Vibrational spectroscopies for biochemical investigation of X-ray exposure effects on SH-SY5Y human neuroblastoma cells

Ricciardi, Valerio; Lasalvia, Maria; Perna, G.; Portaccio, Marianna; Delfino, Ines; Lepore, Maria; Capozzi, V.; Manti, Lorenzo

doi:10.1007/s00411-023-01035-2

Cited by 2 publications

(2 citation statements)

References 84 publications

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“…The main peaks contributing to data separation in PC-2 are related to the AI, the carbonyl ester, the -helix of AII and the CH 2 bending modes; these bands were also relevant for astrocytes and may indicate similar biochemical processes. Additionally, two peaks arise at 1529 cm -1 and 1505 cm -1 , assigned to -sheets of AII 37 and to CH in-plane bending 27 , respectively; these are especially relevant for the highest dose. Regarding PC-1, the -sheet of AI is highly contributing to data separation, and may indicate secondary structure protein modifications due to oxidative stress or cell death processes, particularly for the highest dose of pMBRT.…”

Section: Resultsmentioning

confidence: 99%

Investigating the biochemical response of proton minibeam radiation therapy by means of synchrotron-based infrared microspectroscopy

González-Vegas,

Yousef,

Seksek

et al. 2024

Sci Rep

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The biology underlying proton minibeam radiation therapy (pMBRT) is not fully understood. Here we aim to elucidate the biological effects of pMBRT using Fourier Transform Infrared Microspectroscopy (FTIRM). In vitro (CTX-TNA2 astrocytes and F98 glioma rat cell lines) and in vivo (healthy and F98-bearing Fischer rats) irradiations were conducted, with conventional proton radiotherapy and pMBRT. FTIRM measurements were performed at ALBA Synchrotron, and multivariate data analysis methods were employed to assess spectral differences between irradiation configurations and doses. For astrocytes, the spectral regions related to proteins and nucleic acids were highly affected by conventional irradiations and the high-dose regions of pMBRT, suggesting important modifications on these biomolecules. For glioma, pMBRT had a great effect on the nucleic acids and carbohydrates. In animals, conventional radiotherapy had a remarkable impact on the proteins and nucleic acids of healthy rats; analysis of tumour regions in glioma-bearing rats suggested major nucleic acid modifications due to pMBRT.

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Section: Resultsmentioning

confidence: 99%

Investigating the biochemical response of proton minibeam radiation therapy by means of synchrotron-based infrared microspectroscopy

González-Vegas,

Yousef,

Seksek

et al. 2024

Sci Rep

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“…The evaluation of ratios between the areas or the intensities of selected bands of infrared spectra is particularly useful for estimating changes induced in a certain class of samples by the interaction with physicochemical agents, as reported in Refs. [17,63,72]. In fact, many researchers used some of the presented ratios for evaluating the effects of interaction between cells and tissues with external agents.…”

Section: Ratiometric Analysis Of Atr-ftir Lipid Spectramentioning

confidence: 99%

Probing Biochemical Differences in Lipid Components of Human Cells by Means of ATR-FTIR Spectroscopy

Portaccio,

Faramarzi,

Lepore

2023

Biophysica

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Infrared spectroscopy has emerged as a promising technique for studying the composition of biological samples like lipids that play important roles in cellular functions and are involved in various diseases. For this reason, lipids are a target of interest in many biomedical studies. The objective of the present study is to utilize Fourier-Transform Infrared (FT-IR) spectroscopy to examine the main lipid components of human cells (phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, C18 ceramide, sphingosine-1-phosphate, ceramide-1-phosphate, sphingomyelin, cholesterol, and triolein). FT-IR analysis on the previously mentioned lipid samples was performed in Attenuated Total Reflection (ATR) mode. The obtained spectra clearly evidence the contributions of the different functional groups that are present in the examined samples. Detailed assignments of spectral features were carried out in agreement with the literature. Similarities and differences among the different types of commercial lipid samples are evidenced and discussed, with particular attention to phospholipid and sphingolipid components. A quantitative analysis of phosphatidylinositol and sphingomyelin spectra using a ratiometric approach is reported. Moreover, a reconstruction procedure of FT-IR spectra of complex lipids useful for chemometrics applications is described. These representative examples of the potential use of the results of the present study can certainly contribute to a larger use of FT-IR spectroscopy in lipidomics.

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Vibrational spectroscopies for biochemical investigation of X-ray exposure effects on SH-SY5Y human neuroblastoma cells

Cited by 2 publications

References 84 publications

Investigating the biochemical response of proton minibeam radiation therapy by means of synchrotron-based infrared microspectroscopy

Investigating the biochemical response of proton minibeam radiation therapy by means of synchrotron-based infrared microspectroscopy

Probing Biochemical Differences in Lipid Components of Human Cells by Means of ATR-FTIR Spectroscopy

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