Vibrational Microspectroscopy for Analysis of Atherosclerotic Arteries

Marzec, Katarzyna M.; Wróbel, Tomasz P.; Fedorowicz, Andrzej; Mateuszuk, Łukasz; Maślak, Edyta; Jasztal, Agnieszka; Chłopicki, Stefan

doi:10.1007/978-94-007-7832-0_17

Cited by 3 publications

(2 citation statements)

References 110 publications

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“…This chemical contrast can be used to enable more precise models of pathology classification for many diseases, e.g. cancers, atherosclerosis or diabetes 7 – 12 . In recent years there has been a tremendous progress in instrumentation and image formation theory, leading to new modalities among which High Definition (HD) pushed the projected pixel size to diffraction limit sampling 13 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of spectral and spatial denoising techniques in the context of High Definition FT-IR imaging hyperspectral data

Koziol

Raczkowska

Skibińska

et al. 2018

Sci Rep

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The recent emergence of High Definition (HD) FT-IR and Quantum Cascade Laser (QCL) Microscopes elevated the IR imaging field very close to clinical timescales. However, the speed of acquisition and data quality are still the critical factors in reaching the clinic. Denoising offers aide in both aspects if performed properly. However, there is a lack of a direct comparison of the efficiency of denoising techniques in IR imaging in general. To achieve such comparison within a rigorous framework and obtaining the critical information about signal loss, a simulated dataset strongly bound by experimental parameters was created. Using experimental structural and spectral information and experimental noise levels data as an input for the simulation, a direct comparison of spatial (Fourier transform, Mean Filter, Weighted Mean Filter, Gauss Filter, Median Filter, spatial Wavelets and Deep Neural Networks) and spectral (Savitzky-Golay, Fourier transform, Principal Component Analysis, Minimum Noise Fraction and spectral Wavelets) denoising schemes was enabled. All of these techniques were compared on the simulated dataset, taking into account SNR gain, signal distortion and sensitivity to tuning parameters as comparison metrics. Later, the best techniques were applied to experimental data for validation. The results presented here clearly show the benefit of using hyperspectral denoising schemes such as PCA and MNF which outperform other methods.

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Section: Introductionmentioning

confidence: 99%

Comparison of spectral and spatial denoising techniques in the context of High Definition FT-IR imaging hyperspectral data

Koziol

Raczkowska

Skibińska

et al. 2018

Sci Rep

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show abstract

“…Vibrational spectroscopy is a potential method for the investigation of biological tissues. FTIR microspectroscopy is a non-destructive analytical vibrational spectroscopy technique used for the identification of chemical and structural information within the tissue specimens [33][34][35]. FTIR microspectroscopy visually demonstrates the spatially resolved specific chemical functionalities in the form of heat maps.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Atherosclerotic Plaque Tissue

Paritala

2020

Adv. Mater. Lett.

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Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe

Dochow

Fatakdawala

Phipps

et al. 2016

Journal of Biophotonics

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Fluorescence lifetime imaging (FLIm) and Raman spectroscopy are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches are complementary and may also be used in combination with OCT / IVUS to add chemical specificity to these morphologic intravascular imaging modalities. In this contribution, both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe. A previously trained SVM model was used to interpret the fluorescence lifetime data; integrated band intensities displayed in RGB false color images were used to interpret the Raman data. Both modalities demonstrate unique strengths and weaknesses and these will be discussed in comparison to histologic analyses from the two coronary arteries imaged.

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Vibrational Microspectroscopy for Analysis of Atherosclerotic Arteries

Cited by 3 publications

References 110 publications

Comparison of spectral and spatial denoising techniques in the context of High Definition FT-IR imaging hyperspectral data

Comparison of spectral and spatial denoising techniques in the context of High Definition FT-IR imaging hyperspectral data

Characterization of the Atherosclerotic Plaque Tissue

Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe

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