Vibrational spectroscopic studies of lipid domains in biomembranes and model systems

Mendelsohn, Richard; Moore, David J.

doi:10.1016/s0009-3084(98)00085-1

Cited by 178 publications

(191 citation statements)

References 61 publications

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“…1). The assignment of most of the vibrational resonances in the CHstretch region is known [28,29]. Most prominent is the peak at 2847 cm -1 , which is due to the symmetric methylene stretch vibration.…”

Section: Resultsmentioning

confidence: 99%

Direct extraction of Raman line-shapes from congested CARS spectra

Vartiainen¹,

Rinia²,

Müller³

et al. 2006

Opt. Express

227

212

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Direct extraction of Raman line-shapes from congested CARS spectra.Vartiainen, E.M.; Rinia, H.A.; Müller, M.; Bonn, M. Published in: Optics Express DOI:10.1364/OE.14.003622 Link to publication Citation for published version (APA):Vartiainen, E. M., Rinia, H. A., Müller, M., & Bonn, M. (2006). Direct extraction of Raman line-shapes from congested CARS spectra. Optics Express, 14(8), 3622-3630. DOI: 10.1364/OE.14.003622 General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract:We show that Raman line-shapes can be extracted directly from congested coherent anti-Stokes Raman scattering (CARS) spectra, by using a numerical method to retrieve the phase-information hidden in measured CARS spectra. The proposed method utilizes the maximum entropy (ME) model to fit the CARS spectra and to further extract the imaginary part of the Raman susceptibility providing the Raman line-shape similar to the spontaneous Raman scattering spectrum. It circumvents the challenges arising with experimentally determining the real and imaginary parts of the susceptibility independently. Another important advantage of this method is that no a priori information regarding the vibrational resonances is required in the analysis. This permits, for the first time, the quantitative analysis of CARS spectra and microscopy images without any knowledge of e.g. sample composition or Raman response. ©2006 Optical Society of AmericaOCIS codes: (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering; (000.3860) General, mathematical methods in physics.References and links

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Section: Resultsmentioning

confidence: 99%

Direct extraction of Raman line-shapes from congested CARS spectra

Vartiainen¹,

Rinia²,

Müller³

et al. 2006

Opt. Express

227

212

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“…The wavenumbers of the symmetric (ν s CH 2 ) and antisymmetric (ν as CH 2 ) methylene stretching vibrational bands provide information about the conformational order of the alkyl chain. 38,39 The temperature dependency of the wavenumbers of both methylene stretching vibrational bands of PC-C17pPhC17-PC and PC-C17pPhC17-OH is depicted in Figure 3, and in the SI in Figures S5, S6. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 structures.…”

Section: Dsc and Ftirmentioning

confidence: 99%

The Headgroup (A)Symmetry Strongly Determines the Aggregation Behavior of Single-Chain Phenylene-Modified Bolalipids and Their Miscibility with Classical Phospholipids

et al. 2014

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In the present work, we describe the synthesis of two single-chain phenylene-modified bolalipids, namely PC-C17pPhC17-PC and PC-C17pPhC17-OH, with either symmetrical (phosphocholine) or asymmetrical (phosphocholine and hydroxyl) headgroups using a Sonogashira cross coupling reaction as key-step. The temperature-dependent aggregation behavior of both bolalipids in aqueous suspension was studied using transmission electron microscopy (TEM), differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, small angle neutron scattering (SANS), and X-ray scattering. We show that different headgroup symmetries lead to a change in the aggregation behavior: Whereas PC-C17pPhC17-PC forms nanofibers with a diameter of 5.7 nm that transform into small ellipsoidal micelles at 23 °C, the PC-C17pPhC17-OH self-assembles into lamellae with bolalipid molecules in an antiparallel orientation up to high temperatures. Furthermore, the mixing behavior of both bolalipids with bilayer forming phospholipids (DPPC and DSPC) was studied by means of DSC and TEM. The aim was to stabilize bilayer membranes formed of phospholipids in order to improve these mixed lipid vesicles for drug delivery purposes. We show that the symmetrical PC-C17pPhC17-PC is miscible with DPPC and DSPC, however, closed lipid vesicles are not observed and elongated micelles and bilayer fragments are found instead. In contrast, the asymmetrical PC-C17pPhC17-OH shows no miscibility with phospholipids at all.

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“…In binary mixtures, the acyl chain order of individual lipids can be analyzed independently by using an acyl chain deuterated lipid as one of the two components (28). That is, the acyl chain order of the deuterated component can be studied based upon the antisymmetric CD 2 stretching vibration band [ a (CD 2 )], whereas the symmetric CH 2 stretching band [ s (CH 2 )] is used to monitor the nondeuterated lipid.…”

Section: Infrared Spectroscopymentioning

confidence: 99%

pH-dependent domain formation in phosphatidylinositol polyphosphate/phosphatidylcholine mixed vesicles

Redfern

Gericke

2005

Journal of Lipid Research

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Phosphatidylinositol polyphosphates (PI-PPs) have been shown to mediate a large variety of physiological processes by attracting proteins to specific cellular sites. Such site-specific signaling requires local accumulation of PI-PPs, and in light of the rich headgroup functionality, it is conceivable that hydrogen bond formation between adjacent headgroups is a contributing factor to the formation of PI-PP-enriched domains. To explore the significance of hydrogen bond formation for the mutual interaction of PI-PPs, this study aims to characterize the pH-dependent phase behavior of phosphatidylcholine/phosphatidylinositol bisphosphate and trisphosphate mixed vesicles by differential scanning calorimetry, infrared transmission spectroscopy, and fluorescence resonance energy transfer measurements. For pH values Ͼ 7-7.5, the experiments yielded results consistent with dipalmitoylphosphatidylcholine/dipalmitoylphosphatidylinositol polyphosphate gel phase demixing, whereas for moderately acidic conditions, an enhanced mixing was observed. Similarly, this pH-dependent formation of PI-PPenriched domains was also found for the physiologically important fluid phase. The stability of PI-PP-enriched domains and to some extent the pH dependence of the domain formation was governed by the number as well as the position of the phosphomonoester groups at the inositol ring. ( Fig. 1 ) have been shown to control membrane trafficking events by recruiting proteins to specific cellular sites (1, 2). The temporal control of phosphoinositide-mediated signaling events is facilitated by a broad range of kinases and phosphatases (1, 3), whereas the signaling specificity is rooted in selective protein binding as well as a high degree of phosphoinositide compartmentalization. This compartmentalization is not only evident in the accumulation of distinct phosphoinositide derivatives in particular cell entities [e.g., enrichment of phosphatidylinositol-3-phosphate in early endosomes and phosphatidylinositol-4-phosphate in the Golgi (2, 4-6)] but manifests itself also in the formation of phosphoinositide-enriched domains. Several studies have reported the raft-dependent (7-13) and raft-independent (14-17) accumulation of phosphoinositides in vivo; however, the mechanisms that lead to such phosphoinositide segregation are elusive. For example, the recruitment of certain phosphoinositides to raft domains raises the question of what kind of molecular interactions and physiological conditions aid such targeting, which is generally unfavorable for lipids with unsaturated acyl chains (the typical chain composition of phosphoinositides is stearoyl/arachidonoyl). Simple lipid partitioning models cannot explain phosphoinositide enrichment in rafts, because measurements on monolayer systems failed to show any phosphatidylinositol-4,5-bisphosphate (PI-4,5-P 2 ) accumulation in cholesterol-enriched domains (although such studies have some limitations when compared with bilayer systems) (18).

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Vibrational spectroscopic studies of lipid domains in biomembranes and model systems

Cited by 178 publications

References 61 publications

Direct extraction of Raman line-shapes from congested CARS spectra

Direct extraction of Raman line-shapes from congested CARS spectra

The Headgroup (A)Symmetry Strongly Determines the Aggregation Behavior of Single-Chain Phenylene-Modified Bolalipids and Their Miscibility with Classical Phospholipids

pH-dependent domain formation in phosphatidylinositol polyphosphate/phosphatidylcholine mixed vesicles

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