Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters (Crassostrea gigas)

Roux, Frédérique Le; Goubet, Anne; Thompson, Fl; Faury, Nicole; Swings, Jean; Saulnier, Denis

doi:10.1099/ijs.0.63666-0

Cited by 72 publications

(47 citation statements)

References 17 publications

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“…gallaecicus (Beaz-Hidalgo et al, 2009), V. gigantis (Le Roux et al, 2005), V. hemicentroti (Kim et al, 2013), V. kanaloae (Thompson et al, 2003c), V. lentus (Macian et al, 2001), V.…”

Section: Vibrio Splendidus-relatedmentioning

confidence: 99%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

142

121

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Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.

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“…gallaecicus (Beaz-Hidalgo et al, 2009), V. gigantis (Le Roux et al, 2005), V. hemicentroti (Kim et al, 2013), V. kanaloae (Thompson et al, 2003c), V. lentus (Macian et al, 2001), V.…”

Section: Vibrio Splendidus-relatedmentioning

confidence: 99%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

142

121

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“…Le Roux et al (2005) described Vibrio gigantis on the basis of individual gene trees, comparing the four strains of the species with type strains of the Vibrio splendidus clade. This clade contains eight species that are related genomically and phylogenetically as closely as the core group.…”

Section: Concatenated Gene Sequencesmentioning

confidence: 99%

Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes

Pascual

Macián

Arahal

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

194

175

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Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes The central clade of the genus Vibrio, also called the Vibrio core group, comprises six species that are tightly related (DNA-DNA reassociation values are very close to 70 % for most species pairs). Identification of novel strains to the species level within this group is troublesome and results are quite often dependent on the methodology employed. Therefore, this group represents an excellent framework to test the robustness of multilocus sequence analysis (MLSA) not only for inferring phylogeny but also as an identification tool without the need for DNA-DNA hybridization assays. The genes selected, 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR, were amplified by direct PCR from 44 Vibrio core-group strains. Subsequent analysis allowed us to recognize toxR and rpoD as the most resolving individual genes and showed that concatenated sequences of rpoD, rctB and toxR were more useful than concatenated sequences of all seven genes. To validate our conclusions, MLSA similarities have been correlated with DNA-DNA relatedness values obtained in this study and values taken from the literature. Although the seven concatenated genes gave the best correlation, the concatenated sequences of rpoD, rctB and toxR have the practical advantage of showing a considerable gap between the maximal interspecies similarity and the minimal intraspecies similarity recorded, meaning that they can be used quite conveniently for species identification of vibrios. INTRODUCTIONMLSA (multilocus sequence analysis) is a recently developed technique derived from multilocus sequence typing that has been used successfully in epidemiology studies. The use of MLSA as an alternative method for species delineation in bacteriology is currently being evaluated, following a recommendation of the ad hoc committee for the re-evaluation of the species definition in bacteriology (Stackebrandt et al., 2002). In MLSA studies, several housekeeping genes (more than five) are analysed and relationships between taxa are established. One important advantage of this kind of approach is the availability of genomic sequences from any laboratory, avoiding the problems of lack of comparability that are associated with DNA-DNA reassociation data. However, the usefulness of MLSA for describing bacterial species has to be demonstrated for each of the taxa or group of species studied and for the selected housekeeping genes. Authors should prove that there is a sufficient degree of congruence between the alternative technique (i.e. MLSA) and DNA-DNA reassociation data. To date, the technique has been applied to discrete bacterial taxa, mainly genera of lactic acid bacteria (Naser et al. et al., 2007, 2008) and the Vibrionaceae (Goarant et al., 2006;Urbanczyk et al., 2007;Thompson et al., 2008;Rameshkumar et al., 2008).In the family Vibrionaceae, sequence similarities for the 16S rRNA gene are ¢97.6 % among members of the so-called Vib...

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“…Reference strains included the type strains of E. norvegicus, E. coralii, V. calviensis, G. hollisae and Vibrio cholerae (Table 1). Amplification and partial sequencing of genes coding for 16S rRNA, recA and rpoD were performed as described previously by Macián et al (2001b), Thompson et al (2005a) and Le Roux et al (2005), respectively. Multiple sequence alignments were obtained using CLUSTAL_X (Thompson et al, 1997).…”

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confidence: 99%