Viaminate ameliorates Propionibacterium acnes-induced acne via inhibition of the TLR2/NF-κB and MAPK pathways in rats

Cao, Junjie; Xu, Meifeng; Zhu, Longfei; Xiao, Shengxiang

doi:10.1007/s00210-022-02379-0

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Studies have shown that C. acnes activates TLRs mainly by promoting the signal transduction of the NF-κB and MAPKs pathways to activate the expression of downstream in ammatory factors, chemokines, and MMPs genes. [33,34] We observed the nuclear displacement of YTCY-EPs on NF-κB and AP-1 by immuno uorescence staining, and detected the content of related conduction factors by Western blot and qRT-PCR. The results of these experiments show that, in the role of cells, YTCY-EPs can regulate the NF-κB and MAPKs pathways by regulating TLR2/TRAF6 to reduce the production of in ammatory factors, chemokines, and MMPs, thereby reducing or avoiding the development of acne in ammation problems.…”

Section: Discussionmentioning

confidence: 99%

Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways

Zhang,

Jiang,

Zhao

et al. 2023

Probiotics & Antimicro. Prot.

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As a probiotic, Weizmannia coagulans (W. coagulans) is often used in food and medicine to regulate intestinal ora and resist speci c in ammation. In this study, the anti-acne e cacy and mechanism of YTCY extracellular proteins (YTCY-EPs) from a strain of W. coagulans are analyzed. The main components of YTCY-EPs, extracted and separated from the fermentation broth, are peptides ranging from 1.51-11.44 kDa, accounting for about 80%. Among the peptides identi ed by LC/MS-MS, YTCY A-F possess the properties of antimicrobial peptides, while YTCY 1-4 possess antioxidative properties. These peptides have a strong effect on Cutibacterium acnes (C. acnes) and signi cantly inhibit Staphylococcus aureus. The adhesion of YTCY-EPs has a 50% inhibition rate. It is found that YTCY-EPs possess strong antioxidant and anti-in ammatory properties, and can reduce the downstream TLR2/NF-κB and MAPKs/AP-1 pathways by regulating the nuclear translocation of NF-κB and AP-1 in vitro. The transcriptional expression of in ammatory cytokines, in ammatory chemokines, and matrix metalloproteinase genes is also regulated, thereby slowing the recruitment of in ammatory cells and the development of in ammation, and increasing keratinocyte mobility. YTCY-EPs can also effectively solve such problems as erythema, papules, cysts, skin lesions, hyperkeratinization, and desquamation caused by C. acnes in rabbit ears. Additionally, the treatment effectively improves the condition of wounds and in ammation. The results of this study prove that YTCY-EPs can be used as a potential anti-acne raw material in cosmetics.

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Section: Discussionmentioning

confidence: 99%

Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways

Zhang,

Jiang,

Zhao

et al. 2023

Probiotics & Antimicro. Prot.

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Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6- Mediated NF-κB and MAPKs Signaling Pathways

Jiang

Zhao

et al. 2023

Preprint

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As a probiotic, Weizmannia coagulans (W. coagulans) is often used in food and medicine to regulate intestinal flora and resist specific inflammation. In this study, the anti-acne efficacy and mechanism of YTCY extracellular proteins (YTCY-EPs) from a strain of W. coagulans are analyzed. The main components of YTCY-EPs, extracted and separated from the fermentation broth, are peptides ranging from 1.51–11.44 kDa, accounting for about 80%. Among the peptides identified by LC/MS-MS, YTCY A-F possess the properties of antimicrobial peptides, while YTCY 1–4 possess antioxidative properties. These peptides have a strong effect on Cutibacterium acnes (C. acnes) and significantly inhibit Staphylococcus aureus. The adhesion of YTCY-EPs has a 50% inhibition rate. It is found that YTCY-EPs possess strong antioxidant and anti-inflammatory properties, and can reduce the downstream TLR2/NF-κB and MAPKs/AP-1 pathways by regulating the nuclear translocation of NF-κB and AP-1 in vitro. The transcriptional expression of inflammatory cytokines, inflammatory chemokines, and matrix metalloproteinase genes is also regulated, thereby slowing the recruitment of inflammatory cells and the development of inflammation, and increasing keratinocyte mobility. YTCY-EPs can also effectively solve such problems as erythema, papules, cysts, skin lesions, hyperkeratinization, and desquamation caused by C. acnes in rabbit ears. Additionally, the treatment effectively improves the condition of wounds and inflammation. The results of this study prove that YTCY-EPs can be used as a potential anti-acne raw material in cosmetics.

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Viaminate Inhibits Propionibacterium Acnes-induced Abnormal Proliferation and Keratinization of HaCat Cells by Regulating the S100A8/S100A9- MAPK Cascade

Cao,

Xu,

Zhu

et al. 2023

CDT

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Background: Viaminate, a vitamin A acid drug developed in China, has been clinically used in acne treatment to regulate epithelial cell differentiation and proliferation, inhibit keratinization, reduce sebum secretion, and control immunological and anti-inflammatory actions; however, the exact method by which it works is unknown. Methods: In the present study, acne was induced in the ears of rats using Propionibacterium acnes combined with sebum application Results: After 30 days of treatment with viaminate, the symptoms of epidermal thickening and keratin overproduction in the ears of rats were significantly improved. Transcriptomic analysis of rat skin tissues suggested that viaminate significantly regulated the biological pathways of cellular keratinization. Gene differential analysis revealed that the S100A8 and S100A9 genes were significantly downregulated after viaminate treatment. The results of qPCR and Western blotting confirmed that viaminate inhibited the expression of S100A8 and S100A9 genes and proteins in rat and HaCat cell acne models, while its downstream pathway MAPK (MAPK p38/JNK/ERK1/2) protein expression levels were suppressed. Additional administration of the S100A8 and S100A9 complex protein significantly reversed the inhibitory effect of viaminate on abnormal proliferation and keratinization levels in acne cell models. Conclusion: In summary, viaminate can improve acne by modulating S100A8 and S100A9 to inhibit MAPK pathway activation and inhibit keratinocyte proliferation and keratinization levels.

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Viaminate ameliorates Propionibacterium acnes-induced acne via inhibition of the TLR2/NF-κB and MAPK pathways in rats

Cited by 3 publications

References 36 publications

Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways

Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways

Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6- Mediated NF-κB and MAPKs Signaling Pathways

Viaminate Inhibits Propionibacterium Acnes-induced Abnormal Proliferation and Keratinization of HaCat Cells by Regulating the S100A8/S100A9- MAPK Cascade

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