Vibrio-infecting bacteriophages and their potential to control biofilm

Cevallos-Urena, Ana; Kim, Jeong Yeon; Kim, Byoung Sik

doi:10.1007/s10068-023-01361-7

Cited by 4 publications

(1 citation statement)

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“…Bacteriophages have been proposed as antimicrobials against pathogenic bacteria in different fields, including aquaculture [26]. The literature shows examples of phage applications focusing on the control of pathogenic Vibrios, one the major problems in aquaculture [27][28][29]39,40]. Following this research trend, in this study, a lytic phage was isolated to control the GV09 strain of antibiotic-resistant Vibrios.…”

Section: Discussionmentioning

confidence: 99%

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

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Vibrio species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1’s lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only Vibrio alginolyticus GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio splendidus. Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.

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