Vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (PAH)-degrading marine bacterium.

Abstract

Strain P-2P44 T was isolated from creosote-contaminated marine sediments by using a most-probable number procedure in which phenanthrene was the sole carbon and energy source. Growth experiments showed that P-2P44 T utilized several two-and three-ring polycyclic aromatic hydrocarbons (PAHs) as substrates, including naphthalene, 2-methylnaphthalene and phenanthrene. Additionally, gas-chromatography experiments showed that P-2P44 T degraded several other PAHs, though it was unable to use them as sole sources of carbon and energy. Phylogenetic analyses confirmed that strain P-2P44 T is a member of the genus Vibrio, most closely related to Vibrio splendidus. However, strain P-2P44 T shared only 98 3 % 16S rDNA identity and 35 % DNA-DNA reassociation with the type strain of V. splendidus. Strain P-2P44 T differed phenotypically from V. splendidus. Together, these differences indicated that strain P-2P44 T represents a novel species in the genus Vibrio, for which the name Vibrio cyclotrophicus sp. nov. is proposed ; the type strain is P-2P44 T (l ATCC 700982 T l PICC 106644 T ).

Marine Oil-Degrading Microorganisms and Biodegradation Process of Petroleum Hydrocarbon in Marine Environments: A Review

“…Compared with conventional physical and chemical methods, bioremediation technology is less expensive and highly effective for the marine ecological restoration, which has the following advantages [20,32,40,80].…”

“…1, which can be classified into three forms: subterminal oxidation, alkyl hydroperoxides, and degradation of cyclohexane [32]. Basically, alkane is catalyzed by some enzymes (e.g., oxidase, dehydroge, etc.)…”

“…Petroleum is one of the most important raw materials for the production of petroleum fuels (e.g., gasoline and kerosene) and chemical reagents products (e.g., pharmaceuticals, solvents, fertilizers, and plastics) [32]. Due to the increasing global petroleum demand, more and more activities such as exploitation, extraction, refining, and transportation of petroleum have been conducted.…”

Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium

“…Some PAHs are highly carcinogenic, genotoxic and cytotoxic to marine organisms and may be transferred to humans through seafood consumption (Menzie et al, 1992). Therefore, removal of PAHs from contaminated marine environments is of considerable importance.Several PAH-degrading strains have been isolated from PAH-contaminated marine sediments (Hedlund & Staley, 2001;Kwon et al, 2005;Sohn et al, 2004). In this study, we report the characterization of a PAH-degrading marine bacterium that was isolated from saline soil contaminated with crude oil in Xianhe, Shangdong Province, China.…”

“…The name Thalassospira xianhensis sp. nov. is proposed, with P-4 T (5CGMCC 1.6849 T 5JCM 14850 T ) as the type strain.Polycyclic aromatic hydrocarbons (PAHs) are hydrocarbons that consist of two or more fused aromatic rings (Habe & Omori, 2003;Hedlund & Staley, 2001). PAHs are released into the marine environment as a result of various anthropogenic activities such as marine seepage and accidental discharges during the transport and disposal of petroleum products and the use of fossil fuels (Sohn et al, 2004).…”