Viable Bacteria Associated with Red Blood Cells and Plasma in Freshly Drawn Blood Donations

Damgaard, Christian; Magnussen, Karin; Enevold, Christian; Nilsson, Marie; Tolker‐Nielsen, Tim; Holmstrup, Palle; Nielsen, Claus Henrik

doi:10.1371/journal.pone.0120826

Cited by 104 publications

(122 citation statements)

References 46 publications

Supporting

Mentioning

109

Contrasting

Unclassified

Order By: Relevance

“…A recent study by Damgaard and colleagues (306) is of particular interest here. Recognizing the strong mismatch between the likelihood of an infection post-transfusion [very high (306)] and the likelihood of detecting culturable microbes in blood bank units (negligible, ca.…”

Section: Prevalence Of Dormant Persistent or Latent Bacteria In Infmentioning

confidence: 91%

“…Recognizing the strong mismatch between the likelihood of an infection post-transfusion [very high (306)] and the likelihood of detecting culturable microbes in blood bank units (negligible, ca. 0.1%) (306, 322), Damgaard et al reasoned that our methods of detecting and culturing these microbes might be the problem.…”

Section: Prevalence Of Dormant Persistent or Latent Bacteria In Infmentioning

confidence: 99%

See 1 more Smart Citation

A Dormant Microbial Component in the Development of Preeclampsia

Kell

Kenny

2016

Front. Med.

View full text Add to dashboard Cite

Preeclampsia (PE) is a complex, multisystem disorder that remains a leading cause of morbidity and mortality in pregnancy. Four main classes of dysregulation accompany PE and are widely considered to contribute to its severity. These are abnormal trophoblast invasion of the placenta, anti-angiogenic responses, oxidative stress, and inflammation. What is lacking, however, is an explanation of how these themselves are caused. We here develop the unifying idea, and the considerable evidence for it, that the originating cause of PE (and of the four classes of dysregulation) is, in fact, microbial infection, that most such microbes are dormant and hence resist detection by conventional (replication-dependent) microbiology, and that by occasional resuscitation and growth it is they that are responsible for all the observable sequelae, including the continuing, chronic inflammation. In particular, bacterial products such as lipopolysaccharide (LPS), also known as endotoxin, are well known as highly inflammagenic and stimulate an innate (and possibly trained) immune response that exacerbates the inflammation further. The known need of microbes for free iron can explain the iron dysregulation that accompanies PE. We describe the main routes of infection (gut, oral, and urinary tract infection) and the regularly observed presence of microbes in placental and other tissues in PE. Every known proteomic biomarker of “preeclampsia” that we assessed has, in fact, also been shown to be raised in response to infection. An infectious component to PE fulfills the Bradford Hill criteria for ascribing a disease to an environmental cause and suggests a number of treatments, some of which have, in fact, been shown to be successful. PE was classically referred to as endotoxemia or toxemia of pregnancy, and it is ironic that it seems that LPS and other microbial endotoxins really are involved. Overall, the recognition of an infectious component in the etiology of PE mirrors that for ulcers and other diseases that were previously considered to lack one.

show abstract

Section: Prevalence Of Dormant Persistent or Latent Bacteria In Infmentioning

confidence: 91%

Section: Prevalence Of Dormant Persistent or Latent Bacteria In Infmentioning

confidence: 99%

A Dormant Microbial Component in the Development of Preeclampsia

Kell

Kenny

2016

Front. Med.

View full text Add to dashboard Cite

show abstract

“…The majority of brain abscesses are caused by blood-borne dissemination of periodontal organisms, often following an initiating dental intervention 17 22 23 92. Judicious dental hygiene is recommended to reduce post-toothbrushing93 and post-procedural94 bacteraemias and potential later reactivation of dormant bacteria 95. The use of prophylactic antibiotics prior to dental examinations and procedures is supported by a clinical trial indicating efficacy of intravenous and oral antibiotic regimes in limiting transient dental bacteraemias after tooth extractions94 and other guidance 96.…”

Section: Section 2: Medical Managementmentioning

confidence: 99%

British Thoracic Society Clinical Statement on Pulmonary Arteriovenous Malformations

Shovlin

Condliffe

Donaldson³

et al. 2017

Thorax

114

165

View full text Add to dashboard Cite

Pulmonary arteriovenous malformations (PAVMs) are structurally abnormal vascular communications that provide a continuous right-to-left shunt between pulmonary arteries and veins. Their importance stems from the risks they pose (>1 in 4 patients will have a paradoxical embolic stroke, abscess or myocardial infarction while life-threatening haemorrhage affects 1 in 100 women in pregnancy), opportunities for risk prevention, surprisingly high prevalence and under-appreciation, thus representing a challenging condition for practising healthcare professionals. The driver for the current Clinical Statement was the plethora of new data since previous hereditary haemorrhagic telangiectasia (HHT) guidelines generated in 2006 and a systematic Cochrane Review for PAVM embolisation in 2011. The British Thoracic Society (BTS) identified key areas in which there is now evidence to drive a change in practice. Due to the paucity of data in children, this Statement focused on adults over 16 years. The Statement spans the management of PAVMs already known to be present (interventional and medical), screening and diagnosis (for PAVMs and HHT) and follow-up of patients following a first diagnosis, intervention or negative screen for PAVMs. The Good Practice Points (in bold) were generated for a target audience of general respiratory, medical and specialist clinicians and were approved by the BTS Standards of Care Committee, before formal peer review and public consultation. The Statement spans embolisation treatment, accessory medical management and issues related to the likelihood of underlying HHT.

show abstract

“…Kalfin reported 100% positivity of the blood cultures. Subsequently several other authors questioned the existence of the blood microbiome or DNAemia in healthy individuals, Domingue (1977) [5], (1997) [7], Nikkari et al (2001) [8], Mc Laughlin et al (2002) [9], Moriyama et al (2008) [10], Markova (2015) [11], Damgaard (2015) [12] Dimova et al (2017) [13], Gosiewski et al (2017) [2] and Kowarsky et al (2017) [3]. The authors reported supporting electron microscopy, cultural and molecular data in favour of the existence of the blood microbiota in healthy individuals.…”

Section: Tedeschi Et Al Reported On Incorporation Of Nucleosides Inmentioning

confidence: 99%

“…The authors reported supporting electron microscopy, cultural and molecular data in favour of the existence of the blood microbiota in healthy individuals. Hypothesis on the bacterial structure of the blood microbiota in healthy humans have been arisen [5] [6] [12], but cultures for exhaustive microbiota analysis remain a target. Rich bacterial diversity in the blood of healthy individuals was confirmed by 16S rRNA genes sequencing [14] and total RNA sequencing [15].…”

Section: Tedeschi Et Al Reported On Incorporation Of Nucleosides Inmentioning

confidence: 99%

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Panaiotov¹,

Filevski²,

Equestre³

et al. 2018

AiM

View full text Add to dashboard Cite

Background: On the analogy of the non-pathogenic microbiota found in oral cavity, skin and gastrointestinal tract, existence of blood microbiota was confirmed by DNA sequencing, but never deeply characterized. Hypothesis for the existence of dormant blood microbiota in healthy humans have been arisen and single species have been isolated. The aim of our study was to resuscitate and investigate the biodiversity of bacterial and fungal dormant blood microbiota in healthy individuals by blood culturing and NGS DNA sequencing. Results: Twenty eight blood samples of healthy individuals, seven for each blood type, were studied. Several culture media were tested. Blood microbiota resuscitation was performed in BHI broth supplemented with vitamin K 1 mg/ml, 2% sucrose, 0.25% sodium citrate and 0.2% yeastolate at 43˚C for 72 h. All tested blood samples were culture positive, as confirmed by Gram staining and TEM. TEM images demonstrated well defined cell structures. Analysis for bacterial and eukaryotic species was performed by 16S rRNA and ITS2 targeted sequencing. The obtained sequences were clustered (≥97% identity) in Operational Taxonomic Units (OTUs). Among cultured and uncultured samples we identified OTUs similarity with 47 bacterial orders belonging to 15 phyla and 39 fungi orders blonging to 2 phyla. For the first time we demonstrated isolation and sequencing identification of fungal blood microbiota in healthy individuals. Blood-group differences were identified among the bacterial microbiome compositions. Conclusion: The dormant blood microbiome is innate of the healthy individuals. Interventional strategies to bind the host blood microbiome with the states of health and disease remain an unmet research goal.

show abstract

Viable Bacteria Associated with Red Blood Cells and Plasma in Freshly Drawn Blood Donations

Cited by 104 publications

References 46 publications

A Dormant Microbial Component in the Development of Preeclampsia

A Dormant Microbial Component in the Development of Preeclampsia

British Thoracic Society Clinical Statement on Pulmonary Arteriovenous Malformations

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Contact Info

Product

Resources

About