-DNA reassociation results, revealed that these strains represent novel Aeromonas species, for which the names Aeromonas taiwanensis sp. nov. (type strain A2-50 T 5CECT 7403 T 5LMG 24683 T ) and Aeromonas sanarellii sp. nov. (type strain A2-67 T 5CECT 7402 T 5LMG 24682 T ) are proposed.The genus Aeromonas includes facultatively anaerobic, Gramnegative, non-spore-forming bacilli or coccobacilli that are generally motile, usually oxidase-and catalase-positive, able to reduce nitrate to nitrite and generally resistant to the vibriostatic agent O/129 (2,4-diamino-6,7-diisopropylpteridine) (Abbott et al., 2003;Martin-Carnahan & Joseph, 2005). The genus belongs to the family Aeromonadaceae, order Aeromonadales, class Gammaproteobacteria The taxonomy of this genus is complex because, despite each species apparently having specific phenotypic characteristics, biochemical identification is laborious and very imprecise, showing poor correlation with genotypic identification (Borrell et al., 1998;Soler et al., 2003;Figueras, 2005;Ormen et al., 2005). The 16S rRNA gene sequence similarity among species of the genus is very high, ranging from 96.7 to 100 % (Martínez-Murcia et al., 1992Saavedra et al., 2006). Furthermore, this gene presents microheterogeneities Martínez-Murcia et al., 2005;Morandi et al., 2005;Alperi et al., 2008) that reduce its usefulness for identification (Alperi et al., 2008); such was the case for Aeromonas culicicola Alperi et al., 2008), now considered a synonym of A. veronii (Huys et al., 2005). However, several housekeeping genes have proven to have Abbreviation: MLPA, multilocus phylogenetic analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoD, recA, dnaJ and gyrA gene sequences of strains A2-50 T and A2-67 T are respectively FJ230077 and FJ230076, FJ807272 and FJ807277, FJ472928 and FJ472929, FJ472930 and FJ47931, FJ807270 and FJ807279 and FJ807274 and FJ807276. An unrooted 16S rRNA gene sequence-based maximum-parsimony phylogenetic tree, an unrooted MLPA minimum-evolution tree, details of differences in the 16S rRNA gene sequences of the novel strains and DNA-DNA hybridization results are available as supplementary material ...

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Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilis

Cited by 86 publications

References 25 publications

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

Genome Sequence of Vibrio rotiferianus Strain DAT722

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

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