Vibrio cholerae pathogen from the freshwater-cultured whiteleg shrimp Penaeus vannamei and control with Bdellovibrio bacteriovorus

Cao, Haipeng; Jian, An; Zheng, Weidong; He, Shan

doi:10.1016/j.jip.2015.06.002

Cited by 58 publications

(37 citation statements)

References 26 publications

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“…Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Moraxella bovis (5), Aeromonas hydrophila in fish (6,7), Vibrio cholerae and Proteus penneri in shrimp (8,9), and Salmonella enterica serovar Enteritidis in the chicken gut (10). They have also been applied in the treatment of human disease pathogens, including Pseudomonas aeruginosa from the lungs of cystic fibrosis patients (11), Pseudomonas aeruginosa and Serratia marcescens associated with eye infections (12), and oral pathogens that cause periodontal diseases (13,14).…”

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confidence: 99%

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Richards

Fay

Uknalis

et al. 2016

Appl Environ Microbiol

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c Halobacteriovorax (formerly Bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. Four strains of Halobacteriovorax originally isolated in Vibrio parahaemolyticus O3:K6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. This technique was essential, since 0.45-m filtration alone was unable to remove infectious Vibrio minicells, as determined by scanning electron microscopy and cultural methods. Purified Halobacteriovorax strains were screened for predation against other V. parahaemolyticus strains and against Vibrio vulnificus, Vibrio alginolyticus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium DT104, all potential threats to seafood safety. They showed high host specificity and were predatory only against strains of V. parahaemolyticus. In addition, strains of Halobacteriovorax that were predatory for E. coli O157:H7 and S. Typhimurium DT104 were isolated from a tidal river at 5 ppt salinity. In a modified plaque assay agar, they killed their respective prey over a broad range of salinities (5 to 30 ppt). Plaques became smaller as the salinity levels rose, suggesting that the lower salinities were optimal for the predators' replication. These species also showed broader host specificity, infectious against each other's original hosts as well as against V. parahaemolyticus strains. In summary, this study characterized strains of Halobacteriovorax which may be considered for use in the development of broad-based biocontrol technologies to enhance the safety of commercially marketed shellfish and other foods. Bdellovibrio and like organisms (BALO) are Gram-negative, aerobic bacteria which are predatory toward other Gram-negative bacteria and consist of freshwater/terrestrial and marine forms. Bdellovibrio and like organisms are divided into four genera, Bdellovibrio, Bacteriolyticum, Peredibacter, and Bacteriovorax, which was recently renamed Halobacteriovorax (1). These organisms enter into a susceptible host bacterium and reside within the periplasmic space where they utilize the cytoplasmic nutrients of the host to support growth and replication. The replicative form, known as a bdelloplast, elongates within the host and septates into progeny cells as the host is lysed, releasing cells capable of attacking more prey. Halobacteriovorax organisms are the marine forms, which are small, polar flagellated, highly motile, intracellular predators in the class Deltaproteobacteria family Halobacteriovoraceae (1).Bdellovibrio and like organisms, particularly the nonhalophilic Bdellovibrio bacteriovorus, have been proposed for use in reducing the levels of phytopathogens associated with bacterial blights in soybeans and rice (2, 3) and reducing the levels of Salmonella enterica serovar Typhimurium on freshwater fish fillets (4). Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Mora...

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confidence: 99%

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Richards

Fay

Uknalis

et al. 2016

Appl Environ Microbiol

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“…Species matching of disappeared bands (Band 1,2,3,4,5,and 6) invade, grow and reproduce (Martin 2002). These bacteria play important roles in reducing microbial density and altering microbial communities through predation (Cao et al 2015). They prey on other Gram-negative species and hence act as a population balancer (Iebba et al 2013) or as an alternative to antibiotics to remove harmful and pathogenic bacteria or a probiotic to help curb and control bacterial populations within the intestinal tract (Dwidar et al 2012) or even as a biocontrol/probiotic agent to curb snakehead fish-pathogenic A. veronii (Cao et al 2013) and to enhance growth and survival of P. monodon in its rearing water (Li et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Vibriosis is one of the major threats in marine or freshwater-farmed fish and shellfish aquaculture worldwide (Mahmud et al 2010;Alves et al 2011;Zhou and Cai 2013;Cao et al 2015). Numerous Vibrio species have been reported as causal agents of diseases and the decrease of yields in various fish species, including turbot (Cerdà-Cuéllar and Blanch 2004; Thomson et al 2005;Toranzo et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, sequencing results showed that two of the disappeared bands belonged to Vibrio (Table 3). For this, our finding was supported by Cao et al (2015), who demonstrated that Bdellovibrio bacteriovorus exhibited significant bacteriolytic effects on Vibrio cholera, and displayed a positive protective efficacy against experimental V. cholerae in shrimp Penaeus vannamei. These data support the notion that BDW03 can function as a bacterial controller.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Bdellovibrio and Like Organisms on Survival and Growth Performance of Juvenile Turbot, Scophthalmus maximus

Guo

Yan

Cai

2016

J World Aquaculture Soc

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In this study, the impact of Bdellovibrio and like organisms (BALOs) on juvenile turbot rearing was investigated in a static water rearing system with weekly partial change of water. A 60‐d‐long rearing experiment showed that, compared with control, survival rate, percent weight gain, percent length gain, and average daily growth were 92 ± 2.8%, 387.1 ± 4.6%, 78.6 ± 1.5%, and 1.87 ± 0.79 g/d, respectively, significantly higher than those in the control, viz., 81 ± 3.2%, 248.2 ± 5.3%, 56.7 ± 2.1%, and 1.16 ± 0.68 g/d, respectively. Water quality results demonstrated that no significant difference (P > 0.05) was observed between test and control with the addition of BDW03. Compared with the control, total culturable bacteria and total vibrio numbers in rearing water and in intestines were significantly (P < 0.05) reduced by 0.8–1.4 log CFU/mL/ log CFU/g, with the addition of BDW03. Furthermore, denaturing gradient gel electrophoresis profiles in rearing waters collected at a 1.5‐d interval over a week revealed that BDW03 simplified bacterial community structures with time, and some vibrios disappeared with the addition of BDW03. To the best of our knowledge, this is the first report that BALOs can promote growth and survival of juvenile turbots and be beneficial to coldwater fish aquaculture at the production level.

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“…For example, a single or defined consortium of probiotics is generally applied over the entire shrimp culture cycle even though the potential pathogens vary by growth stages (Zheng et al 2016). As a consequence, the probiotic effects sustain only for a short time and even are invalid (Ninawe and Selvin 2009;Wang et al 2008), though case studies have extensively validated the successes of probiotics in shrimp aquaculture (Cao et al 2015;Li et al 2007;Solanki et al 2015). However, a survey of farmers showed that they have limited success with probiotics application.…”

Section: Establishment Of Shrimp Age-dependent Treatment Strategiesmentioning

confidence: 99%

Advances, challenges, and directions in shrimp disease control: the guidelines from an ecological perspective

Xiong

Dai

2016

Appl Microbiol Biotechnol

105

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High-density aquaculture has led to increasing occurrences of diseases in shrimp. Thus, it is imperative to establish effective and quantitative strategies for preventing and predicting these diseases. Water quality indices and investigations of specific pathogen abundance provide only a qualitative evaluation of the risk of shrimp disease and can be inaccurate. To address these shortcomings, we introduced intestinal indicative assemblages as independent variables with which to quantitatively predict incidences of shrimp disease. Given the ignorance regarding the niches differences in the shrimp intestine throughout its developmental stages, the use of probiotics in aquaculture has had limited success. Therefore, we propose the exploration of effective probiotic bacteria from shrimp intestinal flora and the establishment of therapeutic strategies dependent on shrimp age. Following ecological selection principles, we hypothesize that the larval stage provides the best opportunity to establish a desired gut microbiota through preemptive colonization of the treated rearing water with known probiotics. To employ this strategy, however, substantial barriers must be overcome.

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Vibrio cholerae pathogen from the freshwater-cultured whiteleg shrimp Penaeus vannamei and control with Bdellovibrio bacteriovorus

Cited by 58 publications

References 26 publications

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Effects of Bdellovibrio and Like Organisms on Survival and Growth Performance of Juvenile Turbot, Scophthalmus maximus

Advances, challenges, and directions in shrimp disease control: the guidelines from an ecological perspective

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