Vibrational spectrum of the K-590 intermediate in the bacteriorhodopsin photocycle at room temperature: picosecond time-resolved resonance coherent anti-Raman spectroscopy

Ujj, Laszlo; Jäger, Frank; Popp, Andreas; Atkinson, Gordon

doi:10.1016/s0301-0104(96)00201-7

Cited by 17 publications

(86 citation statements)

References 41 publications

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“…“Pure” spectra of individual species that are crucial for kinetic analyses of complex reactions can only be accessed by subtraction of residual contributions from other states. ,, This procedure was substantially improved by introducing band fitting routines . In CARS and FSRS experiments, the scheme to extract RR spectra from the raw data is even more complicated because of other competing nonlinear processes. ,,, …”

Section: Photoinduced Processesmentioning

confidence: 93%

“…The high velocity and the resulting short residence time of the sample in a tightly focused laser spot (Δ t ≈ 2 μs ) is advantageous for experiments that involve short laser pulses and aim at high time resolution. Due to the high flow speed, the “fresh sample” condition is preserved even at high pulse repetition rates up to the MHz regime. , Thus, the liquid jet system facilitated early RR studies with ps time resolution, − as well as ps TR CARS experiments. − …”

Section: Photoinduced Processesmentioning

confidence: 99%

“…In this way, the short-lived K590 and J625 intermediates were probed . Later, these early ground state intermediates were also investigated with ps TR CARS spectroscopy, where the conventional ps probe–pulse sequence was replaced by an elaborate probe scheme consisting of two nonlinear mixed laser pulses. ,, …”

Section: Photoinduced Processesmentioning

confidence: 99%

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Probing Structure and Reaction Dynamics of Proteins Using Time-Resolved Resonance Raman Spectroscopy

Buhrke

Hildebrandt

2019

Chem. Rev.

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The mechanistic understanding of protein functions requires insight into the structural and reaction dynamics. To elucidate these processes, a variety of experimental approaches are employed. Among them, time-resolved (TR) resonance Raman (RR) is a particularly versatile tool to probe processes of proteins harboring cofactors with electronic transitions in the visible range, such as retinal or heme proteins. TR RR spectroscopy offers the advantage of simultaneously providing molecular structure and kinetic information. The various TR RR spectroscopic methods can cover a wide dynamic range down to the femtosecond time regime and have been employed in monitoring photoinduced reaction cascades, ligand binding and dissociation, electron transfer, enzymatic reactions, and protein un-and refolding. In this account, we review the achievements of TR RR spectroscopy of nearly 50 years of research in this field, which also illustrates how the role of TR RR spectroscopy in molecular life science has changed from the beginning until now. We outline the various methodological approaches and developments and point out current limitations and potential perspectives.

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Section: Photoinduced Processesmentioning

confidence: 93%

Section: Photoinduced Processesmentioning

confidence: 99%

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Probing Structure and Reaction Dynamics of Proteins Using Time-Resolved Resonance Raman Spectroscopy

Buhrke

Hildebrandt

2019

Chem. Rev.

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“…Many aspects of the PTR/CARS methodology have been derived from its application to intermediates in the BR photocycle [5]. The BR R" photocycle is especially attractive experimentally since it is reversible (within can 5 ms) reaction sequence that ensures a longlived protein sample without significant optical deterioration.…”

mentioning

confidence: 99%

“…and BR-548) [6], and the separation of CARS spectrum assignable to a transient intermediate (K-590) from PTR/CARS data recorded from a mixture containing static and transient species [5] are all critical advances made initially via studies of the BR photocycle.…”

mentioning

confidence: 99%

Reactive Intermediates in the Room Temperature Rhodopsin Photo-Reactions: Picosecond Time-Resolved Cars

1999

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Coherent Anti‐Stokes R aman Spectroscopy

Ujj

Atkinson

2001

Handbook of Vibrational Spectroscopy

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Coherent anti‐Stokes Raman spectroscopy (CARS) has been shown to be one of the most powerful experimental methodologies for obtaining vibrational information from both stable and transient molecular species. The general theoretical and experimental principles associated with CARS, together with specific examples of its applications to important biological systems, are presented. A concise theoretical formalism of CARS, and its electronically resonant variant, is presented with an emphasis on points relevant to the interpretation of experimental spectra. Particular attention is given to deriving vibrational signatures from the Raman resonances observed in CARS data. The instrumental components required for obtaining typical CARS data are illustrated in a brief description of an experimental system used to record CARS spectra from biophysical molecules (photoactive protein reactions). Specific examples of CARS data are taken from measurements of stable and transient intermediates comprising the photoreactions of the bacteriorhodopsin (BR) and rhodopsin (Rh) proteins. Finally, an overview of developments to be anticipated in this field is presented.

show abstract

Vibrational spectrum of the K-590 intermediate in the bacteriorhodopsin photocycle at room temperature: picosecond time-resolved resonance coherent anti-Raman spectroscopy

Cited by 17 publications

References 41 publications

Probing Structure and Reaction Dynamics of Proteins Using Time-Resolved Resonance Raman Spectroscopy

Probing Structure and Reaction Dynamics of Proteins Using Time-Resolved Resonance Raman Spectroscopy

Reactive Intermediates in the Room Temperature Rhodopsin Photo-Reactions: Picosecond Time-Resolved Cars

Coherent Anti‐Stokes R aman Spectroscopy

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