Viability of vitrified mouse embryos using various cryoprotectant mixtures

Ishimori, H.; Takahashi, Yoshiyuki; Kanagawa, Hiroshi

doi:10.1016/0093-691x(92)90205-6

Cited by 36 publications

(11 citation statements)

References 14 publications

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“…The method is now widely utilized, but it requires a specially programmed freezer to control the cooling and warming rate accurately, and such an apparatus is quite expensive. On the other hand, vitrification (solidification of a solution without crystallization owing to elevated viscosity during the process of cooling) can be performed rapidly and simply without any such apparatus (Rail and Fahy, 1985;Scheffen et al, 1986;Kono and Tsunoda, 1988;Nakagata, 1989;Kasai et al, 1990;Nagashima et al, 1991;Schiewe et al, 1991;Ishimori et al, 1992). Recently, we developed a rapid and convenient mean of cryopreserving mouse preimplantation embryos (including pronucleate stage mouse eggs) by vitrification using sucrose or raffinose as a component of the vitrification solution (Tada et al, 1993a(Tada et al, ,b, 1994.…”

Section: Introductionmentioning

confidence: 98%

Production of transgenic mice by microinjection of DNA into vitrified pronucleate stage eggs

Tada¹,

Kasai²,

Ogawa

1995

Transgenic Research

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Vitrification is a technique for cryopreserving cells without crystallization due to elevation of the viscosity during the cooling process. We have developed a rapid and convenient mean of cryopreserving mouse preimplantation embryos by vitrification using a solution (hereafter named DPS) consisting of 2.75 M dimethylsulfoxide, 2.75 M propylene glycol and 1.0 M sucrose. In vitro fertilized pronucleate stage eggs were used because a large number of stage-matched eggs can be obtained at once. Only successfully fertilized eggs were collected and vitrified in DPS. After warming, two DNA constructs were injected into a total of 257 cryopreserved eggs, of which 175 (68%) survived the injection and were transferred into six recipients. All recipients became pregnant and gave birth to a total of 20 pups. When these DNA constructs were concomitantly injected into fresh eggs, 18% of eggs that were transferred developed into live pups, which was the same as the 18% figure for the cryopreserved eggs. With respect to transgenesis, 40% of the pups (8/20) developed from vitrified eggs were transgenic. In terms of the injected eggs that had been transferred, 4.5% of the 213 fresh eggs and 3.1% of the 112 vitrified eggs developed into transgenic mice. These results indicate that the efficiency of production of transgenic mice from vitrified eggs is comparable to that from fresh eggs.

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Section: Introductionmentioning

confidence: 98%

Production of transgenic mice by microinjection of DNA into vitrified pronucleate stage eggs

Tada¹,

Kasai²,

Ogawa

1995

Transgenic Research

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“…Data on permeation of sheep embryos (Szell el al, 1989) (Rail and Fahy, 1985;Rail, 1987;Ishimori et al, 1992;Isachenko et al, 1992;Cseh et al, 1992 …”

Section: Introductionmentioning

confidence: 99%

Design of vitrification solutions for the cryopreservation of embryos

Ali

Shelton²

1993

Reproduction

167

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“…The differences found in previous studies could be due to the use of only one CPA instead of two. Indeed, combinations of permeable CPAs have been successfully used in other studies to reduce the toxicity of individual agents during the vitrification of oocytes and embryos of several mammalian species [47][48][49][50].…”

Section: Discussionmentioning

confidence: 99%