Vibration for enhancement of electrochemical analysis of biomolecules in a droplet on the rough surface of a disposable working electrode

Wang, Yahong; Lin, Xingchen; Cheng, Ye; Wang, Hua; Liu, Wu; Zhuge, Xiangkai; Huo, Xiao-Lei; Bao, Ning

doi:10.1016/j.aca.2023.341158

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…Electrochemical technology is an effective and powerful tool for real-time monitoring of biological information due to its high sensitivity, fast response, experimental simplicity, and low cost. , Integration of electrochemical biosensors with 3D cell culture can be crucial to assess the biochemical responses of cell biology. For example, H 2 O 2 is one of the major reactive oxygen species (ROS) in cellular metabolism and plays an important biological function as a signal molecule in signal transduction, inflammation, carcinogenesis, and neurodegenerative injury. − Real-time monitoring their release from 3D cultured cells can help to gain a deeper understanding of the pathological processes and molecular mechanisms. ,, Some attempts have been made for real-time sensing of biomolecules released from cells in the 3D culture. − However, these research studies used planar electrodes for sensing and only the released biomolecules spread to the surface of the electrode could be detected. − Meanwhile, long diffusion distances from cells in 3D culture models to planar electrodes can cause significant recombination or degradation of the unstable biomolecules such as H 2 O 2 , leading to an unreliable result.…”

Section: Introductionmentioning

confidence: 99%

Stretchable Sponge-Based Electrochemical Biosensor for Real-Time Sensing of Cells in Three-Dimensional Culture

Zhu,

Lu,

Peng

et al. 2023

Anal. Chem.

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For the study of cell biology, real-time information on cell physiological processes will be more accurate and closer to the in vivo condition in a three-dimensional (3D) culture system. Although most reported 3D cell culture scaffolds can better mimic the in vivo dynamic microenvironment, the real-time analysis technique is deficient or lacking. Herein, a stretchable and conductive 3D scaffold is developed to construct an electrochemical biosensor for real-time monitoring of cell release in 3D culture under stimulation of drug stimulant and mechanical force. In our design, the polyurethane sponge (PU) dipped with conductive carbon ink (CC/PU) was used as a conductive scaffold, and gold nanoparticles (nano-Au) were electrodeposited on the CC/PU (nano-Au CC/PU) to improve the electrochemical sensing performance. The prepared nano-Au CC/PU scaffold exhibits a good electrocatalytic ability to H 2 O 2 with a linear range from 20 nM to 43 μM. Due to the great biocompatibility, HeLa cells can be cultured directly on the nano-Au CC/PU and the in situ and real-time tracking of H 2 O 2 secretion from cells was achieved. The results demonstrate that both the drug stimulant and mechanical force can rapidly activate the release of reactive oxygen species. This study indicates that the stretchable 3D sensing scaffold has good potential for cell biology research in an in vivo-like microenvironment and can be extensively used in the fields of tissue engineering, drug screening, and pathological research.

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