Viability of plant cell suspensions exposed to homogeneous ultrasonic fields of different energy density and wave type

Böhm, Hannes R.; Anthony, Paul; Davey, M. R.; Briarty, L.G.; Power, J. B.; Lowe, K. C.; Benes, E.; Gröschl, Martin

doi:10.1016/s0041-624x(99)00166-3

Cited by 43 publications

(23 citation statements)

References 7 publications

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“…Studies by Doblhoffdier et al [66] suggested that there was no significant loss in the viability of mammalian cells suspended in a large ultrasonic resonator at operating power levels. This conclusion was also reached in a study by Bohm et al [67] using a similar chamber. These authors noted a significant difference between the response of cells to standing and travelling waves of similar frequency and pressure amplitude and a review of the behaviour of cells in such large resonators under different operating conditions was provided by Shirgaonkar et al [68].…”

Section: Cell Viabilitysupporting

confidence: 72%

Ultrasound assisted particle and cell manipulation on-chip

Mulvana

Cochran

Hill

2013

Advanced Drug Delivery Reviews

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Ultrasonic fields are able to exert forces on cells and other micron-scale particles, including microbubbles. The technology is compatible with existing lab-on-chip techniques and is complementary to many alternative manipulation approaches due to its ability to handle many cells simultaneously over extended length scales. This paper provides an overview of the physical principles underlying ultrasonic manipulation, discusses the biological effects relevant to its use with cells, and describes emerging applications that are of interest in the field of drug development and delivery on-chip.

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Section: Cell Viabilitysupporting

confidence: 72%

Ultrasound assisted particle and cell manipulation on-chip

Mulvana

Cochran

Hill

2013

Advanced Drug Delivery Reviews

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“…There is also evidence that the ultrasonic energy does not affect the viability of biological cells. 21,22 The technology is also promising for replacing magnetic beads in many bio-assays, providing finer control over beads, enhancing bead to surface interaction, and reducing cost by eliminating the specialised magnetic beads. The integrated optical wave guide permits fluorescence probing at the fluid/ reflector interface.…”

Section: Discussionmentioning

confidence: 99%

“…17,18 Although ultrasound can be used to destroy 19 or sonoporate cells, 20 there is growing evidence that at the intensity levels required for manipulation, cell viability 21,22 and their ability to proliferate 23 is maintained.…”

Section: Introductionmentioning

confidence: 99%

Flexible Acoustic Particle Manipulation Device with Integrated Optical Waveguide for Enhanced Microbead Assays

et al. 2009

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Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within ~100 nm of the waveguide, allowing the system to differentiate between targets bound to the beads and those unbound and still held in suspension. The radiation forces are generated in a standing-wave chamber that supports multiple acoustic modes, permitting particles to be both attracted to the waveguide surface and also repelled. To provide further control over particle position, a novel method of switching rapidly between different acoustic modes is demonstrated, through which particles are manipulated into an arbitrary position within the chamber. A novel type of assay is presented: a mixture of streptavidin coated and control beads are driven towards a biotin functionalised surface, then a repulsive force is applied, making it possible to determine which beads became bound to the surface. It is shown that the quarter-wave mode can enhance bead to surface interaction, overcoming potential barriers caused by surface charges. It is demonstrated that by measuring the time of flight of a microsphere across the device the bead size can be determined, providing a means of multiplexing the detection, potentially detecting a range of different target molecules, or varying bead mass.

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“…Soyabean, cowpea, maize, wheat, Ohio buckeye -various tissues SAAT* 55 kHz/ 0.2-100s/50W/10-20 × 2-4 mm clumps in 1 ml in 13×100 mm glass tube [15] Soyabean embryogenic callus SAAT* 55 kHz/ 0-300s/50W/10-20 × 2-4 mm clumps in 1 ml in 13×100 mm glass tube [16] Soyabean immature embryos SAAT* 55 kHz/ 0.1-10s/50W /10 cots in 0.5 ml in 1.5 ml microfuge tube [17] Soyabean cotyledonary nodes SAAT* 55 kHz/6-600s/50W/10 explants covered in Agrobacterium solution in 16×125 mm glass tube [18] Ohio buckeye SAAT* 55 kHz/ 0-60s/50W /10 embryogenic clumps in 1 ml in 13×100 mm glass tube [19] Robina pseudoacacia cotyledon SAAT* 30 kHz/ 60s/ 60 W /10 explants in 20 ml in 50 ml Falcon tube [20] Squash cotyledon explant multiple shoot regeneration 47 kHz/ 30-120s/ 35W/ 20 explants (420 mg) in 10 ml in 25×150 mm glass tube [21,22] Ultrasound can be supplied as a continuous waveform, or as pulses, propagating wave or standing wave, a fact little discussed in the literature of plant tissue culture. The waveform was of importance for survival of Petunia hybrida cell suspensions, as propagating waves reduce cell viability compared to standing wave fields of equal energy density [24]. The biophysics of ultrasound effects has been reviewed [25,26].…”

Section: The Generation Of Ultrasoundmentioning

confidence: 99%