Viable gynogenetic diploid Mytilus edulis (L.) larvae produced by ultraviolet light irradiation and cytochalasin B shock

Fairbrother, J. E.

doi:10.1016/0044-8486(94)90245-3

Cited by 16 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the pattern of decreasing fertilization rate with increasing irradiation time was shown as reported in H. discus hannai , 17,19 C. gigas 14 and M. edulis . 15 This result suggests that the ability of sperm to activate eggs was reduced with increasing irradiation dose. Because the change in fertilization rate is not observed in gynogenetic induction of fish species, 10–12 Kijima supposed that not only the nuclear genome but also the acrosome structure, which is absent in teleost fish, is damaged by UV irradiation in the Pacific abalone.…”

Section: Discussionmentioning

confidence: 93%

“…16 In M. edulis , 15 min irradiation provided the highest incidence of haploid embryos (UV intensity unshown). 15 Although the optimum dose of UV irradiation may vary among species and depends on the density and volume of sperm suspension and UV intensity, a reproducible protocol for genetical inactivation of C. gigas sperm by UV irradiation was obtained in this study.…”

Section: Discussionmentioning

confidence: 98%

“…Aneuploids shown here have also been reported in previous studies. 14,15,17,19 Inactivation of sperm chromosomes is suggested to be a continuous process. 14 As the mode at the haploid chromosome number appeared, cells showing low chromosome counts of 6–9 were seen in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, in molluscs, induction of viable diploid gynogenesis has been described only for a few species of Haliotis discus hannai , 13 Crassostrea gigas , 14 Mytilus edulis 15 and Mytilus galloprovincialis . 16 Although the gynogens survived to 10 and 6 months in C. gigas 14 and H. discus hannai , 13 respectively, there is no report on the production of adult gynogenetic individuals.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Osada

Kashihara³

et al. 2000

Fisheries Sci

View full text Add to dashboard Cite

SUMMARY: Effects of ultraviolet (UV) irradiation on genetical inactivation and morphological features of sperm were examined in the Pacific oyster. Ultraviolet light (254 nm) was effective for inactivating sperm chromosomes. Irradiation for 60 s at a UV intensity of 72 erg/mm 2 per s was the optimum dose to achieve haploid gynogenesis. The rates of the fertilization and the development of D‐shaped larvae decreased with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminated before reaching the D‐shaped stage. Electron microscopy showed clear destruction of the sperm acrosome and flagellum in the UV‐irradiated sperms. As the duration of irradiation increased, the acrosome of sperms tended to suffer greater damage until the sperms eventually lost their flagella. Abnormalities in these structures have appeared to account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV‐irradiated sperms.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Osada

Kashihara³

et al. 2000

Fisheries Sci

View full text Add to dashboard Cite

show abstract

“…Recently, the induction of gynogenetic diploids has been reported in the molluscs of Haliotis discus hannai , 18,19 Crassostrea gigas , 20 Mytilus edulis 21 and Mytilis galloprovincialis . 22 However, few cytological studies have described early fertilization events in eggs inseminated with ultraviolet (UV)‐irradiated sperm, even though they are the basis of the induction of gynogenetic mollusc individuals.…”

Section: Introductionmentioning

confidence: 99%

Cytological studies on artificially induced gynogenesis in the Pacific abalone

Osada

Kashihara³

et al. 2000

Fisheries Sci

View full text Add to dashboard Cite

SUMMARY: To obtain useful information about the mechanism of the induction of gynogenesis in the Pacific abalone, nuclear behavior in normal and gynogenetic eggs was examined during meiosis and first cleavage. After its incorporation into the egg cytoplasm, the UV‐irradiated sperm nucleus gradually dispersed and eventually developed into the male pronucleus, behaving like a normal sperm until contact with the female pronucleus. At mitotic prophase, the male pronucleus in the gynogenetic eggs did not form chromosomes like the female pronucleus, but became a dense chromatin body, which did not participate in karyokinesis. At completion of cytokinesis of the first cleavage, the UV‐irradiated sperm nucleus was seen in the cytoplasm of one of the two blastomeres near the cleavage furrow. As a clear haploid mode was observed in the frequency distribution of chromosome numbers in gynogenetic larval cells, it is suggested that the paternal genome in the gynogenetic eggs does not contribute to the next generation.

show abstract

Genetics in Aquaculture

2003

Bivalve Molluscs

View full text Add to dashboard Cite

Viable gynogenetic diploid Mytilus edulis (L.) larvae produced by ultraviolet light irradiation and cytochalasin B shock

Cited by 16 publications

References 18 publications

Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Cytological studies on artificially induced gynogenesis in the Pacific abalone

Genetics in Aquaculture

Contact Info

Product

Resources

About